Association Study of Polymorphisms in the LEP Gene with Breast Cancer Risk

Hamta, Ahmad; Ghasemian Safaei , Paniz

[Home ] [Archive]

[ فارسی ]

Journal of Ilam University of Medical Sciences

Main Menu

Home

Journal Information

Articles archive

Publication Ethics

Peer Review Process

Indexing Databases

For Authors

For Reviewers

Subscription

Contact us

Site Facilities

Google Scholar Metrics

	All	Since 2019
Citations	6870	4104
h-index	28	22
i10-index	206	99

Search in website

Receive site information

Registered in

Volume 32, Issue 4 (9-2024)

Journal of Ilam University of Medical Sciences 2024, 32(4): 42-52

Back to browse issues page

Association Study of Polymorphisms in the LEP Gene with Breast Cancer Risk

Ahmad Hamta ^*

¹, Paniz Ghasemian Safaei²

1- Dept of Biology, Faculty of Sciences, Arak University, Arak, Iran , a-hamta@araku.ac.ir
2- Dept of Biology, Faculty of Sciences, Arak University, Arak, Iran

Abstract: (231 Views)

Introduction: According to the conducted studies, breast cancer is one of the most common malignant tumors, and its incidence rate is increasing yearly. One of the measures to reduce the mentalities from breast cancer is early diagnosis of biomarkers, such as Leptin polymorphisms (rs7799039 and rs2167270), and timely treatment. Leptin is an adipocytokine made by fat cells, playing a crucial role in cell proliferation, survival, migration, and immune response. The present study aimed to assess the association between polymorphisms in the Leptin gene and breast cancer risk in Arak, Iran.
Materials & Methods: Two SNPs of the Leptin gene (rs7799039 and rs2167270) were genotyped by PCR-RFLP method (polymerase chain reaction-restriction fragment length polymorphism) in a case-control study, including 80 breast cancer patients and 80 healthy controls. In this study, all statistical analyses were performed in SPSS software (version 26) using the Chi-Squared test at P˂ 0.05 significant level.
Results: Based on the results, rs7799039 and rs2167270 showed no significant association with breast cancer risk (P=0.183 and P=0.86, respectively). In this study, the mean age in the patient group was 47 ( an age range of 28-79 years).
Conclusion: As evidenced by the obtained results, Leptin gene polymorphisms (rs7799039 and rs2167270) did not increase the risk of breast cancer in the Arak population in Iran. Although based on the present study results, there was no significant association between Leptin gene polymorphisms and breast cancer risk, according to the prominent role of the Leptin gene, the polymorphisms of this gene could be used as biomarkers to predict breast cancer in other populations.

Keywords: Breast cancer, LEP Gene, PCR-RFLP, Single nucleotide polymorphism

Full-Text [PDF 1499 kb] (133 Downloads)

Type of Study: Research | Subject: research
Received: 2023/07/9 | Accepted: 2024/05/21 | Published: 2024/09/22

References

1. Zhang YN, Xia KR, Li CY, Wei BL, Zhang B. Review of Breast Cancer Pathologigcal Image Processing. Biomed Res Int 2021;2021:1994764. doi: 10.1155/2021/1994764.

2. Flores-Dorantes MT, Díaz-López YE, Gutiérrez-Aguilar R. Environment and Gene Association With Obesity and Their Impact on Neurodegenerative and Neurodevelopmental Diseases. Front Neurosci 2020;14:863. doi: 10.3389/fnins.2020.00863.

3. Koerber-Rosso I, Brandt S, von Schnurbein J, Fischer-Posovszky P, Hoegel J, Rabenstein H, et al. A fresh look to the phenotype in mono-allelic likely pathogenic variants of the leptin and the leptin receptor gene. Mol Cell Pediatr 2021;8:10. doi:10.1186/s40348-021-00119-7.

4. Chun KA, Kocarnik JM, Hardikar SS, Robinson JR, Berndt SI, Chan AT, et al. Leptin gene variants and colorectal cancer risk: Sex-specific associations. PLoS One 2018;13:e0206519. doi: 10.1371/journal.pone.0206519.

5. Jiménez-Cortegana C, Hontecillas-Prieto L, García-Domínguez DJ, Zapata F, Palazón-Carrión N, Sánchez-León ML, et al. Obesity and Risk for Lymphoma: Possible Role of Leptin. Int J Mol Sci 2022;23:15530. doi:10.3390/ijms232415530.

6. Mahmoud R, Kimonis V, Butler MG. Genetics of Obesity in Humans: A Clinical Review. Int J Mol Sci 2022;23:11005. doi: 10.3390/ijms231911005.

7. Meshkani R, Nasimian A, Taheripak G, Zarghooni M, Rezaei M, Sadeghi A, et al. Association between Leptin G2548A and Leptin Receptor Q223R Polymorphisms with Type 2 Diabetes in an Iranian Population. Clin Lab 2016;62:89-96. doi:10.7754/clin.lab.2015.150535.

8. Pan WW, Myers MG Jr. Leptin and the maintenance of elevated body weight. Nat Rev Neurosci 2018;19:95-105. doi: 10.1038/nrn.2017.168.

9. Kargasheh FB, Ansaripour S, Borumandnia N, Moradi N, Zandieh Z, Maleki M, et al. Association of leptin G2548A and leptin receptor Q223R polymorphisms and their serum levels with infertility and recurrent pregnancy loss in Iranian women with polycystic ovary syndrome. PLoS One 2021;16:e0255920. doi: 10.1371/journal.pone.0255920.

10. Zhang L, Yuan Q, Li M, Chai D, Deng W, Wang W. The association of leptin and adiponectin with hepatocellular carcinoma risk and prognosis: a combination of traditional, survival, and dose-response meta-analysis. BMC Cancer 2020;20:1167. doi: 10.1186/s12885-020-07651-1.

11. Boubertakh B, Silvestri C, Di Marzo V. Obesity: The Fat Tissue Disease Version of Cancer. Cells 2022;11:1872. doi: 10.3390/cells11121872.

12. Katsiki N, Mikhailidis DP, Banach M. Leptin, cardiovascular diseases and type 2 diabetes mellitus. Acta Pharmacol Sin 2018;39:1176-88. doi: 10.1038/aps.2018.40.

13. Wang Y, Hu C. Leptin and Asthma: What Are the Interactive Correlations? Biomolecules 2022;12:1780. doi: 10.3390/biom12121780.

14. Du M, Wang Y, Vallis J, Shariati M, Parfrey PS, Mclaughlin JR, et al. Associations between polymorphisms in leptin and leptin receptor genes and colorectal cancer survival. Cancer Biol Med 2023;20:438-51. doi: 10.20892/j.issn.2095-3941.2022.0635.

15. Socol CT, Chira A, Martinez-Sanchez MA, Nuñez-Sanchez MA, Maerescu CM, Mierlita D, et al. Leptin Signaling in Obesity and Colorectal Cancer. Int J Mol Sci 2022;23:4713. doi: 10.3390/ijms23094713.

16. Tang W, Kang M, Liu C, Qiu H. Leptin rs7799039 (G2548A) polymorphism is associated with cancer risk: a meta-analysis involving 25,799 subjects. Onco Targets Ther 2019;12:2879-90. doi: 10.2147/OTT.S190093.

17. Karam HA, Bessa SS, Ali EMM, Diab T, Mohamed TM. The Inter-Relation between Leptin Receptor (Q223R) Gene Polymorphism and the Risk of Egyptian Patients with HCC. Asian Pac J Cancer Prev 2020;21:3557-65. doi: 10.31557/APJCP.2020.21.12.3557.

18. Atoum MF, Hamaid Alparrey AA. Association of Leptin Receptor Q223R Gene Polymorphism and Breast Cancer Patients: A Case Control Study. Asian Pac J Cancer Prev 2022;23:177-82. doi: 10.31557/APJCP.2022.23.1.177.

19. Li L, Meng X, Liu L, Xiang Y, Wang F, Yu L, et al. Single-Nucleotide Polymorphisms in LEP and LEPR Associated With Breast Cancer Risk: Results From a Multicenter Case-Control Study in Chinese Females. Front Oncol 2022;12:809570. doi: 10.3389/fonc.2022.809570.

20. Zhang A, Wang S, Zhang F, Li W, Li Q, Liu X. The Prognosis of Leptin rs2167270 G > A (G19A) Polymorphism in the Risk of Cancer: A Meta-Analysis. Front Oncol 2021;11:754162. doi: 10.3389/fonc.2021.754162.

21. Hao JQ, Zhang QK, Zhou YX, Chen LH, Wu PF. Association between circulating leptin concentration and G-2548A gene polymorphism in patients with breast cancer: a meta-analysis. Arch Med Sci 2019;15:275-83. doi: 10.5114/aoms.2018.75638.

22. Karakus N, Kara N, Ulusoy AN, Ozaslan C, Tural S, Okan I. Evaluation of CYP17A1 and LEP Gene Polymorphisms in Breast Cancer. Oncol Res Treat 2015;38:418-22. doi: 10.1159/000438940.

23. Luan H, Zhang H, Li Y, Wang P, Cao L, Ma H, et al. Association of two obesity-related gene polymorphisms LEPG2548A rs7799039 and LEPRQ223R rs1137101 with the risk of breast cancer. Oncotarget 2017;8:59333-44. doi: 10.18632/oncotarget.19580.

24. Mahmoudi R, Noori Alavicheh B, Nazer Mozaffari MA, Fararouei M, Nikseresht M. Polymorphisms of Leptin (-2548 G/A) and Leptin Receptor (Q223R) Genes in Iranian Women with Breast Cancer. Int J Genomics 2015;2015:132720. doi: 10.1155/2015/132720.

25. Yuan XL, Xu ZP, Liu CR, Yan LP, Tao P, Xiong P, et al. Study of the association between polymorphism of persistent obesity, human leptin gene/leptin receptor gene and molecular subtypes of breast cancer. Zhonghua Yu Fang Yi Xue Za Zhi 2017;51:533-8. doi: 10.3760/cma.j.issn.0253-9624.2017.06.015.

26. Yan W, Ma X, Gao X, Zhang S. Association Between Leptin (-2548G/A) Genes Polymorphism and Breast Cancer Susceptibility: A Meta-Analysis. Medicine (Baltimore) 2016;95:e2566. doi: 10.1097/MD.0000000000002566.

Send email to the article author

Add your comments about this article

Ethics code: IR.ARAKMU.REC.1399.294

Mendeley

Zotero

RefWorks

Hamta A, Ghasemian Safaei P. Association Study of Polymorphisms in the LEP Gene with Breast Cancer Risk. J. Ilam Uni. Med. Sci. 2024; 32 (4) :42-52
URL: http://sjimu.medilam.ac.ir/article-1-8031-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 32, Issue 4 (9-2024)

Back to browse issues page

Persian site map - English site map - Created in 0.15 seconds with 41 queries by YEKTAWEB 4671