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:: Volume 30, Issue 5 (12-2022) ::
Journal of Ilam University of Medical Sciences 2022, 30(5): 40-50 Back to browse issues page
Effects of Biosynthesized Zinc and Copper Nanoparticles on Medicine Resistant Pathogenic Bacteria(Antibiotics)
Yasman sadat Nabipour1 , Arman Rostamzad * 2, Ardeshir Hesampour1 , Maryam Tajabadi1 , Salman Ahmadi asbghin3
1- Dept of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Dept of Biology, Faculty of Basic Sciences, Ilam University, Ilam, Iran , a.rostamzad@ilam.ac.ir
3- Dept of Biology, Faculty of Basic Sciences, Mazandaran University, Mazandaran, Iran
Abstract:   (153 Views)
Introduction: In this study, the antimicrobial activity of biologically synthesized zinc (Zn) and copper (Cu) nanoparticles was investigated on gram-positive and gram-negative bacteria, pathogens, and resistant and common nosocomial infections.
Material & Methods: Intially, zinc oxide and copper nanoparticles were synthesized using Xanthomonas campestris and Pseudomonas stutzeri bacteria, respectively. To investigate the effect of different concentrations of nanoparticles on bacteria using the macrodilution method, concentrations of 0.01, 0.1, 0.5, 1, and 1.5% of Zn and Cu nanoparticles (culture medium + nanoparticles) were prepared and were added to the respective bacteria at a concentration of 105 cell/ml. Containers containing treated media (bacteria + nanoparticles) and control media were placed in a shaker incubator. Afterward, the optical density (OD) of treatment and positive control and negative control media were determined.
(Ethic code: IR.ILAM.REC.1401.008)
Findings: The results of statistical analysis showed that Zn and Cu nanoparticles, at a concentration of 0.5%, were able to remove almost all (100%) Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus bacteria and were bacteriostatic at the concentration of 0.1%.
Discussion & Conclusion: The results obtained from the determination of antibacterial properties of nanoparticles showed a direct relationship between the concentration of nanoparticles and the percentage of bacterial removal.
 
Keywords: Acinetobacter baumannii, Bacteriostatic, Klebsiella pneumoniae, Macrodilution, Nanoparticle, Pseudomonas stutzeri, Xanthomonas campestris, Staphylococcus aureus
Full-Text [PDF 1233 kb]   (75 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2021/09/9 | Accepted: 2022/05/16 | Published: 2022/12/6
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Nabipour Y S, Rostamzad A, Hesampour A, tajabadi M, Ahmadi asbghin S. Effects of Biosynthesized Zinc and Copper Nanoparticles on Medicine Resistant Pathogenic Bacteria(Antibiotics). sjimu 2022; 30 (5) :40-50
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Volume 30, Issue 5 (12-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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