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:: Volume 29, Issue 5 (11-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(5): 44-55 Back to browse issues page
Bacteriolytic Activity of Novel Bacteriophage PϕBw Ec01 from Cystoviridae Family against the Clinical Strain of Antibiotic Resistant Escherichia Coli in Burn Wounds
Ladan Rahimzadeh Torabi1 , Monir Doudi * 2, Nafiseh sadat Naghavi1, Ramesh Monajemi3
1- Dept of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, Iran
2- Dept of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, Iran , monirdoudi@yahoo.com
3- Dept of Biology, Islamic Azad University, Falavarjan Branch, Isfahan, Iran
Abstract:   (1049 Views)
Introduction:The emergence of Escherichia coli resistance to common antibiotics in burn wound patients has become a controversial problem in Iran trauma and burn hospitals. The emergence of more and more drug resistance by infectious pathogens paves the way for further study of the nature of phages, and phage therapy can significantly address this crisis. In the first phase, this study aimed to isolate and identify the biochemical and molecular nature of antibiotic-resistant E. coli, which causes burn wound infections. The second phase of this study isolated the specific phages of the bacteria in these wounds and then evaluated the morphological characteristics of the phage and the host area.
Material & Methods: In this study, 50 bacterial strains were isolated from specialized accident and burn hospitals in Isfahan, Yazd, Tehran, and Rasht. Accurate identification and study of antibiotic resistance profile was performed by disk diffusion method on agar. The 16S rRNA coding gene was amplified using the PCR technique. The PCR product was then sent to "Gene Azma" laboratory for sequencing. In order to isolate the possible phages, a sample was taken from the raw wastewater (entrance of the northern treatment plant) in Isfahan, Iran. Phage morphology was assessed and reported by transmission electron microscopy (TEM); moreover, phage plate count and host range were assessed for this bacteriophage.
Findings: Bacterial 16S rRNA sequence was located in NCBI with MW844043 Accession Number. The specific phage PϕBw-Ec01 was significantly able to infect resistant E. coli bacteria. TEM demonstrated that the isolated phage was dsRNA and belonged to the family Cystoviridae with prototype ɸ6. PϕBw-Ec01 lytic phage was highly effective in inhibiting the growth of E. coli strain ADB_66-1 in this study.
Discussion & Conclusion: The results of this report showed that E. coli isolated from burn wounds of hospitalized patients had high resistance to common antibiotics. The studied phage in this study can be a good choice and a suitable option for controlling and inhibiting these resistant pathogens in the burn wounds of hospitalized patients. It is hoped that with more extensive research on the identity and study of the effectiveness of phages, the rate of bacterial lysis will be investigated. This issue will reduce the microbial load caused by resistant and infectious pathogens and can be used as an effective adjuvant against burn wound infections.
Keywords: Antibiotic resistance, Bacteriophage, Burn wound infection, Escherichia coli, Phage therapy, 16S rRNA
Full-Text [PDF 856 kb]   (320 Downloads)    
Type of Study: Research | Subject: Medical microbiology
Received: 2021/05/18 | Accepted: 2021/06/26 | Published: 2021/11/8
References
1. Nikaido H. Multidrug resistance in bacteria. Annu Rev Biochem 2009; 78:119-46. doi.10.1146/annurev.biochem.78.082907.145923
2. Li XZ, Plesiat P, Nikaido H. The challenge of efflux mediated antibiotic resistance in Gram negative bacteria. Clin Microbiol Rev 2015; 28:337-418. doi. 10.1128/CMR.00117-14
3. Richardson LA. Understanding and overcoming antibiotic resistance. PLos Biol2017; 15: 2003775. doi.10.1371/journal.pbio.2003775.
4. Summer GJ, Puntillo KA, Miaskowski C, Green PG, Levine JD. Burn injury pain the continuing challenge. J Pai 2007; 8:533-48. doi.10.1016/ j.jpain.2007.02.426.
5. Rosenkranz KM, Sheridan R. Management of the burned trauma patient balancing conflicting priorities. Burns 2002; 28:665-9. doi.10.1016/ s0305-4179(02)00109-2.
6. Hawkins A, Maclennan PA, Mcgwin JRG, Cross JM, Rue LW. The impact of combined trauma and burns on patient mortality. J Trauma 2005; 58:284-8. doi.10.1097/01.ta.0000130610.19361. bd.
7. Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev 2001; 14:244-69. doi.10.1128/CMR.14.2.244-269.2001.
8. Loccarrillo C, Wu S, Beck JP. Phage therapy of wounds and related purulent infections. 1th ed. Cambridge USA CAB Int Publication. 2012; P.185-202.
9. Centers for disease control and prevention. Antibiotic antimicrobial resistance. 2 th ed. CDC Gov Drug Publication. 2017; P.1-100.
10. Church D, Elsayed S, Reid O, Winston B, Lind‌say R. Burn wound infections. Clin Microbiol Rev 2006; 19:403-34. doi.10.1128/CMR.19.2.403-434.2006.
11. Mayhall CG. The epidemiology of burn wound infections then and now. Clin Infect Dis 2003; 37543-50. doi.10.1086/376993.
12. Rafla K, Tredget EE. Infection control in the burn unit. Burns 2011; 37:5-15. doi. 10.1016/j.burns.
13. Azzopardi EA, Azzopardi E, Camilleri L, Villapalos J, Boyce DE, Dziewulski P, et al. Gram negative wound infection in hospitalised adult burn patients systematic review and metanalysis. Plos One 2014; 9:95042. doi.10.1371/journal.pone.0095042.
14. Norbury W, Herndon DN, Tanksley J, Jeschke MG, Finnerty CC. Infection in Burns. Surg Inf 2016; 17:250-5. doi.10.1089/sur.2013.134.
15. Chan Benjamin K, Abedonstephen T, Loccarrillo C. Phage cocktails and the future of phage therapy. Future Microbiol2013; 8:769-83. doi.10.2217/ fmb.13.47.
16. Doss J, Culbertson K, Hahn D, Camacho J, Barekzi N. A review of phage therapy against bacterial pathogens of aquatic and terrestrial organisms. Viruses 2017; 9:50. doi.10.3390/v9030050
17. Rahimzadehtorabi L, Doudi M, Noori A. [Antibacterial effects of gold nanoparticles on multi sdrug resistant Klebsiella pneumoniae and Escherichia coli and Its effect on the liver of balb/c Mice]. JSSU2016; 23:1001-17. (Persian)
18. Goli HR, Nahaei MR, Ahangarzadehrezaee M. Emergence of colistin resistant Pseudomonas aeruginosa at Tabriz hospitals Iran. Iran J Microbiol 2016; 8:62-9.
19. Weisburg WG, Barns SM, Pelletier DA et al. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697-703. doi.10.1128/ jb.173.2.697-703.1991.
20. Naghavi NS, Golgoljam M, Akbari, M. Effect of three sewage isolated bacteriophages on the multi drug resistant pathogenic bacteria. J Biol Sci 2013. doi.10.3923/jbs.2013.422.426
21. Ghasemi SM, Bouzari M, Emtiazi G. Preliminary characterization of Lactococcus garvieae bacteriophage isolated from wastewater as a potential agent for biological control of lactococcosis in aquaculture. Aquacult Int 2014; 22:1469-80. doi.10.1007/s00705-014-2142-z.
22. Rahimzadehtorabi L, Doudi M, Naghavi N, Monajemi R. Isolation characterization, and effectiveness of bacteriophage against XDR Acinetobacter baumannii isolated from nosocomial burn wound infection. Iranian J Bas Med Sci 2021; 24: 1254-63. doi.10.22038/ijbms.2021.57772.12850.
23. Mendes JJ, Leandro C, Cortereal S. Wound healing potential of topical bacteriophage therapy on diabetic cutaneous wounds. Wound Rep Reg 2013; 21:595-603. doi.10.1111/wrr.12056.
24. Rahimzadeh Torabi L, Doudi M, Naghavi N, Monajemi R. Bacteriophages pen-cl and pen-ho can eliminate MDR Enterobacter cloacae and Enterobacter hormaechei isolated from burn wound infections without toxicity for human skin cells. FEMS Microbiol Let 2021; 368: 143. doi.10.1093/femsle/fnab143.
25. Rahimzadehtorabi L, Naghavi NS, Doudi M, Monajemi R. Efficacious antibacterial potency of novel bacteriophages against ESBL producing Klebsiella pneumoniae isolated from burn wound infections. Iran J Microbiol 2021; 13:678-690. doi.10.18502/ijm. v13i5.7435.
26. Shokri D, Soleimani DA, Moayednia R, Mobasherizadeh S, Shirsalimian M, Enayatollahi S, et al. Isolation identification and evaluation of two lytic Bacteriophages against clinical antibiotic-resistant strains of Pseudomonas aeruginosa from waste water and hospital sewage of Isfahan city. Sci J Ilam Uni Med Scie 2015; 23:164-1172.
27. Zare L, Shenagari M, Khan Mirzaei MA, Mojtahedi A. Isolation of lytic phages against pathogenic E. coli isolated from diabtic ulcers. Iran J Med Microbiol 2017; 11:34-41.
28. Rahmani R, Zarrini G, Sheikhzadeh F, Aghamohammadzadeh N. Effective phages as green antimicrobial agents against antibiotic resistant hospital Escherichia coli Jundishapur J Microbiol 2015; 8:59808. doi.10.5812/jjm.17744. doi.10.5812/jjm.17744.
29. Hosainzadegan H, Mohammadi M, Pajohy N, Ebrahimzade F. Study of lytic phage and alkaline phosphatase on treatment of burn infections caused by Escherichia coli in MouseIran J Med Microbiol 2008; 2:37-43.
30. Servick K. Beleaguered phage therapy trial presses on. Science 2016; 352:1506. doi.10.1126/science.
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Rahimzadeh Torabi L, Doudi M, Naghavi N S, Monajemi R. Bacteriolytic Activity of Novel Bacteriophage PϕBw Ec01 from Cystoviridae Family against the Clinical Strain of Antibiotic Resistant Escherichia Coli in Burn Wounds. Journal of Ilam University of Medical Sciences 2021; 29 (5) :44-55
URL: http://sjimu.medilam.ac.ir/article-1-7099-en.html


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Volume 29, Issue 5 (11-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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