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:: Volume 32, Issue 3 (8-2024) ::
Journal of Ilam University of Medical Sciences 2024, 32(3): 12-20 Back to browse issues page
Assessment of malondialdehyde levels and antioxidant enzyme activity in an astaxanthin-treated human neuroblastoma cell line
Nasim Beigi Boroujeni * 1, Maryam Hormozi2
1- Razi Herbal Medicines Research Center, Dept of Anatomy, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran , nasim_boroujeni@yahoo.com
2- Razi Herbal Medicines Research Center, Dept of Biochemistry, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract:   (407 Views)
Introduction:  Oxidative stress is involved in the pathophysiology of neurodegenerative diseases, such as Alzheimer's, Parkinson's, Huntington's, amyotrophic lateral sclerosis, and multiple sclerosis. It seems that the intake of exogenous antioxidants may be effective in preventing, treating, and reducing the complications of these diseases. Astaxanthin is a carotenoid pigment with antioxidant and anti-inflammatory properties, protecting the nervous system. The present study aimed to assess the effect of astaxanthin on the amount of malondialdehyde and the activity of antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase) after oxidative stress with hydrogen peroxide in the human neuroblastoma cell line BE (2)-C.
Material & Methods: Human neuroblastoma cells were treated in this study with different concentrations of astaxanthin (25, 50, and 100 μM) or 50 μM ascorbic acid (positive control) for 24 h. To induce oxidative stress, they were exposed to hydrogen peroxide at a concentration of 400 μM for 2 h. There was also a control group without treatment and without inducing oxidative stress. The amount of malondialdehyde as an index of oxidative stress and the activity of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase were measured using calorimetric methods.
Results: The obtained results demonstrated that the malondialdehyde concentration was significantly reduced in the groups treated with different concentrations of astaxanthin and ascorbic acid compared to the hydrogen peroxide group (P<0.05). The activities of superoxide dismutase, catalase, and glutathione peroxidase also increased significantly in these groups compared to the group with oxygenated water (P<0.05).
Discussion & Conclusion: Astaxanthin appears to counteract the oxidative stress caused by hydrogen peroxide by lowering malondialdehyde levels and increasing the activity of antioxidant enzymes in BE (2) C cells, thereby protecting the cells from the impact of oxidative stress
Keywords: Antioxidant enzymes, Astaxanthin, Neuroblastoma cell line, Oxidative stress
Full-Text [PDF 1400 kb]   (227 Downloads)    
Type of Study: Research | Subject: clinical biochemistry
Received: 2024/01/3 | Accepted: 2024/03/10 | Published: 2024/08/5
References
1. Olufunmilayo EO, Gerke-Duncan MB, Holsinger RD. Oxidative stress and antioxidants in neurodegenerative disorders. Antioxidants 2023; 18;12:517. doi:10.3390/antiox12020517.
2. Bonet-Costa V, Pomatto LC, Davies KJ. The proteasome and oxidative stress in Alzheimer's disease. Antioxid Redox Signal 2016 ; 25:886-901. doi:10.1089/ars.2016.6802.
3. Carocci A, Catalano A, Sinicropi MS, Genchi G. Oxidative stress and neurodegeneration: the involvement of iron. Biometals 2018; 31:715-35. doi:10.1007/s10534-018-0126-2.
4. Haider L. Inflammation, iron, energy failure, and oxidative stress in the pathogenesis of multiple sclerosis. Oxid Med Cell Longev 2015; 2015: 725370. doi:10.1155/2015/725370.
5. Picón-Pagès P, Garcia-Buendia J, Munoz FJ. Functions and dysfunctions of nitric oxide in brain. Biochim Biophys Acta (BBA)Mol Basis Dis 2019; ;1865:1949-67. doi: 10.1016/j.bbadis.2018.11.007.
6. Shichiri M. The role of lipid peroxidation in neurological disorders. J Clin Biochem Nutr 2014;54:151-60. doi:10.3164/jcbn.14-10.
7. Floyd RA, Carney JM. Free radical damage to protein and DNA: mechanisms involved and relevant observations on brain undergoing oxidative stress. Ann Neuro 1992;32:S22-7. doi: 10.1002/ana.410320706.
8. Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. Eur J Med Chem 2015;97:55-74. doi: 10.1016/j.ejmech.2015.04.040.
9. Higuera-Ciapara I, Felix-Valenzuela L, Goycoolea FM. Astaxanthin: a review of its chemistry and applications. Crit Rev Food Sci Nutr 2006; 46:185-96. doi: 10.1080/10408690590957188.
10. Pashkow FJ, Watumull DG, Campbell CL. Astaxanthin: a novel potential treatment for oxidative stress and inflammation in cardiovascular disease. Am J Cardiol 2008;22;101:S58-68. doi: 10.1016/j.amjcard.2008.02.010.
11. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54. doi: 10.1006/abio.1976.9999.
12. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8. doi: 10.1016/0003-2697(79)90738-3.
13. Boussiba S, Bing W, Yuan JP, Zarka A, Chen F. Changes in pigments profile in the green alga Haeamtococcus pluvialis exposed to environmental stresses. Biotechnol Lett 1999; 21:601-4. doi:10.1023/A:1005507514694.
14. Stewart JS, Lignell Å, Pettersson A, Elfving E, Soni MG. Safety assessment of astaxanthin-rich microalgae biomass: Acute and subchronic toxicity studies in rats. Food Chem Toxico 2008; 46:3030-6. doi:10.1016/j.fct.2008.05.038.
15. Ranga Rao A. Production of astaxanthin from cultured green alga Haematococcus pluvialis and its biological activities (Doctoral dissertation, University of Mysore).2011
16. Ambati RR, Phang SM, Ravi S, Aswathanarayana RG. Astaxanthin: Sources, extraction, stability, biological activities and its commercial applications—A review. Mar Drugs 2014;12:128-52. doi:10.3390/md12010128.
17. Balietti M, Giannubilo SR, Giorgetti B, Solazzi M, Turi A, Casoli T, et al. The effect of astaxanthin on the aging rat brain: gender‐related differences in modulating inflammation. J Sci Food Agric 2016;96:615-8. doi:10.1002/jsfa.7131.
18. Franceschelli S, Pesce M, Ferrone A, De Lutiis MA, Patruno A, Grilli A, et al. Astaxanthin treatment confers protection against oxidative stress in U937 cells stimulated with lipopolysaccharide reducing O2− production. PLoS One 2014;9:e88359. doi:10.1371/journal.pone.0088359
19. Freeman BA, Crapo JD. Biology of disease: free radicals and tissue injury. Lab Invest 1982;47:412-26.
20. Li X, Qi Z, Zhao L, Yu Z. Astaxanthin reduces type 2 diabetic associated cognitive decline in rats via activation of PI3K/Akt and attenuation of oxidative stress. Mol Med Rep 2016;13:973-9. doi: 10.3892/mmr.2015.4615.
21. Ravi Kumar S, Narayan B, Sawada Y, Hosokawa M, Miyashita K. Combined effect of astaxanthin and squalene on oxidative stress in vivo. Mol Cell Biochem 2016 ;417:57-65. doi: 10.1007/s11010-016-2713-2.
22. Tripathi DN, Jena GB. Intervention of astaxanthin against cyclophosphamide-induced oxidative stress and DNA damage: a study in mice. Chem Biol Interact 2009 ;180:398-406. doi: 10.1016/j.cbi.2009.03.017.
23. Wu W, Wang X, Xiang Q, Meng X, Peng Y, Du N, et al. Astaxanthin alleviates brain aging in rats by attenuating oxidative stress and increasing BDNF levels. Food Funct 2014;5:158-66. doi: 10.1039/c3fo60400d.
24. Hashimoto H, Arai K, Hayashi S, Okamoto H, Takahashi J, Chikuda M, et al. Effects of astaxanthin on antioxidation in human aqueous humor. J Clin Biochem Nutr 2013;53:1-7. doi: 10.3164/jcbn.13-6.
25. Chang CS, Chang CL, Lai GH. Reactive oxygen species scavenging activities in a chemiluminescence model and neuroprotection in rat pheochromocytoma cells by astaxanthin, beta-carotene, and canthaxanthin. Kaohsiung J Med Sci 2013 ;29: 412-21. doi: 10.1016/j.kjms.2012.12.002.
26. Wang HQ, Sun XB, Xu YX, Zhao H, Zhu QY, Zhu CQ. Astaxanthin upregulates heme oxygenase-1 expression through ERK1/2 pathway and its protective effect against beta-amyloid-induced cytotoxicity in SH-SY5Y cells. Brain Res 2010 ;1360:159-67. doi: 10.1016/j.brainres.2010.08.100.
27. Lee DH, Kim CS, Lee YJ. Astaxanthin protects against MPTP/MPP+-induced mitochondrial dysfunction and ROS production in vivo and in vitro. Food Chem Toxicol 2011;49:271-80. doi:10.1016/j.fct.2010.10.029.
28. Al-Amin MM, Reza HM, Saadi HM, Mahmud W, Ibrahim AA, Alam MM, et al. Astaxanthin ameliorates aluminum chloride-induced spatial memory impairment and neuronal oxidative stress in mice. Eur JPharmacol 2016 ;777:60-9. doi: 10.1016/j.ejphar.2016.02.062.
29. Lu Y, Xie T, He XX, Mao ZF, Jia LJ, Wang WP, et al. Astaxanthin rescues neuron loss and attenuates oxidative stress induced by amygdala kindling in adult rat hippocampus. Neurosci Lett 2015; 597:49-53. doi:10.1016/j.neulet.2015.04.018.
30. Zhang XS, Zhang X, Zhang QR, Wu Q, Li W, Jiang TW, et al. Astaxanthin reduces matrix metalloproteinase-9 expression and activity in the brain after experimental subarachnoid hemorrhage in rats. Brain Res 2015 ;1624:113-24. doi:10.1016/j.brainres.2015.07.020.
31. Masoudi A, Dargahi L, Abbaszadeh F, Pourgholami MH, Asgari A, Manoochehri M, et al. Neuroprotective effects of astaxanthin in a rat model of spinal cord injury. Behav Brain Res 2017 ;329:104-10. doi:10.1016/j.bbr.2017.04.026.
32. Lobos P, Bruna B, Cordova A, Barattini P, Galáz JL, Adasme T, et al. Astaxanthin Protects Primary Hippocampal Neurons against Noxious Effects of Aβ‐Oligomers. Neural Plast 2016;3456783. doi: 10.1155/2016/3456783.
33. Zhang XS, Zhang X, Wu Q, Li W, Wang CX, Xie GB, et al. Astaxanthin offers neuroprotection and reduces neuroinflammation in experimental subarachnoid hemorrhage. J Surg Res 2014; 192: 206-13. doi: 10.1016/j.jss.2014.05.029.
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Beigi Boroujeni N, Hormozi M. Assessment of malondialdehyde levels and antioxidant enzyme activity in an astaxanthin-treated human neuroblastoma cell line. J. Ilam Uni. Med. Sci. 2024; 32 (3) :12-20
URL: http://sjimu.medilam.ac.ir/article-1-8198-en.html
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Volume 32, Issue 3 (8-2024) Back to browse issues page
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