[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 7 (2-2014) ::
Journal of Ilam University of Medical Sciences 2014, 21(7): 34-42 Back to browse issues page
Cytotoxic effects of 5 - fluorouracil on Leishmania major promastigotes and induction of apoptosis in the parasite
Mahdi Delavari1, Abdolhossein Dalimi *2, Fatemeh Ghaffarifar1, Javid Sadraei1
1- Tarbiat modares university
2- Tarbiat modares university , : dalimi_a@modares.ac.ir
Abstract:   (12069 Views)
Introduction : Cutaneous leishmaniasis is a public health problem in Iran. Actually Antimony compounds commonly used in the treatment of this disease. Using these drugs associated with limitations and several side effects. Research for introducing a new and effective drug is considere. In the present study, the effect of 5 - fluorouracil on growth of Leishmania major promastigotes were evaluated in vitro conditions. In addition the effect of fluorouracil on induction of apoptosis in promastigotes of the leishmania major were investigated. Material and Methods: Different concentrations of 5–fluorouracil(12, 25, 50 and 100 µg/ml) were tested in three times and the IC50 was calculated by counting the number of parasites. Viability percent of promastigotes was determined after adding drug by MTT assay. Flow cytometry was used to investigate the induction of apoptosis in the parasites. Findings: The 5–fluorouracil has inhibitory effects on the growth of Leishmania promastigotes.IC50 was measured 26.17 µg/ml after 24h. Analysis of the results of flow cytometry showed that the 5 - fluorouracil induces apoptosis in Leishmania major promastigotes. Discussion and Conclusion: The 5-fluorouracil has antileishmanial effects on leishmania major. So it can be suggested to evaluate in vivo condition against cutaneous leishmaniasis.
Keywords: Leishmania major, Apoptosis, 5- fluorouracil
Full-Text [PDF 442 kb]   (5335 Downloads)    
Type of Study: Research | Subject: medical parasitology
Received: 2013/03/16 | Accepted: 2013/11/19 | Published: 2014/01/29
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

delavari M, Dalimi A, Ghaffarifar F, Sadraei J. Cytotoxic effects of 5 - fluorouracil on Leishmania major promastigotes and induction of apoptosis in the parasite. Journal of Ilam University of Medical Sciences 2014; 21 (7) :34-42
URL: http://sjimu.medilam.ac.ir/article-1-912-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 7 (2-2014) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.14 seconds with 31 queries by YEKTAWEB 4541