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Journal of Ilam University of Medical Sciences 2016, 24(1): 18-27 Back to browse issues page
Evaluation of Magnetic Silica Nanoparticles Treated with In-111 in SKBR-3 Cell Line
M Hasanvand1, Fatemeh Keshavarzi *2, P Ashtari3, B Alirezapoor3
1- Islamic Azad University, Sanandaj
2- Islamic Azad University, Sanandaj , gol.keshavarzi@gmail.com
3- Nuclear Science and Technology Research Institute
Abstract:   (6130 Views)

Introduction: Magnetic nanoparticles are as nanoscale materials causing a great revolution in the diagnosis and treatment methods in medical science. Suitable half-life of the In-111 radioisotope makes use for in vivo studies. In this study, in order to obtain optimal absorption and stability the first synthesis of the silicate magnetic nanoparticles (Fe3O4@SiO2) and stabilization of the In-111 radioisotope were investigated. Then, the permeability was examined in SKBR3 breast cancer cell line for using the diagnosis of diseases.

Materials & methods: The magnetite core was prepared by precipitation method. Then it was used as the core for the synthesis of magnetic silicate nanoparticles. Nanoparticles were synthesized according to sol-gel method in the reverse micro emulsion using tetraetoxy silane (TEOS) and 3-amino propyl tree-ataxia silane (APTS) as the monomers and precursors. Then the In-111 radioisotope adsorbed on the surface of the magnetite silicate nanoparticles and formed the radio-conjugated. Finally, the entry of the radio-conjugated nanoparticles on SKBR-3, breast cancer cell line, is studied through the cell culture.

Findings: TEM results were shown the average size of the nanoparticles about 40 NM. The size is suitable for biological applications. The radio-analysis revealed more than 92 percent of the primary In-111stablized on the nanoparticles. The nanoparticles cell culture results are revealed the highest entrance efficiency about 26-27%- during the first hour from beginning the cultivation. The stability tests results revealed the stabilized radio-conjugated are stable during of washing and scattering and then considered as stable conjugation.

Discussion & Conclusions: Due to the unique properties of In-111the mentioned nanoparticles, which are prepared using nano biotechnological methods would be able to apply for diagnosis purposes.

Keywords: Magnetic nanoparticles, In-111, SKBR3 cell line, Breast cancer
Full-Text [PDF 651 kb]   (2531 Downloads)    
Type of Study: Research | Subject: biochemistry
Received: 2014/10/17 | Accepted: 2015/09/20 | Published: 2016/04/12
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Hasanvand M, keshavarzi F, Ashtari P, Alirezapoor B. Evaluation of Magnetic Silica Nanoparticles Treated with In-111 in SKBR-3 Cell Line. Journal of Ilam University of Medical Sciences 2016; 24 (1) :18-27
URL: http://sjimu.medilam.ac.ir/article-1-2213-en.html


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Volume 24, Issue 1 (4-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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