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:: Volume 29, Issue 1 (3-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(1): 79-86 Back to browse issues page
Association of Rs13476 Polymorphism in lncRNA GAS5 Encoding Gene with Type 2 Diabetes in Isfahan, Iran
Asefeh Amini1 , Maryam Peymani * 2
1- Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , peymani62_m@yahoo.com
Abstract:   (1933 Views)
Introduction: Gas5 lncRNA is a long non-coded RNA that is involved in regulating gene expression. Studies have shown that the expression of GAS5 lncRNA is reduced in the serum of diabetic patients. Considering the role of GAS5 lncRNA, the present study aimed to investigate the association between rs13476 polymorphism in the gene encoding this lncRNA with the incidence of type 2 diabetes in Isfahan, Iran.
 
Materials & Methods: This case-control study was conducted on 200 healthy individuals and 200 patients with type 2 diabetes who were randomly selected from the target population. The individuals' genotypes were determined for the polymorphism using the PCR-RFLP method, and some samples were sequenced to confirm the results. Afterward, the frequency of genotypes, and consequently, the frequency of alleles was analyzed in order to obtain the relationship of this polymorphism with risk of type 2 diabetes, gender, and disease incidence.
Ethics code: IR.IAU.SHK.REC.1398.021
 
Findings: After amplification of 380 bp fragment containing rs13476 polymorphism in all samples and then enzymatic digestion by BCCI, all samples showed TT genotype. Moreover, the sequence results of a number of samples confirmed the results of enzymatic digestion. The frequency of TT genotype in the target population was 400 cases (100%), and the frequency of T allele was 800 cases. On the other hand, allele A was not observed in the target population.
 
Discussions & Conclusions: Due to the lack of association between polymorphism and risk of diabetes in this population, this polymorphism cannot be used to screen diabetic patients in Isfahan, Iran. Further studies are required to evaluate the importance of polymorphism studied in different populations.
Keywords: Diabetes type 2, Inc-GAS5, SNP Polymorphism
Full-Text [PDF 648 kb]   (563 Downloads)    
Type of Study: Research | Subject: Molecular Genetics
Received: 2020/03/2 | Accepted: 2020/12/27 | Published: 2021/03/30
References
1. Carter G, Miladinovic B, Patel AA, Deland L, Mastorides S, Patel NA. Circulating long noncoding RNA GAS5 levels are correlated to prevalence of type 2 diabetes mellitus. BBA clinical. 2015;4:102-7. doi.10.1016/j.bbacli.2015.09.001.
2. Sari S, Badr R. The association of miR-27a (rs895819) polymorphism with the risk of type 2 diabetes. J Shahrekord Uui Med Sci 2017;19:22-8.
3. Shi Y, Parag S, Patel R, Lui A, Murr M, Cai J, et al. Stabilization of lncRNA GAS5 by a small molecule and its implications in diabetic adipocytes. Cell Chem Biol 2019;26:319 330. doi.10.1016/j.chembiol.2018.11.012.
4. Jalali S, Bhartiya D, Lalwani MK, Sivasubbu S, Scaria V. Systematic transcriptome wide analysis of lncRNA-miRNA interactions. Plos One 2013;8:53823. doi. 10.1371/journal.pone.0053823.
5. Bayoumi AS, Sayed A, Broskova Z, Teoh JP, Wilson J, Su H, et al. Crosstalk between long noncoding RNAs and microRNAs in health and disease. Int J Mole Sci 2016;17:356. doi. 10.3390/ijms17030356.
6. Minotti L, Agnoletto C, Baldassari F, Corra F, Volinia S. SNPs and somatic mutation on long non coding RNA new frontier in the cancer studies? High Throughput 2018 ; 7:131-6. doi.10.3390/ht7040034.
7. Huang X, Zhang W, Shao Z. Association between long non coding RNA polymorphisms and cancer risk a meta analysis. Biosci Rep 2018;38:121-8. doi.10.1042/BSR20180365.
8. Castellanos A, Ghosh S. Disease associated SNPs in Inflammation related lncRNAs. Front Immunol2019;10:420. doi.10.3389/fimmu.2019.00420.
9. Gong J, Liu W, Zhang J, Miao X, Guo AY. lncRNASNP a database of SNPs in lncRNAs and their potential functions in human and mouse. Nucle Acids Res 2014;43:181-6. doi.10.1093/nar/gku1000.
10. Alberti KG, Zimmet PZ. Definition diagnosis and classification of diabetes mellitus and its complications. Diabet Med 1998;15:539-53. doi.10.1002/(SICI)1096-9136(199807)15:7<539
11. Cash JC, Glass CA. Family practice guidelines. 1th ed. Springer Publication Comp. 2010;P.2114.
12. Cheng J, Kapranov P, Drenkow J, Dike S, Brubaker S, Patel S, et al. Transcriptional maps of 10 human chromosomes at 5nucleotide resolution. Science 2005; 308:1149-54. doi. 10.1126/science.1108625
13. Kino T, Hurt DE, Ichijo T, Nader N, Chrousos GP. Noncoding RNA gas5 is a growth arrest and starvation associated repressor of the glucocorticoid receptor. Sci Sig2010;3:8. doi. 10.1126/scisignal.2000568.
14. Fawzy MS, Abdelghany AA, Toraih EA, Mohamed AM. Circulating long noncoding RNAs H19 and GAS5 are associated with type 2 diabetes but not with diabetic retinopathy a preliminary study. Bosn J Bas Med Sci2020;20:365-371. doi.10.17305/bjbms.2019.4533.
15. Ge X, Xu B, Xu W. Long noncoding RNA GAS5 inhibits cell proliferation and fibrosis in diabetic nephropathy by sponging miR221 and modulating SIRT1 expression. Ag Albany NY2019;11:8745-9. doi.10.18632/aging.102249.
16. Li Z, Yu Z, Meng X, Zhou S, Xiao S, Li X. Long noncoding RNA GAS5 impairs the proliferation and invasion of endometrial carcinoma induced by high glucose via targeting miR222-3pp27. Am J Trans Res 2019 ;11:2413-21.
17. Chen F, Li Y, Li M, Wang L. Long noncoding RNA GAS5 inhibits metastasis by targeting miR-182ANGPTL1 in hepatocellular carcinoma. Am J Cancer Res 2019;9:108-21.
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Ethics code: IR.IAU.SHK.REC.1398.021

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Amini A, Peymani M. Association of Rs13476 Polymorphism in lncRNA GAS5 Encoding Gene with Type 2 Diabetes in Isfahan, Iran. J. Ilam Uni. Med. Sci. 2021; 29 (1) :79-86
URL: http://sjimu.medilam.ac.ir/article-1-6389-en.html
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Volume 29, Issue 1 (3-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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