Investigating the effects of buforin II coding gene on the expression of lncRNAs PVT1, EGOT and LINC00312 in kidney cancer cell line

Amiri-Farsani, Maryam; Doosti, Abbas

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Volume 32, Issue 2 (6-2024)

Journal of Ilam University of Medical Sciences 2024, 32(2): 32-43

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Investigating the effects of buforin II coding gene on the expression of lncRNAs PVT1, EGOT and LINC00312 in kidney cancer cell line

Maryam Amiri-Farsani¹

, Abbas Doosti ^*

1- Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran & Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran & Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , msafarpour84@yahoo.com

Abstract: (217 Views)

Introduction: Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults. The aim of this study is to investigate the effects of the buforin II gene on the expression of lncRNAs PVT1, EGOT and LINC00312 in ACHN kidney cancer cells.
Material & Methods: Recombinant plasmid pcDNA3.1(+) containing buforin II gene and empty plasmid pcDNA3.1(+) were introduced into E. coli strain TOP10 by heat shock method and then purified. Then both mentioned plasmids were introduced into ACHN cells by lipofection method and screening was done by neomycin antibiotic. Finally, real time RT-PCR reaction was performed in order to investigate the expression change of EGOT, PVT1 and LINC00312 lncRNAs.
Results: After lipofection, the transfected cells grew in the culture medium containing neomycin antibiotic. The real time RT-PCR reaction showed that the expression of buforin II gene in ACHN kidney cancer cells caused a significant increase in the expression of lncRNAs EGOT (P=0.0033) and LINC00312 (P=0.0272) and a significant decrease in the expression of PVT1 (P=0.0278).
Discussion & Conclusion: Considering that the presence of buforin II gene causes a significant change in the expression of lncRNAs EGOT, PVT1 and LINC00312, it is possible that it can activate cell pathways including apoptosis.

Keywords: Renal cell carcinoma, buforin II, ACHN cell line

Full-Text [PDF 1427 kb] (129 Downloads)

Type of Study: Research | Subject: Molecular Genetics
Received: 2023/09/30 | Accepted: 2024/01/28 | Published: 2024/06/4

References

1. Chen Z, Han F, Du Y, Shi H, Zhou W. Hypoxic microenvironment in cancer: molecular mechanisms and therapeutic interventions. Signal Transduct Target Ther 2023; 17;8:70. doi: 10.1038/s41392-023-01332-8.

2. Colomer‐Lahiguera S, Steimer M, Ellis U, Eicher M, Tompson M, Corbière T, et al. Patient and public involvement in cancer research: A scoping review. Cancer Med 2023; 16. doi: 10.1002/cam4.6200.

3. Nabi S, Kessler ER, Bernard B, Flaig TW, Lam ET. Renal cell carcinoma: a review of biology and pathophysiology. F1000Res 2018;7: 1-10. doi: 10.12688/f1000research.13179.1.

4. Safarpour-Dehkordi M, Doosti A, Jami MS. Integrative analysis of lncRNAs in kidney cancer to discover a new lncRNA (LINC00847) as a therapeutic target for staphylococcal enterotoxin tst gene. Cell J 2020;22:101. doi: 10.22074/cellj.2020.6996.

5. Jin L, Quan J, Pan X, He T, Hu J, Li Y, et al. Identification of lncRNA EGOT as a tumor suppressor in renal cell carcinoma. Mol Med Rep 2017;16:7072-9. doi: 10.3892/mmr.2017.7470.

6. Guo Z, Wang YH, Xu H, Yuan CS, Zhou HH, Huang WH, et al. LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma. Cell Death Dis 2021;12:69. doi: 10.1038/s41419-020-03302-2.

7. Li M, Wang Y, Cheng L, Niu W, Zhao G, Raju JK, et al. Long non-coding RNAs in renal cell carcinoma: a systematic review and clinical implications. Oncotarget 2017;8:48424-35.doi: 10.18632/oncotarget.17053.

8. Schweizer F. Cationic amphiphilic peptides with cancer-selective toxicity. Eur J Pharmacol 2009; 625:190-4. doi:10.1016/j.ejphar.2009.08.043.

9. Park CB, Yi KS, Matsuzaki K, Kim MS, Kim SC. Structure–activity analysis of buforin II, a histone H2A-derived antimicrobial peptide: the proline hinge is responsible for the cell-penetrating ability of buforin II. Proc Natl Acad Sci U S A 2000; 97:8245-50. doi: 10.1073/pnas.150518097.

10. Lee HS, Park CB, Kim JM, Jang SA, Park IY, Kim MS. Mechanism of anticancer activity of buforin IIb, a histone H2A-derived peptide. Cancer Lett 2008; 271:47-55. doi: 10.1016/j.canlet.2008.05.041.

11. Safarpour M, Kazemi Z, Doosti E, Doosti A. Cloning tagD gene from helicobacter pylori in PFLAG-CMV-3 eukaryotic vector to generate a DNA vaccine. JIUMS 2016; 14:43-50. 10.29252/JMJ.14.4.43.

12. Seim I, Jeffery PL, Thomas PB, Walpole CM, Maugham M, Fung JN, et al. Multi-species sequence comparison reveals conservation of ghrelin gene-derived splice variants encoding a truncated ghrelin peptide. Endocrine 2016; 52:609-17. doi: 10.1007/s12020-015-0848-7.

13. Safarpour-Dehkordi M, Samimi-Dehkordi N, Asgari M, Khademi R, Kabirian-Dehkordi M, Amiri M, et al. Co-expression network analysis for the identification of potential prostate cancer genes and in vitro confirmation of their expression in cell model in the presence of Staphylococcal tsst-1 gene. Nucleosides Nucleotides Nucleic Acids 2023; 17:1-6. doi:10.1080/15257770.2023.2249544.

14. Safarpour-Dehkordi M, Doosti A, Jami MS. Impacts of the Staphylococcal Enterotoxin H on the Apoptosis and lncRNAs in PC3 and ACHN. Mol Gen Microbiol Virol 2020; 35:180-8. doi: 10.3103/S0891416820030076.

15. Li H, Li X, Bai M, Suo Y, Zhang G, Cao X. Matrine inhibited proliferation and increased apoptosis in human breast cancer MCF-7 cells via upregulation of Bax and downregulation of Bcl-2. Int J Clin Exp Patho 2015; 8:14793.‌

16. Zhou M, Zou X, Cheng K, Zhong S, Su Y, Wu T, et al. The role of cell‐penetrating peptides in potential anti‐cancer therapy. Clin Transl Med 2022;12: e822. doi: 10.1002/ctm2.822.

17. Vakili B, Jahanian-Najafabadi A. Application of Antimicrobial Peptides in the Design and Production of Anticancer Agents. Int J Pept Res Ther 2023; 20; 29:28.

18. Parker JP, Devocelle M, Morgan MP, Marmion CJ. Derivatisation of buforin IIb, a cationic henicosapeptide, to afford its complexation to platinum (ii) resulting in a novel platinum (ii)–buforin IIb conjugate with anti-cancer activity. Dalton Trans 2016;45:13038-41. doi: 10.1039/c6dt01510g.

19. Li M, Wang Y, Cheng L, Niu W, Zhao G, Raju JK, et al. Long non-coding RNAs in renal cell carcinoma: a systematic review and clinical implications. Oncotarget 2017; 7; 8:48424. doi: 10.18632/oncotarget.17053.

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Amiri-Farsani M, Doosti A. Investigating the effects of buforin II coding gene on the expression of lncRNAs PVT1, EGOT and LINC00312 in kidney cancer cell line. J. Ilam Uni. Med. Sci. 2024; 32 (2) :32-43
URL: http://sjimu.medilam.ac.ir/article-1-8108-en.html

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