[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 26, Issue 5 (1-2019) ::
Journal of Ilam University of Medical Sciences 2019, 26(5): 128-141 Back to browse issues page
Green synthesis of silver nanoparticles using aqueous seed extract of Cuminum cyminum L. and evaluation of their biological activities
Roya Karamian * 1, Jamaledin Kamalnejade2
1- Dept of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran , R_karamian@basu.ac.ir
2- Dept of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran
Abstract:   (3895 Views)
Introduction: There are many reports on the application of medicinal plants in traditional medicine, as well as numerous applications of metallic nanoparticles in different biomedical fields. Plant extract mediated green synthesis of nanoparticles and investigations on their therapeutic effects are new concepts. The medicinal plant of Cuminum cyminum L. includes a wide range of secondary metabolites with a high reducing power, which can be used for green biosynthesis of silver nanoparticles. The main aims of this study were phytochemical analysis of seed aqueous extract of Cuminum cyminum, biosynthesis of silver nanoparticles using this extract, the achievement of optimization condition for the synthesis of silver nanoparticles, and assessment of biological activaties of both the extract and the synthesized nanoparticles.
Materials & Methods: Total phenol and flavonoid, reducing sugar, starch, and ascorbic acid contents were measured by Folin-Ciocalteo, aluminum chloride, dinitrosalicylic acid, anthrone, and dinitrophenyl hydrazine reagents, respectively. The Ag+ ions reduction and characterization of silver nanoparticles were assessed by UV-Visible spectrophotometry, X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy. Antioxidant activity of the samples was screened by DPPH free radical scavenging. Antibacterial activity of the samples was also evaluated against four gram-positive and -negative bacteria namely, Bacillus cereus (PTCC 1247), Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 35218), and Pseudomonas aeruginosa (ATCC 27853) by disc diffusion method. In addition, antifungal activity was assessed against Fusarium oxysporum.
Findings: The indings indicated that the seed extract contained the high amounts of total phenolic and flavonoidic compositions, as well as sugar, starch, and ascorbic acid. Different analyses showed that the mean size of the synthesized nanoparticles was 5-45 nm in the optimal condition. The samples had proper antioxidant potential (IC50=1.35-1.67 mg/ml) and the extract in combination with silver nanoparticles represented synergistic effect in DPPH free radical scavenging (IC50=1.35 mg/ml). In addition, the investiogated samples had a good antibacterial activity against some of tested bacteria and also antifungal activity against Fusarium oxysporum.
Discussion & Conclusions: It seems that the secondary metabolites of Cuminum cyminum have good potential for the reduction and stabilization of the synthesized Ag nanoparticles. Both the seed extract and synthesized nanoparticles using this extract have considerable biological activities and may be used in antioxidant nutrient production or medicinal supplements in future.
Keywords: Antibacterial activity, Antifungal activity, Antioxidant activity, Cuminum cyminum L., Silver nanoparticles
Full-Text [PDF 882 kb]   (3809 Downloads)    
Type of Study: Research | Subject: biology
Received: 2017/01/14 | Accepted: 2017/06/20 | Published: 2019/01/15
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karamian R, Kamalnejade J. Green synthesis of silver nanoparticles using aqueous seed extract of Cuminum cyminum L. and evaluation of their biological activities. Journal of Ilam University of Medical Sciences 2019; 26 (5) :128-141
URL: http://sjimu.medilam.ac.ir/article-1-4076-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 26, Issue 5 (1-2019) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.13 seconds with 31 queries by YEKTAWEB 4541