[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations62733583
h-index2719
i10-index18478

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 24, Issue 1 (4-2016) ::
Journal of Ilam University of Medical Sciences 2016, 24(1): 18-27 Back to browse issues page
Evaluation of Magnetic Silica Nanoparticles Treated with In-111 in SKBR-3 Cell Line
M Hasanvand1 , Fatemeh Keshavarzi * 2, P Ashtari3 , B Alirezapoor3
1- Islamic Azad University, Sanandaj
2- Islamic Azad University, Sanandaj , gol.keshavarzi@gmail.com
3- Nuclear Science and Technology Research Institute
Abstract:   (6582 Views)

Introduction: Magnetic nanoparticles are as nanoscale materials causing a great revolution in the diagnosis and treatment methods in medical science. Suitable half-life of the In-111 radioisotope makes use for in vivo studies. In this study, in order to obtain optimal absorption and stability the first synthesis of the silicate magnetic nanoparticles (Fe3O4@SiO2) and stabilization of the In-111 radioisotope were investigated. Then, the permeability was examined in SKBR3 breast cancer cell line for using the diagnosis of diseases.

Materials & methods: The magnetite core was prepared by precipitation method. Then it was used as the core for the synthesis of magnetic silicate nanoparticles. Nanoparticles were synthesized according to sol-gel method in the reverse micro emulsion using tetraetoxy silane (TEOS) and 3-amino propyl tree-ataxia silane (APTS) as the monomers and precursors. Then the In-111 radioisotope adsorbed on the surface of the magnetite silicate nanoparticles and formed the radio-conjugated. Finally, the entry of the radio-conjugated nanoparticles on SKBR-3, breast cancer cell line, is studied through the cell culture.

Findings: TEM results were shown the average size of the nanoparticles about 40 NM. The size is suitable for biological applications. The radio-analysis revealed more than 92 percent of the primary In-111stablized on the nanoparticles. The nanoparticles cell culture results are revealed the highest entrance efficiency about 26-27%- during the first hour from beginning the cultivation. The stability tests results revealed the stabilized radio-conjugated are stable during of washing and scattering and then considered as stable conjugation.

Discussion & Conclusions: Due to the unique properties of In-111the mentioned nanoparticles, which are prepared using nano biotechnological methods would be able to apply for diagnosis purposes.

Keywords: Magnetic nanoparticles, In-111, SKBR3 cell line, Breast cancer
Full-Text [PDF 651 kb]   (2803 Downloads)    
Type of Study: Research | Subject: biochemistry
Received: 2014/10/17 | Accepted: 2015/09/20 | Published: 2016/04/12
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hasanvand M, keshavarzi F, Ashtari P, Alirezapoor B. Evaluation of Magnetic Silica Nanoparticles Treated with In-111 in SKBR-3 Cell Line. J. Ilam Uni. Med. Sci. 2016; 24 (1) :18-27
URL: http://sjimu.medilam.ac.ir/article-1-2213-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 24, Issue 1 (4-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.16 seconds with 41 queries by YEKTAWEB 4643