[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations65513806
h-index2721
i10-index19586

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 20, Issue 5 (3-2013) ::
Journal of Ilam University of Medical Sciences 2013, 20(5): 112-120 Back to browse issues page
Phylogenetic identification of nitrogen-fixing bacteria isolated from the rhizosphere of asparagus plants using 16s rRNA and the effect of zinc on isolated strains
M Dadok , M Beglarian * 1, S Mehrabian , H Zali , M Zamanian azodi , M Salehi , Mh Maleki , Zargar
1- , biglaryan@yahoo.com
Abstract:   (17491 Views)
Background: According to the role of microorganisms in nitrogen stabilizer in the fertility of the soil and growth and development of plants, the use of chemical fertilizers zinc sulfate (ZnSo4) as micronutrient index in agricultur. Due to the toxic effects of zinc metal, the aim of this research, isolation and identification of nitrogen stabilizer microorganisms of asparagus rhizosphere and the effect of toxic zinc metal on the growth of bacteria. Material and Methods: In order to isolate microorganisms in nitrogen stabilizer, soil samples from the asparagus rhizosphere preparation in vitro lacks a nitrogen with a temperature of 30 ° c for 48 hours, was cultured, phylogenetic identify it using 16s rRNA do accepted and the MIC strain isolated in different concentrations of zinc metal was determined. Results: Rhizosphere soil samples from the plant among the asparagus a strain of bacteria was isolated, check the phylogenetic it using 16s rRNA species of Acinetobacter calcoaceticus was detected and isolated in disallowed MIC concentration 52.32 ppm zinc metal was evaluated. Conclusion: One of the rhizosphere nitrogen stabilizer strains in the plant asparagus, bacterium Acinetobacter calcoaceticus, increase the intake of zinc metal was microorganisms cause damage and the maximum allowable amount of zinc metal in this review, the concentration is less than the 52.32 ppm.
Keywords: Nitrogen fixation, Plant asparagus, 16s rRNA, Zinc sulfate (ZnSo4)
Full-Text [PDF 350 kb]   (6544 Downloads)    
Type of Study: Research | Subject: Medical microbiology
Received: 2013/03/17 | Accepted: 2013/05/13 | Published: 2013/05/13
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

dadok M, beglarian M, mehrabian S, zali H, zamanian azodi M, salehi M, et al . Phylogenetic identification of nitrogen-fixing bacteria isolated from the rhizosphere of asparagus plants using 16s rRNA and the effect of zinc on isolated strains. J. Ilam Uni. Med. Sci. 2013; 20 (5) :112-120
URL: http://sjimu.medilam.ac.ir/article-1-933-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 5 (3-2013) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.16 seconds with 41 queries by YEKTAWEB 4657