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:: Volume 32, Issue 6 (2-2025) ::
Journal of Ilam University of Medical Sciences 2025, 32(6): 11-26 Back to browse issues page
Anticancer and Antiproliferative Effects of Combined Treatment with 5-Fluorouracil and Probiotic Lactobacillus Strains against Colorectal Cancer Cells
Sanaz Salek1 , Elham Moazamian * 2, Afshin Mohammadi Bardbori Mohammadi Bardbori3 , Seyedeh Azra Shamsdin4
1- Dept of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- Dept of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran , elhammoazamian@gmail.com
3- Dept of Toxicology and Pharmacology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
4- Gasteroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (283 Views)
Introduction:  The efficacy of 5-fluorouracil (5-FU) as a chemotherapy drug for colorectal cancer (CRC) is well-established. However, due to resistance to 5-FU and its associated complications, it is necessary to search for adjuvant therapies against CRC. Considering the anticancer potential of probiotic metabolites, this study assessed the anticancer effects of potential probiotic strains isolated from mule milk, camel milk, and the standard probiotic strain Lactobacillus rhamnosus GG (LGG), used alone and in combination with 5-FU, against the human colon cancer cell line (HT-29) and normal human embryonic kidney (HEK-293).
Materials & Methods: Biochemical and molecular techniques were implemented to identify the strains isolated from mule and camel milk. The MTT assay was also employed to assess the anticancer effects of the probiotic strains of Lactobacillus isolated from mule milk and camel milk and of LGG, used alone and in combination with 5-FU, on the two cell lines HT-29 and HEK-293. The data were statistically analyzed using analysis of variance, and significant differences between means were assessed using Tukey's post hoc test. Minitab 20 software performed all statistical calculations in the level of significance less than 0.05.
Results: The results demonstrated the potential probiotic properties of all the studied Lactobacillus strains. The combination of cell extracts from Lactobacillus strains with 5-FU effectively reduced the viability of HT-29 cells.  Nonetheless, this combination can reduce the cytotoxic effects of 5-FU on the viability of HEK-293 cells, thereby increasing their viability.
Conclusion: These results point to the possibility of using Lactobacillus strain cell extracts as an adjuvant therapy for cancer treatment
Keywords: Colorectal cancer, Lactobacillus, Probiotic, 5-Fluorouracil, Anticancer Activity
Full-Text [PDF 978 kb]   (138 Downloads)    
Type of Study: Research | Subject: biology
Received: 2024/06/13 | Accepted: 2024/10/27 | Published: 2025/02/3
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Salek S, Moazamian E, Mohammadi Bardbori A M B, Shamsdin S A. Anticancer and Antiproliferative Effects of Combined Treatment with 5-Fluorouracil and Probiotic Lactobacillus Strains against Colorectal Cancer Cells. J. Ilam Uni. Med. Sci. 2025; 32 (6) :11-26
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Volume 32, Issue 6 (2-2025) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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