Introduction: Magnetic nanoparticles are as nanoscale materials causing a great revolution in the diagnosis and treatment methods in medical science. Suitable half-life of the In-111 radioisotope makes use for in vivo studies. In this study, in order to obtain optimal absorption and stability the first synthesis of the silicate magnetic nanoparticles (Fe₃O₄@SiO₂) and stabilization of the In-111 radioisotope were investigated. Then, the permeability was examined in SKBR3 breast cancer cell line for using the diagnosis of diseases.

Materials & methods: The magnetite core was prepared by precipitation method. Then it was used as the core for the synthesis of magnetic silicate nanoparticles. Nanoparticles were synthesized according to sol-gel method in the reverse micro emulsion using tetraetoxy silane (TEOS) and 3-amino propyl tree-ataxia silane (APTS) as the monomers and precursors. Then the In-111 radioisotope adsorbed on the surface of the magnetite silicate nanoparticles and formed the radio-conjugated. Finally, the entry of the radio-conjugated nanoparticles on SKBR-3, breast cancer cell line, is studied through the cell culture.

Findings: TEM results were shown the average size of the nanoparticles about 40 NM. The size is suitable for biological applications. The radio-analysis revealed more than 92 percent of the primary In-111stablized on the nanoparticles. The nanoparticles cell culture results are revealed the highest entrance efficiency about 26-27%- during the first hour from beginning the cultivation. The stability tests results revealed the stabilized radio-conjugated are stable during of washing and scattering and then considered as stable conjugation.

Discussion & Conclusions: Due to the unique properties of In-111the mentioned nanoparticles, which are prepared using nano biotechnological methods would be able to apply for diagnosis purposes.