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:: Volume 20, Issue 4 (2-2013) ::
Journal of Ilam University of Medical Sciences 2013, 20(4): 192-199 Back to browse issues page
Analysis of Polymorphisms in Relation to Schizophrenia Susceptibility
S Haidari kashl, S Rahmanizade *1, F Ghasemvand, Mh Maleki
1- , saeed_r81@yahoo.com
Abstract:   (10405 Views)
Introduction: Schizophrenia is a compli-cated, debilitative mental disorder. Evide-nce is emerging for the association of pol-ymorphisms in PRODH gene and the increased risk of schizophrenia. In the pres-ent research we investigated the relation-nship between this gene and schizophrenia disease by means of a gene polymorphism using PCR-RFLP technique. Materials & Methods: Blood samples were prepared from 150 persons suffering from acute Schizophrenia and 160 healthy per-sons volunteering for this project. Based on intended SNP, a pair of primers was design-ned by Oligo7 program and the polymerase chain reaction (PCR) was performed on a thermocycler set. Then the PCR product was treated by appropriate restriction enzymes. Finally, the fragments of digested PCR product were electrophoresed on the gel agarose (4%) and migration pattern of resulted components were compared in he-althy and patient subjects, whereby obtain-ing genotypes of different persons in po-lymorphic position. We utilized SPSS 16.0 program for statistical investigation of the work and studied SNP 1945T>C and its relation with the disease in statistical population. Findings: The findings of the research sho-wed a meaningful relation between the occ-urrence of this nucleotide mutation and its frequency in patients.(P=0.001) Discussion & Conclusion: Final results of this work indicated that PRODH gene can be considered to be a significant candidate in the population under study as a factor influencing the occurrence of Schizoph-renia.
Keywords: schizophrenia, PRODH, proline
Full-Text [PDF 216 kb]   (4543 Downloads)    
Type of Study: Research | Subject: clinical biochemistry
Received: 2013/03/13 | Accepted: 2013/03/17 | Published: 2013/03/17
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haidari kashl S, rahmanizade S, ghasemvand F, maleki M. Analysis of Polymorphisms in Relation to Schizophrenia Susceptibility. Journal of Ilam University of Medical Sciences 2013; 20 (4) :192-199
URL: http://sjimu.medilam.ac.ir/article-1-909-en.html


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Volume 20, Issue 4 (2-2013) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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