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:: Volume 32, Issue 2 (6-2024) ::
Journal of Ilam University of Medical Sciences 2024, 32(2): 105-115 Back to browse issues page
Molecular epidemiology of Candida species isolated from clinical specimens and evaluation of their antifungal susceptibility testing to ketoconazole and nystatin with a review of studies
Niusha Kaveh1 , Ayatollah Nasrolahi omran * 2, Mohammad Taghi Hedatyati3 , Mojtaba Taghizade armaki4 , Mahdi Abastabara3 , Saeed Mahdavi Omran64
1- Dept of mycology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
2- Dept of mycology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran , ayat51@yahoo.co.in
3- Invasive Fungi Research Center, Communicable Diseases Institute, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran & Dept of Medical mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
4- Dept of Parasitology and Mycology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
Abstract:   (500 Views)
Introduction:  Candidiasis is a fungal infection caused by Candida yeasts in immunocompromised patients. This study aimed to assess antifungal susceptibility of clinical isolates of Candida species to ketoconazole and nystatin by broth microdilution.
Material & Methods:  In this descriptive-analytical study, 377 clinical isolates of Candida species were collected from different regions of Iran. The species were identified using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. The antifungal susceptibility testing of clinical isolates of Candida species to ketoconazole and nystatin was performed using broth microdilution according to standard protocol CLSI (M27-A3 and M27-S4). The standard strain of Candida parapsilosis was used as the quality of control. The minimum inhibitory of concentrations was determined after 24h at 37 ºC.
Results: Based on molecular identification, Candida albicans 266 (70.7%), C. krusei 64 (17%), C. parapsilosis 40 (10.7%), and C. tropicalis 6 (1.6%) were identified. The MIC90 and MIC50 values of ketoconazole and nystatin for all isolates were 0.063 µg/ml, 0.031 µg/ml, 2 µg/ml, and 4 µg/ml, respectively.
Discussion & Conclusion: As evidenced by the results of this study, antifungal susceptibility testing along with the identification of fungi at the species level would certainly be very useful in selecting primary antifungal agents for treatment, especially in invasive fungal infections. According to our findings, ketoconazole was much more effective on Candida spp. than nystatin.
Keywords: Antifungal susceptibility testing, Candida, Molecular identification
Full-Text [PDF 1293 kb]   (227 Downloads)    
Type of Study: Research | Subject: mycology
Received: 2023/11/23 | Accepted: 2024/01/27 | Published: 2024/06/4
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kaveh N, Nasrolahi omran A, Hedatyati M T, Taghizade armaki M, Abastabara M, Mahdavi Omran6 S. Molecular epidemiology of Candida species isolated from clinical specimens and evaluation of their antifungal susceptibility testing to ketoconazole and nystatin with a review of studies. J. Ilam Uni. Med. Sci. 2024; 32 (2) :105-115
URL: http://sjimu.medilam.ac.ir/article-1-8152-en.html


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Volume 32, Issue 2 (6-2024) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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