Effect of maceration time and type of solvent on the content of phenolic compounds and antioxidant activity of Digitalis purpurea L

Rezaie allolo, Afsoon; SaniKhani, Mohsen; Khairy, Aziz alah; Yaghoobi, Maliheh

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Volume 31, Issue 6 (2-2024)

Journal of Ilam University of Medical Sciences 2024, 31(6): 11-22

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Effect of maceration time and type of solvent on the content of phenolic compounds and antioxidant activity of Digitalis purpurea L

Afsoon Rezaie allolo¹

, Mohsen SaniKhani ^*

², Aziz alah Khairy¹

, Maliheh Yaghoobi³

1- Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2- Faculty of Agriculture, University of Zanjan, Zanjan, Iran , sani@znu.ac.ir
3- Dept of Engineering, Faculty of Chemical Engineering, University of Zanjan, Zanjan, Iran

Abstract: (1110 Views)

Introduction: The extraction of chemical compounds from plants is influenced by various factors, such as methodology and duration of extraction, sample preparation, and the type and concentration of solvent used. This study was performed to investigate the effect of extraction time and type of solvent on the contents of phenolic compounds, flavonoids, and antioxidant activity of Digitalis purpurea L. plant.
Material & Methods: An experiment based on a completely randomized design in a factorial arrangement with three replications was conducted at the Horticulture Laboratory at the University of Zanjan, Zanjan, Iran, in 2021. The experimental treatments included two levels of extraction duration (24 and 48 h) as the main factor, and two levels of methanol, ethanol, and acetone solvents (100% and 80%), compared with the control (distilled water), as sub-factors. Extraction of purple foxglove leaf powder was done by macerating 24 or 48 h using two levels of methanol, ethanol, or acetone as solvents (100%, 80%, and distilled water for the control). Evaluation of phenolic compounds, flavonoids, and antioxidant activity was performed. The resulting data were analyzed in SAS software version 9, and mean comparisons were performed by Duncan's Multi-Range Tests at the 1% and 5% probability levels. To determine the correlation between parameters, Pearson's correlation coefficient was checked using SPSS version 20 software.
Results: The results showed that there was a significant difference between 24 and 48 h of maceration to extract chemical compounds (P<0.01). The highest amounts of total phenol (24.75 mg GAE/g dw) and antioxidant activity (71.36%) were obtained in 80% acetone extract, and the maximum flavonoid content (6.03 mg Q/g dw) was achieved in 80% ethanol and 48 h maceration time. Generally, increasing the maceration time from 24 h to 48 h caused a significant rise in the extraction of phenolic compounds, flavonoids, and antioxidant activity. Moreover, there was a positive correlation between flavonoid content (P=0.86) and phenolic content (P=0.82) with antioxidant activity.
Discussion & Conclusion: According to the results, increasing the extraction time and also utilizing hydroalcoholic solvents were more effective in extracting these compounds than pure alcohols.

Keywords: Antioxidant, Flavonoid, Methanol, Acetone, Phenol, Digitalis purpurea L

Full-Text [PDF 841 kb] (443 Downloads)

Type of Study: Research | Subject: biochemistry
Received: 2023/07/7 | Accepted: 2023/11/8 | Published: 2024/02/4

References

1. Omid baigi R. Production and Processing of Medicinal Plants. 2nd ed. Astan Quds Publication. 2008; P. 304-6. (Persian)

2. Benli M, Yigit N, Geven F, Guney K, Bingol U. Antimicrobial activity of endemic Digitalis lamarckii Ivan from Turkey. Indian J Exp Biol 2009; 47: 218-21.

3. Kumaran A, Karunakaran RJ. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem 2006; 97:109-14. doi: 10.1016/j.foodchem.2005.03.032.

4. Milos H. Value-Function Approximations for Partially Observable Markov Decision Processes. JAIR 2011; 2000: 33-94. doi:10.48550/arXiv.1106.0234.

5. Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extract containing phenolic compounds. Food Chem 2006; 97: 654-60. doi: 10.1016/j.foodchem.2005.04.028.

6. Katanic J, Ceylan R, Matic S, Boroja T, Zengin G, Aktumsek A, et al. Novel perspectives on two Digitalis species: Phenolic profile, bioactivity, enzyme inhibition, and toxicological evaluation. S Afr J Bot 2017;109: 50-57. doi: 10.1016/j.sajb.2016.12.004.

7. Ahmadi F, Kadivar M, Shahedi M. Antioxidant activity of Kelussia odoratissima Moza in model and food systems. Food Chem 2007; 105: 57-64. doi: 10.1016/j.foodchem.2007.03.056.

8. Chirag P, Tyagi S, Halligudi N, Yadav J, Pathak S, Singh S, et al. Antioxidant activity of herbal plants: a recent review. JDDT 2013; 1: 1-8.

9. Ismail M, Ismail H I, Chan KW, Mariod AA. Phenolic content and antioxidant activity of Cantaloupe (Cucumis melo) methanolic extracts. Food Chem 2010; 119: 643-47. doi: 10.11648/j.ijnfs.20160501.13.

10. Jamshidi M, Ashtiani HR, Rezazade Sh, Fathiazad F, Mazandarani M, Khaki A. Evaluation and Comparison of phenolic compounds and antioxidant activity of some plant species in mazandaran. J Med Plants 2010; 9: 177-82. doi: 20.1001.1.2717204.2010.9.34.19.7.

11. Safi Z, Saeidi K, Lorigooini Z, Shirmardi HA. Evaluation of total phenols and antioxidant activity of Mullein (Verbascum songaricum) ecotypes. J Shahrekord Univ Med Sci 2016; 17: 68-75. (Persian)

12. Mathew S, Abraham TE. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food Chem 2006;44: 198-206. doi: 10.1016/j.fct.2005.06.013.

13. Pokorny J, Yanislieva N, Gordom M. Antioxidants in food-Practical Applications. Wood head Publishing Ltd and CRC Press LLC.2001; P. 140.

14. Rafaela GM, Gloria Lobo M, Monica G. The effect of extraction temperature, time and number of steps on the antioxidant capacity of methanolic banana peel extracts. Sep Purif Technol 2010; 71: 347-355. doi: 10.1016/j.seppur.2009.12.022.

15. Hayouni E, Abedrabba M, Bouix M, Hamdi M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chem 2007; 105:1126-34. doi: 10.1016/j.foodchem.2007.02.010.

16. Rezaie M, Farhoosh R, Iranshahi M, Sharif A, Golmohamadzadeh S. Ultrasonic-assisted extraction of antioxidative compounds from Bene (Pistacia atlantica subsp. mutica) hull using various solvents of different physicochemical properties. Food Chem 2015; 173:577-83. doi: 10.1016/j.foodchem.2014.10.081.

17. Kulisic T, Radonic A, Katalinic V, Milos M. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem 2004;85: 633-40. doi: 10.1016/j.foodchem.2003.07.024.

18. Jeevani MM, Bhat R, Karim AA. Effect of extraction solvents on the phenolic compounds and antioxidant activities of bunga kantan (Etlingera elatior Jack.) inflorescence. J Food Compos Anal 2011; 24:615-19. doi: 10.1016/j.jfca.2010.09.018.

19. Lin M, Tsai M, Wen K. Supercritical fluid extraction of flavonoids from Scutellaria Radix. J Chromatogr A 1999; 830: 387-95. doi:10.1016/S0021-9673(98)00906-6.

20. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their scavenging activity. Food Chem 2005; 91: 571-77. doi: 10.1016/j.foodchem.2004.10.006.

21. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. JFDA 2002; 10: 178-82. doi:10.38212/2224-6614.2748.

22. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT 1995; 28:25-30. doi:10.1016/S0023-6438 (95)80008-5.

23. Vilkhu K, Raymond M, Simons L, Bates D. Applications and opportunities for ultrasound assisted extraction in the food industry A review. IFSET 2007; 9: 161-69. doi: 10.1016/j.ifset.2007.04.014.

24. Nirmal SA, Patel AP, Bhawar SB, Pattan SR. Antihistaminic and antiallergic actions of extracts of Solanum nigrum berries: Possible role in the treatment of asthma. J Ethnopharmacol 2012; 142: 91-97. doi: 10.1016/j.jep.2012.04.019.

25. Davarynejad G, Taghizadeh S, Asili J. Effect of different solvents on total phenolic contents and antioxidant activity of Zizyphus jujube Miller fruits. J Hortic Sci 2017; 31: 158-66. doi: 10.22067/jhorts4.v0i0.47986.

26. Mohamadi M, Elhamirad AH, Pourfallah Z. Determination of total phenolic compound contents and antioxidant capacity of persimmon skin. Food Sci 2012; 2: 41-55. (Persian).

27. Liu Q, Yao H. Antioxidant activities of barley seeds extracts. Food Chem 2007; 102:732-37. doi: 10.12691/jfnr-7-2-7.

28. Medica-Saric M, Jasprica I, Smolcic-Bubalo A, Mornar A. Optimization of chromatographic conditions in thin layer chromatography of flavonoids and phenolic acids. Croat Chem Acta 2004; 77: 361-66.

29. Silva EM, Souza JNS, Rogez H, Rees JF, Larondelle Y. Antioxidant activities and polyphenolic contents of fifteen selected plant species from the Amazonian region. Food Chem 2007; 101:1012-18. doi: 10.1016/j.foodchem.2006.02.055.

30. Gharekhani M, Ghorbani M, Ebrahimzadeh MA, Jaafari SM, Sadeghi Mahoonak AR. Compare different methods of phenolic and flavonoid compounds extraction from Urtica dioica L. Iran J Med Aroma Plants 2010; 26: 389-405. (Persian).

31. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006; 1142-45.

32. Masoko D, GaloloS S, Mokgotho MP, Eloff JN, Howard Rl, Mampara LJ. Evaluation of the antioxidant, antibacterial and antiproliferative activities of the acetone extract of the roots of sennaitulical (fabaceue). JTCM 2010; 7:138-48. doi:10.4314/AJTCAM.V7I2.50873.

33. Ghasemi K, Ghasemi Y, Ebrahimzadeh MA. Antioxidant activity, phenol and flavonoid contents of 13 Citrus specis peels and tissues. Pak J Pharm Sci 2009; 22: 277-81.

34. Mandal V, Mohan Y, Hemalatha S. Microwave assisted extraction, an innovative and promising extraction tool for medicinal plant research. Phcog Rev 2007; 1: 8-14.

35. Kamkar A, Jebelli Javan A, Asadi F, Kamalinejad M. The antioxidative effect of Iranian Mentha pulegium extracts and essential oil in sunflower oil. Food Chem Toxicol 2010; 48: 1796-800. doi: 10.1016/j.fct.2010.04.003.

36. Khanavi M, Hajimahmoodi M, Cheraghi-Niroomand M, Kargar Z, Ajani Y, Hadjiakhoondi A, et al. Comparison of the antioxidant activity and total phenolic contents in some Stachys species. Afr J Biotechnol 2009; 8:1143-47.

37. Locatelli M, Travaglia F, Coisson JD, Martelli A, Stevigny C, Arlorio M. Analytical methods total antioxidant activity of hazelnut skin (NocciolaPiemonte PGI): Impact of different roasting conditions. Food Chem 2010;119: 1647-55. doi: 10.1016/J.FOODCHEM.2009.08.048.

38. Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confusa bark and heartwood. J Agric Food Chem 2001; 49; 3420-24. doi: 10.1021/jf0100907.

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Rezaie allolo A, SaniKhani M, Khairy A A, Yaghoobi M. Effect of maceration time and type of solvent on the content of phenolic compounds and antioxidant activity of Digitalis purpurea L. J. Ilam Uni. Med. Sci. 2024; 31 (6) :11-22
URL: http://sjimu.medilam.ac.ir/article-1-8029-en.html

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