Optimizing the hydroxyapatite nanocrystals derived from biological sources to increasing efficiency and quality

Abbasi, Mahsa; Derakhshankhah, Hossein; Kashanian, Soheila; Izadi, Zhila; Soleiman Beigi, Mohammad

[Home ] [Archive]

[ فارسی ]

Journal of Ilam University of Medical Sciences

Main Menu

Home

Journal Information

Articles archive

Publication Ethics

Peer Review Process

Indexing Databases

For Authors

For Reviewers

Subscription

Contact us

Site Facilities

Google Scholar Metrics

	All	Since 2019
Citations	6863	4096
h-index	28	22
i10-index	205	99

Search in website

Receive site information

Registered in

Volume 32, Issue 5 (12-2024)

Journal of Ilam University of Medical Sciences 2024, 32(5): 23-37

Back to browse issues page

Optimizing the hydroxyapatite nanocrystals derived from biological sources to increasing efficiency and quality

Mahsa Abbasi¹

, Hossein Derakhshankhah²

, Soheila Kashanian³

, Zhila Izadi ^*

⁴, Mohammad Soleiman Beigi¹

1- Dept of Chemistry, Faculty of Basic Sciences, Ilam University, Ilam, Iran
2- Pharmaceutical Sciences Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
3- Dept of Applied Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran
4- Pharmaceutical Sciences Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran , izadi.zhila@yahoo.com

Abstract: (137 Views)

Introduction: Hydroxyapatite is one of the most essential bioactive and biocompatible ceramics. Due to its unique properties and its structural similarity to bone, it has many applications in medicine, dentistry, and bone tissue engineering. Therefore, many methods and strategies have been studied to prepare hydroxyapatite. The purpose of this study was to optimize hydroxyapatite nanocrystals prepared from biological sources with higher efficiency and quality.
Materials & Methods: This study synthesized and analyzed hydroxyapatite from White Sea shells using simple and affordable methods like ultrasonic, hydrothermal, and thermal-ultrasonic decomposition. White seashells contain carbonate-calcium (C_aCO₃). For the synthesis of hydroxyapatite, environmentally friendly solvents such as water (green synthesis) and di ammonium hydrogen phosphate (ADP) were used as a source of phosphate. The data were analyzed using Graph Pad Prism V.10 statistical software, and the level of significance was considered less than 0.05.
Results: Hydroxyapatite was synthesized by the ultrasonic method and was chosen as the most appropriate. Because in the EDX results, the percentage of elements oxygen and phosphorus (O, P) in the hydroxyapatite synthesized by the ultrasonic method corresponds to the data of the standard card, HA~1.67, and for this reason, the ultrasonic method is better than the other two methods (hydrothermal and thermal-ultrasonic decomposition) and is more suitable. In FTIR, phosphate, hydroxide, and carbonate (PO₄^3-), OH, and CO₃^2- groups were observed, which indicate the synthesis of hydroxyapatite. With SEM and FE-SEM, the morphology and size of hydroxyapatite particles were observed to be between 16.5 and 70.80 nm. XRD analysis, crystallinity, and needle-like structure of hydroxyapatite were observed. The blood compatibility results showed that hydroxyapatite had no significant hemolysis. Examining the cell compatibility of hydroxyapatite on bone-like cells (MG63) showed a survival rate of over 90%, and cell proliferation was statistically (Graph Pad Prism 10) significant (P < 0.05).
Conclusion: One of the most effective methods for preparing hydroxyapatite involves conducting the reactions at the bottom; the ultrasonic method's affordability and cost-effectiveness are among its positive features, as it can generate multiple processes throughout the production process. The results demonstrated that this method produced a material with a hexagonal structure, nanometer size, and suitable biocompatibility.

Keywords: Hydroxyapatite, Ultrasonic method, Biocompatibility

Full-Text [PDF 1110 kb] (67 Downloads)

Type of Study: Research | Subject: research
Received: 2024/04/29 | Accepted: 2024/06/16 | Published: 2024/12/5

References

1. Chitra S, Rajeshkumar S, Ramya R, Bargavi P, Balakumar S. Probing the biocompatibility and antioxidant properties of Cissus quadrangularis conjugated bioactive glass and hydroxyapatite towards regeneration application. Inorg Chem Commun 2023;157:111398. doi: 10.1016/j.inoche.2023.111398.

2. Abdian N, Etminanfar M, Sheykholeslami SOR, Hamishehkar H, Khalil-Allafi J. Preparation and characterization of chitosan/hydroxyapatite scaffolds containing mesoporous SiO2-HA for drug delivery applications. Mater Chem Phys 2023;301:127672. doi: 10.1016/j.matchemphys.2023.127672.

3. Arokiasamy P, Abdullah MMAB, Abd Rahim SZ, Luhar S, Sandu AV, Jamil NH, et al. Synthesis methods of hydroxyapatite from natural sources: A review. Ceram Int 2022;48:14959-79. doi: 10.1016/j.ceramint.2022.03.064.

4. Pavlychev A, Brykalova X, Korneev A, Cherny A, Kornilov N. Specific Features of the Crystal Structure of Calcium Hydroxyapatite in Native Bone Tissue. Crystallogr Rep 2024;69:38-44. doi:10.1134/S1063774523601326.

5. Xiao D, Zhang J, Zhang C, Barbieri D, Yuan H, Moroni L, et al. The role of calcium phosphate surface structure in osteogenesis and the mechanisms involved. Acta Biomater 2020;106:22-33. doi: 10.1016/j.actbio.2019.12.034.

6. Yang-Zhou CH, Cao JX, Dong SS, Chen SH, Michael RN. Phosphorus co-existing in water: a new mechanism to boost boron removal by calcined oyster shell powder. Molecules 2021;27:54. doi: 10.3390/molecules27010054.

7. Sricharoen P, Limchoowong N, Nuengmatcha P, Chanthai S. Ultrasonic-assisted recycling of Nile tilapia fish scale biowaste into low-cost nano-hydroxyapatite: Ultrasonic-assisted adsorption for Hg2+ removal from aqueous solution followed by “turn-off” fluorescent sensor based on Hg2+-graphene quantum dots. Ultrason Sonochem 2020;63:104966. doi: 10.1016/j.ultsonch.2020.104966.

8. Anandan D, Jaiswal AK. Synthesis methods of hydroxyapatite and biomedical applications: an updated review. ACSJ 2024;60:663-79. doi: 10.1007/s41779-023-00943-2.

9. Indira J, Malathi K. Comparison of template mediated ultrasonic and microwave irradiation method on the synthesis of hydroxyapatite nanoparticles for biomedical applications. Mater Today 2022;51:1765-9. doi: 10.1016/j.matpr.2021.03.028.

10. Osuchukwu OA, Salihi A, Abdullahi I, Etinosa PO, Obada DO. A comparative study of the mechanical properties of sol-gel derived hydroxyapatite produced from a novel mixture of two natural biowastes for biomedical applications. Sci Rep 2022;12:17968. doi: 10.1038/s41598-022-22888-5.

11. Hajibeygi M, Mousavi M, Shabanian M, Habibnejad N, Vahabi H. Design and preparation of new polypropylene/magnesium oxide micro particles composites reinforced with hydroxyapatite nanoparticles: A study of thermal stability, flame retardancy and mechanical properties. Mater Chem Phys 2021;258:123917. doi: 10.1016/j.matchemphys.2020.123917.

12. Yinka KM, Olayiwola AJ, Sulaiman A, Ali A, Iqbal F. Preparation and characterization of hydroxyapatite powder for biomedical applications from giant African land snail shell using a hydrothermal technique. EASR 2020;47. doi: 10356/66023.

13. Lin DJ, Lin HL, Haung SM, Liu SM, Chen WC. Effect of ph on the in vitro biocompatibility of surfactant-assisted synthesis and hydrothermal precipitation of rod-shaped nano-hydroxyapatite. Polymers 2021;13:2994. doi: 10.3390/polym13172994.

14. Molino G, Palmieri MC, Montalbano G, Fiorilli S, Vitale-Brovarone C. Biomimetic and mesoporous nano-hydroxyapatite for bone tissue application: A short review. Biomed Mater 2020;15:022001. doi: 10.1088/1748-605X/ab5f1a.

15. Kadu K, Kowshik M, Ramanan SR. Does the nanoparticle morphology influence interaction with protein: A case study with hydroxyapatite nanoparticles. Mater Today Commun 2021;26:102172. doi: 10.1016/j.mtcomm.2021.102172.

16. Upadhyay P, Ullah A. Facile synthesis of hydroxyapatite nanoparticles from eggshell biowaste using Azadirachta indica extract as a green template. New J Chem 2024;48:1424-35. doi: 10.1039/D3NJ01715J.

17. Prakash VCA, Venda I, Thamizharasi V, Sathya E. A new attempt on synthesis of spherical nano hydroxyapatite powders prepared by dimethyl sulfoxide-poly vinyl alcohol assisted microemulsion method. Mater Chem Phys 2021;259:124097. doi: 10.1016/j.matchemphys.2020.124097.

18. Orasugh JT, Ghosh SK, Chattopadhyay D. Nanofiber-reinforced biocomposites. Fiber-reinforced nanocomposites: fundamentals and applications: Elsevier; 2020. p. 199-233. doi: 10.1016/B978-0-12-819904-6.00010-4.

19. Raja PB, Munusamy KR, Perumal V, Ibrahim MNM. Characterization of nanomaterial used in nanobioremediation. Nano-bioremediation: fundamentals and applications: Elsevier; 2022. p. 57-83. doi: 10.1016/B978-0-12-823962-9.00037-4.

20. Jia B, Yang H, Han Y, Zhang Z, Qu X, Zhuang Y, et al. In vitro and in vivo studies of Zn-Mn biodegradable metals designed for orthopedic applications. Acta Biomater 2020;108:358-72. doi: 10.1016/j.actbio.2020.03.009.

21. Kumar K, Das A, Prasad SB. Biodegradable metal matrix composites for orthopedic implant applications: A review. Advances in Engineering Materials: Select Proceedings of FLAME 2020. 2021:557-65. doi: 10.1007/978-981-33-6029-7_52.

22. Díaz-Cuenca A, Rabadjieva D, Sezanova K, Gergulova R, Ilieva R, Tepavitcharova S. Biocompatible calcium phosphate-based ceramics and composites. Mater Today 2022;61:1217-25. doi: 10.1016/j.matpr.2022.01.329.

23. Josephin A, Intan N, Basari B, Rahman SF, editors. Extraction of collagen and hydroxyapatite from fish for bone scaffold: A review. AIP Conference Proceedings; 2024: AIP Publishing. doi: 10.1063/5.0198630.

24. Sans J, Sanz V, Puiggalí J, Turon P, Alemán C. Controlled anisotropic growth of hydroxyapatite by additive-free hydrothermal synthesis. Crystal Growth Design 2020;21:748-56. doi: 10.1021/acs.cgd.0c00850.

25. Verma R, Mishra SR, Gadore V, Ahmaruzzaman M. Hydroxyapatite-based composites: Excellent materials for environmental remediation and biomedical applications. Adv Colloid Interface Sci 2023:102890. doi: 10.1016/j.cis.2023.102890.

26. Zastulka A, Clichici S, Tomoaia-Cotisel M, Mocanu A, Roman C, Olteanu CD, et al. Recent trends in hydroxyapatite supplementation for osteoregenerative purposes. Materials 2023;16:1303. doi: 10.3390/ma16031303.

27. Rajkumar P, Sarma BK. Role of Zn and Mg substitutions on the mechanical behaviour of biomimetic hydroxyapatite and insight of the emergence of hydroxyapatite-ZnO nanocomposite. Mater Charact 2021;176:111107. doi: 10.1016/j.matchar.2021.111107.

28. Filip DG, Surdu VA, Paduraru AV, Andronescu E. Current development in biomaterials—hydroxyapatite and bioglass for applications in biomedical field: a review. J Funct Biomater 2022;13:248. doi: 10.3390/jfb13040248.

29. Pu'ad NM, Haq RA, Noh HM, Abdullah H, Idris M, Lee T. Synthesis method of hydroxyapatite: A review. Mater Today 2020;29:233-9. doi: 10.1016/j.matpr.2020.05.536.

30. Gani MA, Budiatin AS, Shinta DW, Ardianto C, Khotib J. Bovine hydroxyapatite-based scaffold accelerated the inflammatory phase and bone growth in rats with bone defect. J Appl Biomater Funct Mater 2023;21:22808000221149193. doi: 10.1177/22808000221149193.

31. Surya P, Nithin A, Sundaramanickam A, Sathish M. Synthesis and characterization of nano-hydroxyapatite from Sardinella longiceps fish bone and its effects on human osteoblast bone cells. J Mech Behav Biomed Mater 2021;119:104501. doi: 10.1016/j.jmbbm.2021.104501.

32. Khosalim IP, Zhang YY, Yiu CKY, Wong HM. Synthesis of a graphene oxide/agarose/hydroxyapatite biomaterial with the evaluation of antibacterial activity and initial cell attachment. Sci Rep 2022;12:1971. doi: 10.1038/s41598-022-06020-1.

33. Ijaz I, Gilani E, Nazir A, Bukhari A. Detail review on chemical, physical and green synthesis, classification, characterizations and applications of nanoparticles. GCLR 2020;13:223-45. doi: 10.1080/17518253.2020.1802517.

34. Elbasuney S. Green synthesis of hydroxyapatite nanoparticles with controlled morphologies and surface properties toward biomedical applications. J Inorg Organomet Polym Mater 2020;30:899-906. doi: 10.1007/s10904-019-01247-4.

35. Vinayagam R, Pai S, Murugesan G, Varadavenkatesan T, Kaviyarasu K, Selvaraj R. Green synthesized hydroxyapatite nanoadsorbent for the adsorptive removal of AB113 dye for environmental applications. Environ Res 2022;212:113274. doi: 10.1016/j.envres.2022.113274.

Send email to the article author

Add your comments about this article

Ethics code: IR.KUMS.REC.1403.024

Mendeley

Zotero

RefWorks

Abbasi M, Derakhshankhah H, Kashanian S, Izadi Z, Soleiman Beigi M. Optimizing the hydroxyapatite nanocrystals derived from biological sources to increasing efficiency and quality. J. Ilam Uni. Med. Sci. 2024; 32 (5) :23-37
URL: http://sjimu.medilam.ac.ir/article-1-8278-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 32, Issue 5 (12-2024)

Back to browse issues page

Persian site map - English site map - Created in 0.15 seconds with 41 queries by YEKTAWEB 4671