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Effects of Aluminum on Cultured Human Embryonic Astrocytes and Apoptotic Gene Expression
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Abstract: (12854 Views) |
| Abstract
Introduction: Aluminum is the third most abundant element in the earth's crust .It plays a role in several neurodegenerative conditions including Alzheimer's disease. In this study, we investigated the effects of aluminum on human embryonic astrocytes.
Materials & methods: In this experimental study, we applied MTT techniques to investigate the effects of 3,9, and 12 days exposure to aluminum on astrocyte viability . Then, we used immunocytochemical techniques to identify apoptotic gene expression changes induced by aluminum. We used Stata software to analyze the data.
Findings: Our results showed that 3-days exposure to 1 and 1.5 mM caused a reduction in astrocytes viability, (P<0.01, P<0.03). Low levels of aluminum (500 and 100 µM) needed long-term exposure to become toxic to astrocytes, (9 days for 500 and 12 days for 100 µM) (P=0.000). Aluminum didn't show any effects on apoptotic gene expression.
Conclusion: Long-term exposure, even to low levels of aluminum, was toxic for human embryonic astrocytes, but it seems that aluminum does not alter apoptotic gene expression. |
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| Keywords: aluminum, astrocyte, human embryo, cytotoxicity |
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Full-Text [PDF 346 kb]
(2919 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2009/02/18 | Accepted: 2015/04/20 | Published: 2015/04/20
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