[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations62733583
h-index2719
i10-index18478

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 25, Issue 1 (5-2017) ::
Journal of Ilam University of Medical Sciences 2017, 25(1): 180-194 Back to browse issues page
Evaluation of Chemical Composition of Helichrysum artemisioides Extract Its Effect on Biofilm Formation and IcaD Gene Expression in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus
Amir Mirzaie * 1, Hassan Noorbazargan2 , Seyed Hamid Reza Khatami Khatami3 , Seyed Ataollah Sadat Shandiz4 , Arian Rahimi5 , Ali asghar Bagheri keshtali6
1- Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran , Amir_mirzaie92@yahoo.com
2- Dept of Biotechnology, Faculty of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Dept of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran, Iran
4- Dept of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
5- Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, Tehran, Iran
6- Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Abstract:   (6694 Views)

Introduction: Staphylococcus aureus is a major causative agent of nosocomial infections which produces a wide range of diseases due to biofilm formation. This study aimed to investigate the phytochemical composition of Helichrysum artemisioides extract, its anti-biofilm effects on clinical isolates of ­methicillin-resistant Staphylococcus aureus, as well as, analysis of icaD gene expression.

Materials & methods: First, the phytochemical composition of H. artemisioides extract was studied by GC/MS method. Then, the biofilm formation capacity of 50 clinical isolates of S. aureus was evaluated by Congo red agar and polymerase chain reaction (PCR). Finally, after treatment of strains with SubMIC ­concentration of the extract within 24 hours, the expression level of icaD gene was evaluated by Real Time PCR.

Findings: The phytochemical analysis of extract showed 52 compounds, including α-Pinene (12.5%) and Carvacrol (8.6%) as the major components. The evaluation of biofilm formation test shows that out of 50 strains of S. aureus, 10 strains (20%) were positive for biofilms formation. Also, after treatment of selected strains by SubMIC concentration of the extract, the gene expression quantitation of icaD was down-regulated significantly (P<0.05).

Discusion & conclusions: Due to the significant effects of H. artemisioides extract on biofilm formation, it seems that the extract could be used as complementary therapy to inhibit S. aureus biofilm.

Keywords: Helichrysum artemisiodes, Staphylococcus areus, Biofilm, icaD
Full-Text [PDF 771 kb]   (4672 Downloads)    
Type of Study: Research |
Received: 2016/10/8 | Accepted: 2017/01/14 | Published: 2017/05/17
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirzaie A, Noorbazargan H, Khatami S H R K, Sadat Shandiz S A, Rahimi A, Bagheri keshtali A A. Evaluation of Chemical Composition of Helichrysum artemisioides Extract Its Effect on Biofilm Formation and IcaD Gene Expression in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus. J. Ilam Uni. Med. Sci. 2017; 25 (1) :180-194
URL: http://sjimu.medilam.ac.ir/article-1-3863-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 25, Issue 1 (5-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.15 seconds with 40 queries by YEKTAWEB 4643