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:: Volume 29, Issue 4 (10-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(4): 18-27 Back to browse issues page
Protective Effect of Royal Jelly on Sperm Parameters, In-vitro Fertilizing Potential, and Oxidative Stress Indicators in the Testis of Mice Exposed to Ethephon
Ramin Jahangirfard *
Dept of Anatomy and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , r.jahangirfard@urmia.ac.ir
Abstract:   (1180 Views)
Introduction: Ethephon is an organophosphorus that used to stimulate plant growth. Royal Jelly is a substance with antioxidant properties and is effective for strengthening the immune system. This study aimed to determine the effect of Royal Jelly on sperm parameters, in vitro fertilization, and oxidative stress indicators in mice.
Material & Methods: In this experimental study, 20 adult male mice (NMRI strain) were divided into four control and experimental groups. The experimental group received Ethephon at 480 mg/kg. Royal Jelly was then administered orally and daily at a concentration of 200 mg/kg alone and in combination with Ethephon. After treatment, sperm samples were collected from the tail of epididymis to investigate sperm parameters and in vitro fertilizing potential; moreover, the testis tissue was taken for oxidative stress examination. A p-value less than 0.05 was considered statistically significant.
(Ethic code: IR-UU_AEC-811/پد/3)
Findings: A decrease in the sperm motility and viability, fertilization rate, blastocyst, and hatching embryos, as well as an increase in DNA damage, lack of nuclear maturation, and blocked embryos was observed in the experimental groups, compared to the control group. Moreover, Total Antioxidant Capacity and Malondialdehyde in the groups receiving Ethephon decreased and increased, respectively. However, Royal Jelly can prevent the adverse effects of oxidative stress (P<0.05).
Discussion & Conclusion: According to the results of this study, royal jelly can have an amelioration effect on epididymal sperm and in vitro fertilizing potential.
Keywords: Ethephon, In vitro fertilization, Oxidative stress, Royal jelly, Sperm parameters
Full-Text [PDF 588 kb]   (436 Downloads)    
Type of Study: Research | Subject: toxicology
Received: 2020/06/27 | Accepted: 2020/11/8 | Published: 2021/11/1
1. Yavuzkocaman A, Kılıç E. Evaluation of the genotoxicity of commercial formulations of ethephon and ethephon cyclanilide on Allium cepa L. root meristematic cells. Caryologia 2017; 70: 229-37-doi.10.1080/00087114.2017.1329960.
2. Rodwell DE, Spencer AG, Allen S. Teratology study in rats. Prep Int Res Dev Corp1980;3: 264-7. doi.10.1016/0041-008X (74)90107-0.
3. Ferrell J. 104-week chronic administration of ethrel in male and female rats. Final Pathol Rep Proj2013;747: 141-263. doi.10.4103/2319-4170.155589.
4. Vanmiller J. Lifetime dietary combined chronic toxicity and oncogenicity study with ethephon in albino Rats. Bushy Run Res Cen Rhone Poulenc 1989; 2:24-7. doi.10.4103/2319-4170.155589.
5. Klonne D. Supplemental historical control data requested for the lifetime dietary oncogenicity study with ethephon in albino Mice. Bushy Run Res Cent Rhone Poulenc ;1994;3: 17.
6. Victor VM, Rocha M, Iafuente M. Immune cell free radicals and antioxidants in sepsis. Int Immuno Pharmacol 2004; 4:327-47. doi.10.1016/j.intimp.2004.01.020.
7. Gholipour Z, Nejati V, Najafi G, Karimpour Z, Khaneshi F.] The protective effect of royal jelly on liver tissue of adult female rats with experimental polycystic ovarian syndrome [. Qom Uni Med Sci J 2014; 8:35-41. (Persian)
8. Lee JY, Baw CK, Gupta S, Aziz N, Agarwal A. Role of oxidative stress in polycystic ovary syndrome. Curr Wome Health Rev 2010:96-107. doi.10.2174/157340410791321336.
9. Dutta U. Evaluation of ethephon induced oxidative stress to gonadal disorder and its amelioration by ethanolic extract of shoot of Bambusa balcooa roxb. in albino Rat. Toxicol Lett 2015; 4:63. doi.10.1016%2Fj.toxlet.2015.08.776.
10. Sharma RK, Agarwal A. Role of reactive oxygen species in male infertility. Urology1996; 48: 835-50. doi.10.1016/S0090-4295(96)00313-5.
11. Narita Y, Ohta S, Suzuki KM, Nemoto T, Abe K, Mishima S. Effects of long term administration of royal jelly on pituitary weight and gene expression in middle aged female rats. Biosci Biotech Biochem 2009; 73:431-3. doi.10.1271/bbb.80556.
12. Abdelwahhab MA, Hassan AM, Amer HA, Naguib KM. Prevention of fumonisin induced maternal and developmental toxicity in Rats by certain plant extracts. J Appl Toxicol 2004; 24:469-74. doi.10.1002/jat.1000.
13. Wei W, Wei M, Kang XJ, Deng HH, Lu ZH. A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction. Electrophoresis 2009; 30:1949-52. doi.10.1002/elps.200800721.
14. Silici S, Ekmekcioglu O, Eraslan G, Demirtas A. Antioxidative effect of royal jelly in Cisplatin-induced testes damage. Urology 2009; 74:545-51. doi.10.1016/j.urology.2009.05.024.
15. Anand P, Thomas SG, Kunnumakkara AB, Sundaram C, Harikumar KB, Sung B. Biological activities of curcumin and its analogues made by man and mother. Biochem Pharmacol 2008; 76: 1590611. doi.10.1016/j.bcp.2008.08.008.
16. Jahangirfard R, Najafi G, Shalizar Jalali A, Ahmadi A. [The effect of ethephon investigation on sperm parameters and in vitro fertilizing potential in adult male Mice [. J Fasa Uni Med Sci 2020; 10: 2545-57. (Persian)
17. Malekian R, Abdanipour A, Sohrabi D. Antioxidant and neuroprotective effects of lycopene and insulin in the hippocampus of streptozotocin induced diabetic Rats. Biomed Rep 2019; 10: 47-54. [DOI:10.3892/br.2018.1171.]
18. Zambrano E, Rodriguez-Gonzalez GL, Guzman C, Garcia-Becerra R, Boeck L, Menjivar M, Larrea F, Nathanielsz PW. A maternal low Protein diet during Pregnancy and lactation in the Rat impairs male reproductive development. J Physiol 2005; 563: 275-84. doi.10.1113/jphysiol.2004.078543.
19. Mohamadghasemi F, Faghani M, Fallahkarkan M. The protective effect of melatonin on sperm parameters, Epididymis and Seminal vesicle morphology in Adult mouse treated with Busulfan. J Iran Anat Sci 2010; 10: 25-36.
20. Menkveld R, Wong WY, Lombard CJ, Wetzels AM, Thomas CM, Merkus HM, et al. Semen parameters including WHO and strict criteria morphology in a fertile and subfertile population: an effort towards standardization of invivo thresholds. Hum Rep 2001; 16: 1165-71. doi.10.1093/humrep/16.6.1165.
21. Nasr-Esfahani MH, Razavi S, Mardani M. Relation between different human sperm nuclear maturity tests and in vitro fertilization. J Assist Rep Gen2001; 18: 221-7.
22. Agarwal A, Saleh RA. Role of oxidants in male infertility rationale significance and treatment. Urol Clin North Am2002; 29: 817-27. doi.10.1016/S0094-0143(02)00081-2.
23. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fert Ster 2003; 79: 829-43. doi.10.1016/S0015-0282(02)04948-8.
24. Agarwal A, Sekhon LH. The role of antioxidant therapy in the treatment of male infertility. Hum Fert2010; 13: 217-25. doi.10.3109/14647273.2010.532279.
25. Varnet P, Fulton N, Wallace C, Aitken RJ. Analysis of a plasma membrane redox system in rat epididymal spermatozoa. Biol Reprod. 2001; 65: 13-1102. doi.10.1095/biolreprod65.4.1102.
26. Venkatesh S, Gurdeep Singh M, Prasad Gupta N, Kumar R, Deecaraman M, Dada R. Correlation of sperm morphology and oxidative stress in infertile men. Int J Rep Biomed 2009; 7: 29-34.
27. Abdeldaim MA, Tousson E, Elsayed IET, Awd WM. Ameliorative effects of saussurea lappa root aqueous extract against ethephon induced reproductive toxicity in male Rats. Environ Toxicol 2018;3: 1-10. doi.10.1002/tox.22669.
28. Attia SM. Dominant lethal mutations of topoisomerase II inhibitors etoposide and merbarone in male Mice a mechanistic study. Arch Toxicol2012; 86: 725-31.
29. Ghanbari E, Nejati V, Khazaei M. Antioxidant and protective effects of Royal jelly on histopathological changes in testis of diabetic rats. Int J Rep Biomed 2016; 14:519.
30. Jalali AS, Najafi G, Hosseinchi M, Sedighnia A. Royal Jelly alleviates sperm toxicity and improves in vitro fertilization outcome in stanozolol treated mice. Iranian J Rep Med2015; 13:15.
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Jahangirfard R. Protective Effect of Royal Jelly on Sperm Parameters, In-vitro Fertilizing Potential, and Oxidative Stress Indicators in the Testis of Mice Exposed to Ethephon. J. Ilam Uni. Med. Sci. 2021; 29 (4) :18-27
URL: http://sjimu.medilam.ac.ir/article-1-6635-en.html

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Volume 29, Issue 4 (10-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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