[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019

Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
Registered in



:: Volume 29, Issue 5 (12-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(5): 89-101 Back to browse issues page
Synthesis of PEG-PLA Block Copolymer Nanospheres Containing Mixed Recombinant Proteins of Protective Antigen and Lethal Factor of Bacillus anthracis
Hossein Honari * 1, Mohammad Ebrahim Minaei2 , Hassan Mirhaj2 , Sayyed Masih Etemadaubi2
1- Dept of Biology, Faculty of Basic Sciences, Imam Hossein Comprehension University, Tehran, Iran , Honari.hosein@gmail.com
2- Dept of Biology, Faculty of Basic Sciences, Imam Hossein Comprehension University, Tehran, Iran
Abstract:   (1156 Views)
Introduction: Anthrax is a zoonotic disease and vaccine production is one way to protect people against this bacterium. This study investigates the application of copolymer nanocapsule (PEG-PLA) systems with controlled release specificity for a recombinant protective antigen and lethal factor of Bacillus anthracis to provide a vaccine candidate.
Material & Methods: In this experimental study, the dual solvent evaporation emulsion method was used to produce nanocapsules. Moreover, zeta potential and size of nanoparticles, loading efficiency of nanoparticles, recombinant protein release pattern, the probable effect of PLA-PEG nanoparticle production on the shelf life of recombinant proteins were investigated in this study. Mice were used as test and control samples for antibody production and immune response evaluation.
(Ethic code: 9727250)
Findings: The results of this study showed that mixed proteins (LFD1+PAD4) loaded on PEG-PLA block copolymer had an average size of 109 nm, the zeta potential of -27.7 mV, and PDI=0.394. In this study, it was shown that the release of these antigens was carried out in two stages of rapid and slow release. The release of the proteins was estimated at about 20% on the first day and 78% on the 49th day. The titers of antibodies produced in the serum of the mouse groups against these antigens at defined intervals were significantly different from each other.
Discussion & Conclusion: The results of this study suggest the application of PEG-PLA block copolymer nanocapsules containing mixed recombinant proteins of protective antigen and lethal factor of Bacillus anthracis. Due to less degradation and more protection of antigen activity in nanoparticles, compared to traditional methods, as well as fewer repeat injections, higher specificity, reduced side effects, lower cost, and slower release rate, the use of nanoparticles is a good option to replace traditional methods.
Keywords: Copolymer nanocapsule, Lethal factor, Protective antigen, Vaccine
Full-Text [PDF 816 kb]   (635 Downloads)    
Type of Study: Research | Subject: Molecular Genetics
Received: 2020/11/25 | Accepted: 2021/09/1 | Published: 2021/11/8
1. Riedel S, Hobden JA, Miller S, Morse SA, Mietzner TA, Detrick B, et al. Jawetz melnick adelbergs medical microbiology. 28th ed. Mcgraw Hill Publication. 2007; P.231-6.
2. Khardori N. Anthrax bacteriology clinical presentations and management in khardori bioterrorism preparedness medicine public health policy. Wiley VCH Verlag GmbH Weinheim Publication.2006; P. 123-45.
3. Gonzalez MR, Bischofberger M, Pernot L, Goot FG, Freche B. Bacterial poreforming toxins the hole story. Cell Mol Life Sci 2008; 65:493-507
4. Edwards KA, Clancy HA, Baeumner AJ. Bacillus anthracis toxicology, epidemiology and current rapid detection methods. Anal Bioanal Chem2006;384:73-84. doi.10.1007/s00216-005-0090-x.
5. Gupta P, Waheed SM, Bhatnagar R. Expression and purification of the recombinant protective antigen of Bacillus anthracis. Prote Exp Pur 1999; 16:369-76. doi.10.1006/prep.1999.1066.
6. Wang, JY, Roehrl, MH. Anthrax vaccine design: strategies to achieve comprehensive protection against spore Bacillus and toxin. Med Immunol2005; 24: 4:4. doi.10.1186/1476-9433-4-4
7. Hepburn MJ, Hugh Dyson E, Simpson AJ, Brenneman KE, Bailey N, Wilkinson L, Hornby R, Mateczun AJ, Bell MG, Baillie LW. Immune response to two different dosing schedules of the anthrax vaccine precipitated vaccine. Vaccine2007; 25:6089-97. doi.10.1016/j.vaccine.2007.05.018.
8. Chi X, Li J, Liu W, Wang X, Yin K, Liu J, Zhang X. Generation and characterization of human monoclonal antibodies targeting anthrax protective antigen following vaccination with a recombinant protective antigen vaccine. Clin Vac Immunol 2015; 22: 553-60. doi.10.1128/CVI.00792-14
9. Williamson D, Dyson EH. Anthrax prophylaxis recent advances and future directions. Front Microbiol2015; 6, 1009.‌ doi.10.3389/fmicb.2015.01009
10. Knockenhauer KE, Sawicka KM, Roemer EJ, Simon SR. Protective antigen composite nanofibers as a transdermal anthrax vaccine. Con Proc IEEE Eng Med Biol Soc2008;5: 1040-3. doi. 10.1109/IEMBS.2008.4649337
11. Blum JS, Saltzman WM. High loading efficiency and tunable release of plasmid DNA encapsulated in submicron particles fabricated from Plga conjugated with poly L lysine]. J Cont Rel 2008;129:66-72. doi.10.1016/j.jconrel.2008.04.002
12. Ghasemi R, Abdollahi M, Zadeh EE, Khodabakhshi K, Badeli A, Bagheri H, et al. Mpeg-pla and pla-peg-pla nanoparticles as new carriers for delivery of recombinant human growth hormone. Sci Rep2018;8:9854. doi.10.1038/s41598-018-28092-8
13. Etemadaubi M, Honari H, Hajinourmohamadi A, Bagheri H, Noofeli M. [Assessment of cytotoxicity of Bacillus anthracis recombinant protective antigen in free and encapsulated forms by double block Pla-peg and Pcl-peg copolymers on vero cell]. Jundishapur Sci Med J 2018; 17:377-86. (Persian)
14. Singh NA, Mandal AKA, Khan ZA. Fabrication of Pla-peg nanoparticles as delivery systems for improved stability and controlled release of catechin. J Nanomate 2017; :1-9. doi. 10.1155/2017/6907149
15. Kim SK, Foote MB, Huang L. The targeted intracellular delivery of cytochrome C protein to tumors using lipid apolipoprotein nanoparticles]. Biomaterials2012;33:3959-66. doi. 10.1016/j.biomaterials.2012.02.010
16. Park J, Wrzesinski SH, Stern E, Look M, Criscione J, Ragheb R, et al. Combination delivery of TGFβ inhibitor and IL2 by nanoscale liposomal polymeric gels enhances tumour immunotherapy. Nat Mate 2012;11:895-905. doi.10.1038/nmat3355
17. Manickavasagam D, Novak K, Oyewumi MO. Therapeutic delivery of simvastatin loaded in Pla-peg polymersomes resulted in amplification of anti inflammatory effects in activated microglia. AAPS J2017;20:18. doi.10.1208/s12248-017-0176-3
18. Vila A, Gill H, Mccallion O, Alonso MJ. Transport of Pla-peg particles across the nasal mucosa effect of particle size and peg coating density]. J Cont Rel2004;98:231-44. doi.10.1016/j.jconrel.2004.04.026
19. Manish M, Rahi A, Kaur M, Bhatnagar R, Singh S. A single dose Plga encapsulated protective antigen domain 4 nanoformulation protects Mice against Bacillus anthracis spore challenge. Plos One 2013;8:61885. doi.10.1371/journal.pone.0061885
20. Malik A, Gupta M, Mani R, Gogoi H, Bhatnagar R. Trimethyl chitosan nanoparticles encapsulated Protective antigen protects the Mice against anthrax. Front Immunol. 2018;9:562. doi. 10.3389/fimmu.2018.00562
21. Jain AK, Goyal AK, Mishra N, Vaidya B, Mangal S, Vyas SP. Peg-pla–peg block copolymeric nanoparticles for oral immunization against hepatitis B. Int J Pharm 2010;387:253-62. doi.10.1016/j.ijpharm.2009.12.013
22. Dong Y, Feng SS. Nanoparticles of poly D L‐lactide methoxy poly ethylene glycol poly D L lactide blends for controlled release of paclitaxel. J Biomed Mate Res2006;78:9-12. doi. 10.1002/jbm.a.30684
23. Li M, Cai RJ, Song S, Jiang ZY, Li Y, Gou HC, Qiu HJ. Evaluation of immunogenicity and protective efficacy of recombinant outer membrane proteins of Haemophilus parasuis serovar 5 in a murine model. PLos One 2017;12:176537. doi.10.1371/journal.pone.0176537
24. Martins VT, Chavezfumagalli MA, Lage DP, Duarte MC, Garde E, Costa LE. et al. Antigenicity immunogenicity and protective efficacy of three proteins expressed in the promastigote and amastigote stages of Leishmania infantum against visceral Leishmaniasis. PLos One2015;10:137683. doi. 10.1371/journal.pone.0137683
Send email to the article author

Add your comments about this article
Your username or Email:


Ethics code: نیاز ندارد

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Honari H, Minaei M E, Mirhaj H, Etemadaubi S M. Synthesis of PEG-PLA Block Copolymer Nanospheres Containing Mixed Recombinant Proteins of Protective Antigen and Lethal Factor of Bacillus anthracis. J. Ilam Uni. Med. Sci. 2021; 29 (5) :89-101
URL: http://sjimu.medilam.ac.ir/article-1-6867-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 29, Issue 5 (12-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.15 seconds with 41 queries by YEKTAWEB 4646