[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019

Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
Registered in



:: Volume 20, Issue 4 (2-2013) ::
Journal of Ilam University of Medical Sciences 2013, 20(4): 176-181 Back to browse issues page
Isoelectric Focusing Optimization of Female Mouse Brain Protein Extract
A Meyfour * 1, N Ahmadi , F Maleki
1- , meyfour_a@yahoo.com
Abstract:   (12421 Views)
Introduction: Brain (central nervous syst-em) is responsible to manage all biological process in living organisms, particularly mammalian. Since proteins play major roles in the biological cell activities, nowadays identification of brain proteins and deter-mination of their function is interesting subject for neurologists. Isoelectric Foc-using (IEF) technique is one of the prote-omic technologies to study brain proteins. This study was carried out to select the IPG strips with maximum resolution and to optimize IEF condition for the separation of mouse brain proteins. Materials & Methods: After extraction of mouse brain proteins and determination of protein concentration by Bradford assay, Isoelectric Focusing was performed using IPG strips with three different pH gradients. Findings: IPG strip with pH=3-10NL was able to separate more proteins with relati-vely high resolution in comparison with IPG strips with pH=3-10 and pH=4-7. Discussion & Conclusion: Results were shown that more proteins can be separated using IPG strip with pH=3-10 NL and this strip has a high resolution rather than the other strips. Due to the complexity of brain, its function, and the extent of brain proteins, it is appropriate to resolve proteins by the method.
Keywords: Mouse, Protein, Isoelectric fo-cusing, Brain
Full-Text [PDF 176 kb]   (4123 Downloads)    
Type of Study: Research | Subject: biology
Received: 2013/03/13 | Accepted: 2013/03/17 | Published: 2013/03/17
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

meyfour A, ahmadi N, maleki F. Isoelectric Focusing Optimization of Female Mouse Brain Protein Extract. J. Ilam Uni. Med. Sci. 2013; 20 (4) :176-181
URL: http://sjimu.medilam.ac.ir/article-1-907-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 4 (2-2013) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.18 seconds with 41 queries by YEKTAWEB 4646