[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations65903843
h-index2721
i10-index19688

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 32, Issue 3 (8-2024) ::
Journal of Ilam University of Medical Sciences 2024, 32(3): 21-34 Back to browse issues page
Investigating the inhibitory activity of iron oxide nanoparticles attached to coumarin on breast cancer cell line
Zahra Ghavamifard1 , Abdolhassan Doulah * 2, Ali Salehzadeh3
1- Dept of Biology, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
2- Dept of Biology, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran , h_doulah@yahoo.com
3- Dept of Biology, Rasht Branch, Islamic Azad University, Rasht,
Abstract:   (97 Views)
Introduction:  The use of functionalized metal nanoparticles against cancer cells has gained assiduous attention. This study aimed to assess the inhibitory activity of iron oxide nanoparticles coated with glucose and conjugated with coumarin (Fe3O4@Glu-Coumarin) on breast cancer cell line, as well as the expression of the caspase-8 and caspase-9 genes.
Material & Methods: The physical and chemical properties of nanoparticles were evaluated using FT-IR, XRD, EDS-map, and electron microscope imaging. The toxicity of the synthesized nanoparticles was determined using the MTT assay, and the 50% inhibitory dose (IC50) was calculated. Moreover, the effect of nanoparticles on apoptosis induction was investigated by measuring the expression level of the caspase 8 and 9 genes and flow cytometry analysis. Statistical analyses were performed in SPSS software. One-way analysis of variance (ANOVA) was employed to assess significant differences between nanoparticle-treated and control groups. A p-value of less than 0.05 was regarded as statistically significant.
Results: The synthesis of Fe3O4@Glu-Coumarin nanoparticles was confirmed by physicochemical tests, including FT-IR, XRD, EDS-mapping, and electron microscope imaging. The nanoparticles had dimensions of 25 to 50 nm and contained Fe, O, and C elements. The treatment of MCF-7 cells with nanoparticles caused a significant decrease in the survival of cancer cells, and the IC50 was 93μg/ml. The exposure of cells to the nanoparticles caused a marked increase in the expression of caspases 8 and 9 by 2.6 and 2.9 folds, respectively, compared to the control group. In addition, the frequency of apoptotic cells after treatment with the nanoparticles increased from 2.21% to 84%.
Discussion & Conclusion: The results of this study pointed out that Fe3O4@Glu-Coumarin increased the expression of the caspases 8 and 9 genes in breast cancer cells and, as a result, can activate the intrinsic and extrinsic apoptotic pathways, thereby inhibiting the proliferation of cancer cells.
Keywords: Apoptosis, Breast cancer, Coumarin, Flow cytometry
Full-Text [PDF 1455 kb]   (40 Downloads)    
Type of Study: Research | Subject: Molecular Genetics
Received: 2023/05/26 | Accepted: 2024/03/9 | Published: 2024/08/5
References
1. Farzin A, Etesami SA, Quint J, Memic A, Tamayol A. Magnetic nanoparticles in cancer therapy and diagnosis. Adv Healthc Mater 2020; 9:1901058. doi: 10.1002/adhm.201901058.
2. Küpeli Akkol E, Genç Y, Karpuz B, Sobarzo-Sánchez E, Capasso R. Coumarins and coumarin-related compounds in pharmacotherapy of cancer. Cancers 2020; 12:1959. doi: 10.3390/cancers12071959.
3. Sandhu S, Bansal Y, Silakari O, Bansal G. Coumarin hybrids as novel therapeutic agents. Bioorg Med Chem 2014; 22: 3806-14. doi:10.1016/j.bmc.2014.05.032.
4. Stanchev S, Momekov G, Jensen F, Manolov I. Synthesis, computational study and cytotoxic activity of new 4-hydroxycoumarin derivatives. Eur J Med Chem 2008; 43:694-706. doi: 10.1016/j.ejmech.2007.05.005.
5. Xiao CF, Tao LY, Sun HY, Wei W, Chen Y, Fu LW, et al. Design, synthesis and antitumor activity of a series of novel coumarin–stilbenes hybrids, the 3-arylcoumarins. Chin Chem Lett 2010; 21:1295-8. doi: 10.1016/j.cclet.2010.04.034.
6. Amin KM, Eissa AA, Abou-Seri SM, Awadallah FM, Hassan GS. Synthesis and biological evaluation of novel coumarin–pyrazoline hybrids endowed with phenylsulfonyl moiety as antitumor agents. Eur J Med Chem 2013; 60: 187-98. doi: 10.1016/j.ejmech.2012.12.004.
7. Shokrollahi F, Salehzadeh A, Kafilzadeh F, Zaefizadeh M. Evaluation the effect of Iron oxide nanoparticles functionalized by glucose and conjugated with Coumarin (Fe3O4@ Glu-coumarin NPs) on expression of CASP8, CASP9, p53, mTOR1, and MAPK1 genes in liver cancer cell line. Gene Rep 2023; 33: 101818. doi:10.1016/j.genrep.2023.101818.
8. Kumari R, Saini AK, Kumar A, Saini RV. Apoptosis induction in lung and prostate cancer cells through silver nanoparticles synthesized from Pinus roxburghii bioactive fraction. JBIC J Biol Inorg Chem 2020; 25:23-37. doi:10.1007/s00775-019-01729-3.
9. Pfaffl MW. A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Res 2001; 29:e45. doi:10.1093/nar/29.9.e45.
10. Yang J, Liu X, Bhalla K, Kim CN, Ibrado AM, Cai J, et al. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked. Science 1997;275:1129-32. doi: 10.1126/science.275.5303.112.
11. Ohren JF, Chen H, Pavlovsky A, Whitehead C, Zhang E, Kuffa P, et al. Structures of human MAP kinase kinase 1 (MEK1) and MEK2 describe novel noncompetitive kinase inhibition. Nat Struct Mol Biol 2004; 11:1192-7. doi:10.1038/nsmb859.
12. Goel A, Prasad AK, Parmar VS, Ghosh B, Saini N. 7, 8-Dihydroxy-4-methylcoumarin induces apoptosis of human lung adenocarcinoma cells by ROS-independent mitochondrial pathway through partial inhibition of ERK/MAPK signaling. FEBS Lett 2007;581:2447-54. doi: 10.1016/j.febslet.2007.04.052.
13. Cui N, Lin DD, Shen Y, Shi JG, Wang B, Zhao MZ, et al. Triphenylethylene-Coumarin Hybrid TCH-5c Suppresses Tumorigenic Progression in Breast Cancer Mainly Through the Inhibition of Angiogenesis. Anti-Cancer Agents Med Chem 2019; 19:1253-61. doi: 10.2174/1871520619666190404155230.
14. Khan MI, Mohammad A, Patil G, Naqvi SA, Chauhan LK, Ahmad I. Induction of ROS, mitochondrial damage and autophagy in lung epithelial cancer cells by iron oxide nanoparticles. Biomaterials 2012; 33:1477-88. doi:10.1016/j.biomaterials.2011.10.080.
15. Jaffri SA, Yan Y, Schwirz J, Schetelig MF. Functional characterization of the Drosophila suzukii pro-apoptotic genes reaper, head involution defective and grim. Apoptosis 2020; 25: 864-74. doi: 10.1007/s10495-020-01640-2.
16. Okamoto T, Suzuki T, Kusakabe S, Tokunaga M, Hirano J, Miyata Y, et al. Regulation of apoptosis during flavivirus infection. Viruses 2017; 9:243. doi:10.3390/v9090243.
17. Bigdeli R, Shahnazari M, Panahnejad E, Cohan RA, Dashbolaghi A, Asgary V. Cytotoxic and apoptotic properties of silver chloride nanoparticles synthesized using Escherichia coli cell-free supernatant on human breast cancer MCF 7 cell line. Artif Cells Nanomed Biotechnol 2019; 47:1603-9. doi:10.1080/21691401.2019.1604533.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghavamifard Z, Doulah A, Salehzadeh A. Investigating the inhibitory activity of iron oxide nanoparticles attached to coumarin on breast cancer cell line. J. Ilam Uni. Med. Sci. 2024; 32 (3) :21-34
URL: http://sjimu.medilam.ac.ir/article-1-7989-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 32, Issue 3 (8-2024) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.15 seconds with 41 queries by YEKTAWEB 4657