[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 28, Issue 4 (10-2020) ::
Journal of Ilam University of Medical Sciences 2020, 28(4): 100-112 Back to browse issues page
Stereological Study of the Protective Effect of Alpha-Lipoic Acid on Kidney Tissue following Toxicity with Silver Nanoparticles in NMRI Mice
Seyed Mohammad Ali Shariatzadeh * 1, Morteza Godarzi2
1- Dept of Biology, Faculty of Science, Arak University, Arak, Iran , s-shariatzadeh@araku.ac.ir
2- Dept of Biology, Faculty of Science, Arak University, Arak, Iran
Abstract:   (1484 Views)
Introduction: Today, with the advancements in technology and the use of silver nanoparticles (AgNPs)in various products, serious concerns have been raised about the use of this substance. This study aimed to determine the effect of Alpha Lipoic Acid(ALA) as a potent antioxidant against the toxicity of AgNPs on kidney tissue of NMRI mice.
 
Materials & Methods: In this experimental study, 24 adult male NMRI mice with a mean weight of 36±2 g were randomly allocated into four groups of control, AgNPs (500 mg/kg/day), ALA (100 mg/kg/day), and AgNPs+ALA. Subsequently, they were treated orally for 35 days and sacrificed. The left kidney was taken out, fixed, sectioned, processed, and stained using the hematoxylin-eosin method. Following that, the biochemical and stereological parameters of the kidney, such as the volume calculation of its various components were measured in this study. The data were analyzed in SPSS software (version 16). Ethics code: P/2/97s3113
 
 
Findings: In this study, there was a significant increase in the mean volume of the renal corpuscle (P<0.005), glomerulus (P<0.001), Taft (P<0.001), and Bowman capsule membrane (P<0.001); however, a significant decrease was observed in the mean total volume of Bowman capsule space (P<0.001) and proximal tubule lumen volume (P<0.05) in the AgNPs group, compared to the control group. The level of urea and malondialdehyde (P<0.001) was increased in the AgNPs group, compared to the control group.  In addition, total antioxidant capacity (P<0.001) showed a significant decrease.
 
Discussions & Conclusions: The ALA has a protective role in ameliorating kidney damage caused by silver nanoparticles.
Keywords: Silver Nanoparticles - Alpha Lipoic Acid - Kidney - Stereology - NMRI Mice
Full-Text [PDF 968 kb]   (624 Downloads)    
Type of Study: Research | Subject: biology
Received: 2019/12/18 | Accepted: 2020/06/22 | Published: 2020/10/31
References
1. Mody VV, Siwale R, Singh A, Mody HR. Introduction to metallic nanoparticles. J Pharm Bioal Sci 2010 2:282. doi.10.4103/ 0975-7406.72127
2. Khan I, Saeed K, Khan I. Nanoparticles properties applications and toxicities. Arabian J Chem2019; 12:908-31. doi.10. 1016/j.arabjc.2017.05.011
3. Hofmann M, Grainger DW, Hofmann H. Nanoparticles in medicine current chall-enges facing inorganic nanoparticle toxicity assessments and standardizations. Nanomed Nanotechnol Biol Med2015; 11:1689-94. doi: 10.1016/j.nano.2015.05.005
4. Koller M, Saleh HM. Introductory chapter introducing heavy metals. 2 th ed. Intech Open Publication.2018; P.201-7. doi: 10.5772/intechopen.74783
5. Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver nanoparticles synthesis characterization properties applications and therapeutic approaches. Int J Mole Sci 2016; 17:1534. doi.10.3390/ijms17091534
6. Habibian S, Shadnoush SH, Arabi M, Saffar B. [Evaluation of cell toxicity and protein expression induced by silver nanoparticles in sperm and testicles of Rats]. J Shahrekord Uni Med Sci2013; 15: 26-34. (Persian)
7. Matteis V. Exposure to inorganic nanoparticles routes of entry immune response biodistribution and in vitro in vivo toxicity evaluation. Toxics2017; 5:29. doi.10.3390/toxics5040029
8. Volker C, Oetken M, Oehlmann J. The biological effects and possible modes of action of nanosilver. Rev EnvironCont Toxicol 2013; 223: 81-106. doi.10.1007/978-1-4614-5577-6_4
9. Dakhil A. Biosynthesis of silver nanoparticle AgNPs using Lactobacillus and their effects on oxidative stress biomarkers in Rats. J King Saud Uni Sci2017; 29:462-7. doi.10.1016/j.jksus.2017.05.013
10. Gorąca A, Hukkolega H, Piechota A, Kleniewska P, Ciejka E, Skibska B. Lipoic acid biological activity and therapeutic potential. Pharmacol Rep 2011; 63:849-58. doi.10.1016/s1734-1140(11)70600-4
11. Packer L, Witt EH, Tritschler HJ. Alpha lipoic acid as a biological antioxidant. Free Rad Biol Med1995; 19:227-50. doi.10.1016/ 0891-5849(95)00017-r
12. Shay KP, Moreau RF, Smith EJ, Smith AR, Hagen TM. Alpha lipoic acid as a dietary supplement molecular mechanisms and therapeutic potential. Biochim Biophys Acta Gene Sub2009; 1790:1149-60. doi.10.1016/j.bbagen.2009.07.026
13. Almansour M, Jarrar Q, Battah A, Jarrar B. Morphometric alterations induced by the toxicity of variable sizes of silver nanoparticles. Int J Morphol2015; 33: 544-552. doi.10.4067/S0717-9502201500 0200022.
14. Kim YS, Song MY, Park JD, Song KS, Ryu HR, Chung YH, et al. Subchronic oral toxicity of silver nanoparticles. Part Fibre Toxicol2010; 7:1-11. doi. 10.1186/1743-8977-7-20
15. Armagan I, Bayram D, Candan IA, Yigit A, Celik E, Armagan HH, et al. Effects of pentoxifylline and alpha lipoic acid on methotrexate-induced damage in liver and kidney of Rats. Environ Toxicol Pharmacol 2015; 39:1122-31. doi. 10.1016/j.etap. 2015.04.003
16. Carlos A. Mandarim de L. Stereological tools in biomedical research. Anais Acad Brasileira Cien2003;75: 469-86. doi.10.1590/s0001-37652003000400006
17. Murmu S, Shrivastava VK. Protective Action of an anti-oxidant vitamin C against bisphenol toxicity in Cirrhinus mrigala. Turkish J Fish Aquat Sci 2011;11: 25-9. doi.10.4194/trjfas.2011.0104
18. Howard V, Reed M. Unbiased stereology: three dimensional measurements in microscopy. Gar Sci2004. doi.10.1046/j. 1469-7580.1999. 194101536.x
19. Hoseini L, Roozbeh J, Sagheb M, Karbalaydoust S, Noorafshan A. [Nandrolone decanoate increases the volume but not the length of the proximal and distal convoluted tubules of the Mouse kidney]. Micron2009; 40: 226-30. doi.10.1016/j.micron.2008.08.004. (Persian)
20. Buege JA, Aust S D. Microsomal lipid peroxidation. Meth Enzymol Acad1978; 52: 302-10. doi. 10.1016/s0076-6879(78)52032-6
21. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Meth Enzymol Acad 1990; 186: 407-21. doi.10.1016/0076-6879(90)86134-h
22. Benzie IF, Strain JJ. The ferric reducing ability of plasma as a measure of antioxidant power the FRAP assay. Analyt Biochem 1996; 239: 70-6. doi.10.1006/abio.1996.0292
23. Asare N, Instanes C, Sandberg WJ, Refsnes M, Schwarze P, Kruszewski M, et al. Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells. Toxicology2012; 291:65-72. doi. 10.1016/j.tox.2011.10.022
24. Prabhu S, Poulose EK. Silver nanoparticles mechanism of antimicrobial action synthesis medical applications and toxicity effects. Int Nano let 2012; 2:32. doi.10.1186/2228-5326-2-32
25. Kimura H, Sawada T, Oshima S, Kozawa K, Ishioka T, Kato M. Toxicity and roles of reactive oxygen species. Curr Drug Targ Inflam Allerg2005; 4: 489-95.doi.10.2174/1568010054526287
26. Akilesh S. Normal kidney function and structure. 1 th ed. Uni Washington Seat USA .2014; P.2716-33. doi.10.1016/B978-0-12-386456-7.05402-2
27. Heidari Z, Mahmoudzadehsagheb HR, Dezfoulian AR, Barbarestani M, Noori SMH. [A stereological analysis of renal glomeruli following chronic lead intoxication in Rat during a continuous period of 8 weeks]. Acta Med Iranica 2002; 40: 73-8. (Persian)
28. Forbes JM, Cooper ME, Oldfield MD, Thomas MC. Role of advanced glycation end products in diabetic nephropathy. J Am Soc Nephrol.2003; 14: 254-8. doi.10.1097/01.asn.0000077413.41276.17
29. Yamagishi SI, Imaizumi T. Diabetic vascular complications: pathophysiology, biochemical basis and potential therapeutic strategy. Curr Pharmaceut Des 2005; 11: 2279-99. doi. 10.2174/1381612054367300
30. Valencia A, Morán J. Reactive oxygen species induce different cell death mechanisms in cultured neurons. Free Rad Biol Med 2004; 36:1112-25. doi.10.1016/j.freeradbiomed.2004.02.013
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: 3113



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shariatzadeh S M A, Godarzi M. Stereological Study of the Protective Effect of Alpha-Lipoic Acid on Kidney Tissue following Toxicity with Silver Nanoparticles in NMRI Mice. sjimu 2020; 28 (4) :100-112
URL: http://sjimu.medilam.ac.ir/article-1-6229-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 4 (10-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.14 seconds with 31 queries by YEKTAWEB 4536
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)