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Comparison of Serum Level and IL-18 Gene Expression and Reactive Protein in Patients with Type 2 Diabetes with Metabolic
Syndrome and Healthy People
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Leila Yadolahi Farsani1    , Noosha Zia-jahromi * 2, Mohammad Vaezipour3 |
1- Dept of Basic Science, Faculty of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Dept of Basic Science, Faculty of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , nooshazia.59@gmail.com
3- Faculty of Medical Sciences, Shahid Beheshti University, Tehran, Iran |
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Abstract: (3440 Views) |
Introduction: Metabolic syndrome is a common clinical anomaly, with an increased risk of developing type 2 diabetes and cardiovascular risk factors. Increased inflammatory mediators, such as reactive protein,as well as the inflammatory cytokines can be associated with the incidence and progression of metaboli syndrome and type 2 diabetes.
Materials & Methods: This study was performed on 35 male and female patients with an average age of 55 years, type 2 diabetes mellitus with metabolic syndrome and 35 healthy subjects as control group. Separated serum samples were kept at -20 ° C for quantitative evaluation of serum IL-18 by ELISA kit and C-reactive protein. RNA extraction from cells was performed by JenaBioscience kit and finally Gene expression assay was performed quantitatively using Real Time RTPCR.
Findings: The mean serum levels of IL-18 were significantly increased (P=0.008), and serum reactive protein (P<0.001) was significantly increased in patients with type 2 diabetes mellitus with metabolic syndrome compared with healthy subjects. Also, expression of IL-18 gene (P=0.003) in lymphocyte cells of type II diabetes patients with metabolic syndrome was significantly higher than healthy subjects.
Discussion & Conclusions: The present study showed that patients with type 2 diabetes mellitus with metabolic syndrome have high levels of inflammatory factors such as IL-18 in their serum. it can be concluded that serum levels of IL-18, C-reactive protein and IL-18 expression play an important role in inflammatory response and progression. Type 2 diabetes and metabolic syndrome. |
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| Keywords: Interleukin 18, C-reactive protein, Type II diabetes, Metabolic syndrome |
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Full-Text [PDF 722 kb]
(1713 Downloads)
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Type of Study: Research |
Subject:
biology
Received: 2018/08/6 | Accepted: 2019/01/20 | Published: 2019/09/15
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