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:: Volume 29, Issue 1 (3-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(1): 79-86 Back to browse issues page
Association of Rs13476 Polymorphism in lncRNA GAS5 Encoding Gene with Type 2 Diabetes in Isfahan, Iran
Asefeh Amini1 , Maryam Peymani * 2
1- Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , peymani62_m@yahoo.com
Abstract:   (1062 Views)
Introduction: Gas5 lncRNA is a long non-coded RNA that is involved in regulating gene expression. Studies have shown that the expression of GAS5 lncRNA is reduced in the serum of diabetic patients. Considering the role of GAS5 lncRNA, the present study aimed to investigate the association between rs13476 polymorphism in the gene encoding this lncRNA with the incidence of type 2 diabetes in Isfahan, Iran.
Materials & Methods: This case-control study was conducted on 200 healthy individuals and 200 patients with type 2 diabetes who were randomly selected from the target population. The individuals' genotypes were determined for the polymorphism using the PCR-RFLP method, and some samples were sequenced to confirm the results. Afterward, the frequency of genotypes, and consequently, the frequency of alleles was analyzed in order to obtain the relationship of this polymorphism with risk of type 2 diabetes, gender, and disease incidence.
Ethics code: IR.IAU.SHK.REC.1398.021
Findings: After amplification of 380 bp fragment containing rs13476 polymorphism in all samples and then enzymatic digestion by BCCI, all samples showed TT genotype. Moreover, the sequence results of a number of samples confirmed the results of enzymatic digestion. The frequency of TT genotype in the target population was 400 cases (100%), and the frequency of T allele was 800 cases. On the other hand, allele A was not observed in the target population.
Discussions & Conclusions: Due to the lack of association between polymorphism and risk of diabetes in this population, this polymorphism cannot be used to screen diabetic patients in Isfahan, Iran. Further studies are required to evaluate the importance of polymorphism studied in different populations.
Keywords: Diabetes type 2, Inc-GAS5, SNP Polymorphism
Full-Text [PDF 648 kb]   (294 Downloads)    
Type of Study: Research | Subject: Molecular Genetics
Received: 2020/03/2 | Accepted: 2020/12/27 | Published: 2021/03/30
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Ethics code: IR.IAU.SHK.REC.1398.021

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Amini A, Peymani M. Association of Rs13476 Polymorphism in lncRNA GAS5 Encoding Gene with Type 2 Diabetes in Isfahan, Iran. Journal of Ilam University of Medical Sciences 2021; 29 (1) :79-86
URL: http://sjimu.medilam.ac.ir/article-1-6389-en.html

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