[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 29, Issue 1 (3-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(1): 65-78 Back to browse issues page
Phytochemical Study and Comparison of Antioxidant Potential and Phenolic Content of Different Solvent Extract of Flaxseed (Linum usitatissimum L.) and Biosynthesis of Gold and Silver Nanoparticles using its Extract
Roya Mirzajani * 1, Maryam Kolahi2 , Fatemeh Gorgian3
1- Dept of Chemistry, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran , rmirzajani@scu.ac.ir
2- Dept of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3- Dept of Chemistry, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (1502 Views)
Introduction: Flaxseed (Linum usitatissimum L.) is a member of the Lamiaceae family used in nutrition, health, and industrial products. Flaxseed accumulates many biologically active compounds, including linolenic acid, linoleic acid, lignans, cyclic peptides, polysaccharides, alkaloids, and cyanogenic glycosides. Recent research suggests that flaxseed lowers cholesterol, stabilizes blood sugar, prevents osteoporosis, helps with weight loss, strengthens the immune system, and prevents cancer. This study aimed to investigate the phytochemical, antioxidant, and phenolic content of extracts of various flaxseed solvents and the biosynthesis of silver and gold nanoparticles using these extracts.
 
Materials & Methods: In order to evaluate the antioxidant power and phenolic content of flaxseeds, flaxseeds extract was extracted using three different solvents, namely ethyl acetate, dichloromethane, and normal hexane by the soxhlet method. Phytochemical tests were used to identify chemical compounds. Following that, for the synthesis of gold and silver nanoparticles, HAuCl4, 3H2O, and AgNO3 salts, as well as dichloromethane extract were used in descending order. Moreover, the characterization of synthesized nanoparticles was performed by various techniques, including UltraViolet-Vis spectrophotometry. Furthermore, transmission electron microscopy and nanoparticle size analysis techniques were used to examine the size and distribution extent of nanoparticles.
Ethics code: EE/1400.3.02.4678 /scu.ac.ir
 
Findings: After extracting different extracts of flaxseed, various phytochemical tests and different reagents were used to qualitatively identify the secondary metabolites in the extract. The results showed the presence of flavonoids, alkaloids, and steroids, as well as a lack of tannins and saponins. The highest and lowest phenolic compounds were related to the extract of dichloromethane and n-hexane of flaxseeds, respectively. The highest antioxidant properties for the extract were obtained from flaxseeds using dichloromethane solvent. After the synthesis of gold and silver nanoparticles, the colloidal solutions of the prepared nanoparticles became purple and yellow, respectively. The absorption spectra of the gold and silver nanoparticles showed maximum wavelengths of 525 and 420 nm, respectively, which indicated the characteristic wavelengths for these nanoparticles. Transmission electron microscopy studies showed that the synthesized gold and silver nanoparticles had a spherical shape with uniform distribution and mean diameters of 40 and 90 nm, respectively.
 
Discussion & Conclusions: The results revealed that the use of different solvents was effective for the extraction of total phenolic content. There was also a direct relationship between the amount of total phenol and the results of measuring the antioxidant capacity of the extracts. Moreover, gold and silver nanoparticles were easily prepared using flaxseeds extract.
Keywords: Extract, Flaxseed, Gold nanoparticles, Linum usitatissimum L, Nanotechnology, Natural antioxidant, Silver nanoparticles
Full-Text [PDF 784 kb]   (646 Downloads)    
Type of Study: Research | Subject: Food chemistry
Received: 2020/07/20 | Accepted: 2020/12/23 | Published: 2021/03/30
References
1. Schewe LC, Sawhney VK, Davis AR. Ontogeny of floral organs in flax Linum usitatissimum Linaceae. ‎Am J Bot 2011; 98: 1077-85. doi.10.3732/ajb.1000431.
2. Allaby RG, Peterson GW, Merriwether DA, Fu YB. Evidence of the domestication history of flax Linum usitatissimum L. from genetic diversity of the sad2 locus. Theor Appl Gen2005; 112: 58-65. doi.10.1007/s00122-005-0112-2.
3. Shim YY, Gui B, Arnison PG, Wang Y, Reaney MJT. Flaxseed Linum usitatissimum L. bioactive compounds and peptide nomenclature a review. Trends Food Sci Tech 2014; 38: 5-20. doi.10.1016/j.tifs.2014.03.011.
4. Cunnane SC, Ganguli S, Menard C, Liede AC, Hamadeh M J, Chen ZY, et al. High α-linolenic acid flaxseed Linum usitatissimum some nutritional properties in humans. Br J Nutr 2007; 69: 443-53. doi.10.1079/bjn19930046.
5. Toure A, Xu X. Flaxseed Lignans source biosynthesis metabolism, antioxidant activity bio-active components and health benefits. Comp Rev Food Sci F 2010; 9: 261-9. doi.10.1111/j.1541-4337.2009.00105.x.
6. Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M. Synthesis of gold nanotriangles and silver nanoparticles using aloevera plant extract. Biotechnol Prog 2006; 22: 577-83. doi.10.1021/bp0501423.
7. Kumar V, Yadav SK. Plant mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 2009; 84: 151-7.doi.10.1002/jctb.2023.
8. Brewer MS. Natural antioxidants sources, compounds mechanisms of action and potential applications. Comp Rev 2011; 10: 221-7. doi.10.1111/j.1541-4337.2011.00156.x.
9. Gajjar P, Pettee B, Britt DW, Huang W, Johnson WP, Anderson A J. Antimicrobial activities of commercial nanoparticles against an environmental soil microbe pseudomonas putida KT 2440. J Biol Eng 2009; 3: 1-13. doi.10.1186/1754-1611-3-9.
10. Abdelhameed E-SS, Bazaid SA, Salman MS. Characterization of the phytochemical constituents of taif rose and its antioxidant and anticancer activities. Biomed Res Int 2013; 1-13. doi.10.1155/2013/345465.
11. Anwar F, Przybylski R. Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed Linum usitatissimum. Acta Sci Pol Technol Ali 2012; 11: 293-01.
12. Yaqoob N, Bhatti I, Bhatti H, Jamil A. Antioxidant potential of extracts from selected flaxseed cultivars Linum usitatissimum. Res J Appl Sci Eng Technol 2015; 10: 968-79. doi.10.19026/rjaset.10.1863.
13. Smitha SL, Philip D, Gopchandran KG. Green synthesis of gold nanoparticles using cinnamomum zeylanicum leaf broth. Spec Acta Mol Biomol Spec 2009; 74: 735-9. doi.10.1016/j.saa.2009.08.007.
14. Aragao AP, Oliveira TM, Quelemes PV, Perfeito MLG, Araujo MC, Santiago JDAS, et al. Green synthesis of silver nanoparticles using the seaweed gracilaria birdiae and their antibacterial activity. Arab J Chem 2019; 12: 4182-88. doi.10.1016/j.arabjc.2016.04.014.
15. Archana AS, Deepak MK. Synthesis of silver nanoparticles using flaxseed hydroalcoholic extract and its antimicrobial activity. Curr Biotechnol 2013; 2: 62-6. doi. 10.2174/22115501113029990007.
16. Bakar F, G. Caglayan M, Onur F, Nebioglu S, M. Palabiyik I. Gold nanoparticle-lignan complexes inhibited MCF7 cell proliferation in vitro a novel conjugation for cancer therapy. Ant Age Med Chem 2015; 15: 336-44. doi.10. 2174/1871520614666141202144152.
17. Kajla P, Sharma A, Sood DR. Flaxseed a potential functional food source. J Food Sci Tech 2015; 52: 1857-71. doi.10.1007/s13197-014-1293-y.
18. Rajesha J, Murthy KN, Kumar MK, Madhusudhan B, Ravishankar GA. Antioxidant potentials of flaxseed by in vivo model. J Agri Food Chem 2006 31; 54: 3794-99. doi.10.1021/jf053048a.
19. Ilokiassanga SB, Lewis LM, Espinoza CL, Salido AA, Angulo D, Pino J. Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of bucida buceras L. and phoradendron californicum. BMC Res Not2015; 8:1-14. doi.10.1186/s13104-015-1388-1.
20. Ngo TV, Scarlett CJ, Bowyer MC, Ngo PD, Vuong QV. Impact of different extraction solvents on bioactive compounds and antioxidant capacity from the root of salacia chinensis L. J Food Qual 2017; 2:123-8. doi.10.1155/2017/9305047.
21. Kaneda T, Yoshida H, Nakajima Y, Toishi M, Nugroho AE, Morita H. Cyclolinopeptides cyclic peptides from flaxseed with osteoclast differentiation inhibitory activity. Bioorg Med Chem Let 2016; 26: 1760-61. doi.10.1016/j.bmcl.2016.02.040.
22. Mohamedfeky F, Sherbiny I, Gmal Fedawy M, Abdelmogib M. Phytochemical studies on linum usitatissimum seeds and the nanoformulation of the bioactive butanol extract. In J Sci Eng Appl 2016; 5: 76-91. doi.10.7753/IJSE.1006.
23. Bekal M, Kumari S, Sharmila KP. Preliminary phytochemical screening of flax seed and assesment of its in vitro antioxidant activity. World J Pharm Pharm Sci 2015; 4: 952-58. doi.10.1186/1472-6882-10-21.
24. Zanwar AA, Hegde MV, Bodhankar SL. Cardioprotective activity of flax lignan concentrate extracted from seeds of Linum usitatissimum in isoprenalin induced myocardial necrosis in Rats. IntToxicol 2011; 4: 90-7. doi.10.2478/v10102-011-0016-8.
25. Wang H, Wang J, Qiu C, Ye Y, Guo X, Chen G, et al. Comparison of phytochemical profiles and health benefits in fiber and oil flaxseeds Linum usitatissimum. Food Chem 2017; 214: 227-33. doi.10.1016/j.foodchem.2016.07.075.
26. Shim YY, Gui B, Wang Y, JT. Reaney M. Flaxseed Linum usitatissimumL. oil processing and selected products a review. Tends Food Sci Tech J 2015; 43: 162-77. doi.
27. 1016/j.tifs.2015.03.001.
28. Qiu SX, Lu ZZ, Luyengi L, Lee SK, Pezzuto JM, Farnsworth NR, et al. Isolation and characterization of flaxseed Linum usitatissimum. Cons Pharm Biol 1999; 37: 1-7. doi.10.1076/phbi.37.1.1.632010.
29. Karamian R, Kamalnejad J. [Green synthesis of silver nanoparticles using aqueous seed extract of cuminum cyminum L. and evaluation of their biological activities]. J Ilam Uni Med Sci. 2019; 26: 128-41. (Persian) doi.10.29252/sjimu.26.5.128.
30. Mehrzadeh M, Valizadeh J, Ghasemi A. Characterization of effective parameters on the synthesized gold nanoparticles and investigating their antimicrobial activities using aqueous extract of hibiscus sabdariffa L. J Med Plant 2018; 16:107-22.
31. Azizianshermeh O, Valizadeh J, Noroozifar M, Qasemi A. [Investigating the antimicrobial activities of silver nanoparticles biosynthesized by aqueous extract of sambucus ebulus L.]. J Ilam Uni Med Sci 2016; 24: 92-107. (Persian) doi.10.18869/acadpub.sjimu.24.5.92.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: 123



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirzajani R, Kolahi M, Gorgian F. Phytochemical Study and Comparison of Antioxidant Potential and Phenolic Content of Different Solvent Extract of Flaxseed (Linum usitatissimum L.) and Biosynthesis of Gold and Silver Nanoparticles using its Extract. Journal of Ilam University of Medical Sciences 2021; 29 (1) :65-78
URL: http://sjimu.medilam.ac.ir/article-1-6678-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 29, Issue 1 (3-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.14 seconds with 31 queries by YEKTAWEB 4541