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:: Volume 30, Issue 4 (10-2022) ::
Journal of Ilam University of Medical Sciences 2022, 30(4): 39-46 Back to browse issues page
Effect of Aspartame on Histology and Histomorphometry of Stomach in Balb/C Mice
Zahra Tootian * 1, Simin Fazelipour2 , Mohammad taghi Sheibani3 , Hossien Erik-Aghaji4 , Reyhaneh Hooshmand Abbasi3
1- Dept of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran , ztootian@yahoo.com
2- Dept of Anatomy, Faculty of Medicine, Tehran Medical Branch, Islamic Azad University, Tehran, Iran
3- Dept of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4- Dept of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:   (1172 Views)
Introduction: Aspartame is one of the synthetic sweeteners widely used in the food industry as a sugar substitute in recent decades. The present study aimed to investigate the effect of different doses of aspartame on histological and histomorphometric changes in the stomach in BALB/C mice.
Material & Methods: In this study, 24 BALB/C mice aged three weeks were selected and divided into three experimental groups that received 0.3 ml aspartame solution at doses of 100, 200, and 400 mg/kg body weight, and a control group that received drinking water with the same condition up to nine weeks of age. Eventually, some tissue sections were prepared from the stomachs and stained using the hematoxylin-eosin method. After histological evaluation, the necessary images were prepared and the histomorphometric examination was conducted using an optical microscope equipped with Axiovision software. The thickness of mucosa, submucosa, musculature, and depth of pits was measured and the frequency of parietal cells was calculated in the dimensions of 6.25 × 104 μm2.
(Ethic code: 7506001/6/7)  
Findings: Histological results indicated destruction and disruption of the mucosal epithelium and gastric pits and atrophy of gastric glands including glandular cells. In the histomorphometric examination of the non-glandular part, only the thickness of the mucosa had a significant difference in the group receiving the highest dose of aspartame compared to the control group (P<0.05). However, in the evaluation of the glandular part, mucosal and muscle thickness in all three experimental groups and the submucosa thickness in the glandular and non-glandular parts in the experimental group (400 mg body weight) showed a significant increase compared to the control group (P<0.05). Moreover, the depth of gastric pits increased significantly and the frequency of parietal cells had a significant decrease in the experimental groups compared to the control group (P<0.05).
Discussion & Conclusion: Although aspartame may partially play a protective role by decreasing parietal cells, it should be noted that higher doses of aspartame could induce tissue changes in different layers of the stomach. Therefore, it is recommended to use it with more caution.

Keywords: Aspartame, Histology, Histomorphometry, Mice, Stomach
Full-Text [PDF 1018 kb]   (461 Downloads)    
Type of Study: Research | Subject: anatomy
Received: 2021/08/12 | Accepted: 2022/04/20 | Published: 2022/10/7
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Tootian Z, Fazelipour S, Sheibani M T, Erik-Aghaji H, Hooshmand Abbasi R. Effect of Aspartame on Histology and Histomorphometry of Stomach in Balb/C Mice. J. Ilam Uni. Med. Sci. 2022; 30 (4) :39-46
URL: http://sjimu.medilam.ac.ir/article-1-7222-en.html


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Volume 30, Issue 4 (10-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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