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Isolation of Adherent CD 133 Positive from Cord Blood Unrestricted somatic stem cell its rare population and Nominate as a Valuable Source to Tissue Engineering
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S Haydari kashl   , M Rezaee tavirani * 1, M Ebrahimi , R Roozafzoon , F Frozanfar , H Jahani |
| 1- , rezaei.tavirani@ibb.ut.ac.ir |
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Abstract: (14861 Views) |
| Introduction: Stem cells have the ability repair damaged tissues are too. Mesenchymal stem cells from tissues such as bone marrow, peripheral blood and other tissues have been separation. CD133-positive stem cells have Pluripotency properties and are powerful candidates for application in cell therapy.
Materials & Methods: In this experimental study, MACS kit was used to isolate CD133+ cells of umbilical cord blood. CD 133 positive cells were evaluated by flowcytometry for surface stem cell marker such as CD 90, CD34, and CD105 and than karyotiping are performed.
Finding: Flowcytometry results showed that approximately 95% of purified cells were CD133+. Heterogeneous cell populations have positive level of CD105: 78.35, CD90: 82.32 and then CD34: 8.56. In CD133 positive fraction, expression of surface marker was: CD105: 55.63, CD90:43.25, CD34: 18.2.This cells were Normal karyotype and Spindle shaped like Mesenchymal stem cell.
Conclusion: The study results showed that the mentioned CD133 positive cells combination and alone can be act a Nominate as a valuable source to tissue engineering.
Keywords: |
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| Keywords: CD133+ cells, USSC, Flowcytomtry, Stem cell |
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Full-Text [PDF 648 kb]
(5451 Downloads)
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Type of Study: Research |
Subject:
Medical microbiology
Received: 2013/03/18 | Accepted: 2013/05/13 | Published: 2013/05/13
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