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:: Volume 25, Issue 6 (3-2018) ::
Journal of Ilam University of Medical Sciences 2018, 25(6): 141-149 Back to browse issues page
An evaluation of Neospora caninum Tachyzoites Cultivation in Attachment (Vero) and Suspension (Mouse macrophage) Cell Lines
Moniereh Khordadmehr *
Dept of Pathobiology, Faculty of Veterinary, Tabriz University, Tabriz, Iran , khordadmehr@tabrizu.ac.ir
Abstract:   (4685 Views)
Introduction: Neospora caninum is an intracellular protozoan considered as a serious disease agent in cattle and dogs. In the present study, N. caninum tachyzoites were cultivated in mouse macrophage cell line and Vero cell line.
 
Materials & Methods: The number of harvested tachyzoites was recorded daily. After parasite propagation and continuous passages, chicken embryonated eggs were used for comparison of tachyzoites attenuation. Mortality rate was recorded daily and the tissue samples of brain, liver, and heart were taken for histopathological examination.
 
Findings: Mortality rate in embryonated eggs inoculated with N. caninum harvested from Vero and mouse macrophage cell line was 100% and 10%, respectively, which was dose-dependent. Microscopically, there were milder hepatitis and myocarditis in the liver and heart of chickens inoculated with N. caninum harvested from mouse macrophage cell line.
 
Discussion & Conclusions: The present findings showed that cultivation of N. caninum tachyzoites in macrophage cell line increased propagation of tachyzoites and also reduced attenuation of the parasite. These results suggested the suitable cell line for mass production of N. caninum tachyzoites for various experimental researches and also producing live attenuated vaccine.
Keywords: Cell culture, Attenuation, Embryonated egg, Histopathology
Full-Text [PDF 680 kb]   (1677 Downloads)    
Type of Study: Research |
Received: 2017/09/12 | Accepted: 2018/01/8 | Published: 2018/03/14
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Khordadmehr M. An evaluation of Neospora caninum Tachyzoites Cultivation in Attachment (Vero) and Suspension (Mouse macrophage) Cell Lines . J. Ilam Uni. Med. Sci. 2018; 25 (6) :141-149
URL: http://sjimu.medilam.ac.ir/article-1-4565-en.html


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Volume 25, Issue 6 (3-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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