Study of Micro RNA(miR-23a-3p) expression level in maternal plasma of women with early Recurrent miscarriage (RM) and Recurrent Implantation Failure(RIF)

Tutunfroush, Mina; Mohseni , Jafar; Ghorbian , Saeed; Danaii , Shahla; Ghiyamirad , Mehdi

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Volume 31, Issue 5 (12-2023)

Journal of Ilam University of Medical Sciences 2023, 31(5): 24-32

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Study of Micro RNA(miR-23a-3p) expression level in maternal plasma of women with early Recurrent miscarriage (RM) and Recurrent Implantation Failure(RIF)

Mina Tutunfroush¹

, Jafar Mohseni²

, Saeed Ghorbian ^*

³, Shahla Danaii³

, Mehdi Ghiyamirad⁴

1- Dept of molecular genetics, Ahar Branch, Islamic Azad University, Ahar,Iran
2- Genetics Research Div, ART Center of ACECR East Azerbaijan Branch, East Azerbaijan ACECR, Tabriz, Iran
3- Dept of Gynecology, ART center of ACECR East Azarbaijan Branch, East Azarbaijan ACECR, Tabriz, Iran
4- Dept of Microbiology, Ahar Branch, Islamic Azad University, Ahar,Iran

Abstract: (424 Views)

Introduction: Recurrent Pregnancy Loss and Recurrent Implantation Failure are major limiting factors in the establishment of pregnancy. Recurrent Pregnancy loss is defined as the failure of two or more clinically recognized pregnancies. A considerable proportion of infertile couples undergoing IVF treatment experiencing recurrent implantation failure (RIF) fail repeatedly to implant following at least three IVF cycles. MicroRNAs (miRNAs) can serve as reliable non-invasive diagnostic and prognostic biomarkers for pregnancy-related complications. Therefore, this study aimed to quantify miR-23a-3p expression level in the plasma of patients with idiopathic recurrent pregnancy loss (iRPL) and recurrent implantation failure compared to healthy subjects to evaluate its potential diagnostic value in iRPL and RIF patients.
Material & Methods: This study is a cross-sectional descriptive study. A total of 120 plasma samples were obtained from 40 women with a history of at least two consecutive iRPL, 40 women with RIF, and 40 healthy women without a history of miscarriage to evaluate the expression level of the circulating miR-23a-3p by quantitative real-time polymerase chain reaction (qPCR) technique. All participants were recruited from Assisted Reproductive Technology (ART) and Stem Cell Research Center, Tabriz, Iran, September 2019-March 2020. Statistical analysis was performed using SPSS (version 22). Diagnostic efficiency of the circulating miRNAs was determined by Receiver Operatig Characteristic curve analysis using Graphpad Prism version 9.1.1.
Findings: Our results showed that the miR-23a-3p expression level in plasma of iRPL patients was lower than those in healthy controls but without a statistically significant difference (P=0.113). We also found that miR-23a-3p plasma level in patients with RIF tended to be lower compared to healthy participants; however, it was not statistically significant (P=0.974).
Discussion & Conclusion: The current study provides evidence indicating that downregulation of miR-23a-3p may be associated with iRPL and RIF. Therefore, further research is needed, such as using different geographic regions, large sample sizes, and other techniques (microarray).

Keywords: Implantation, Micro RNA, Real Time PCR, Recurrent miscarriage

Full-Text [PDF 702 kb] (196 Downloads)

Type of Study: Research | Subject: departmet of gynecology and obstetrics
Received: 2023/01/3 | Accepted: 2023/08/19 | Published: 2023/12/6

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Tutunfroush M, Mohseni J, Ghorbian S, Danaii S, Ghiyamirad M. Study of Micro RNA(miR-23a-3p) expression level in maternal plasma of women with early Recurrent miscarriage (RM) and Recurrent Implantation Failure(RIF). J. Ilam Uni. Med. Sci. 2023; 31 (5) :24-32
URL: http://sjimu.medilam.ac.ir/article-1-7841-en.html

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