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:: Volume 31, Issue 2 (6-2023) ::
Journal of Ilam University of Medical Sciences 2023, 31(2): 31-41 Back to browse issues page
Preparation and Biological Investigation of Iron Magnetic Nanoparticles Coated with Chitosan/Alginate Biopolymer (Fe3O4/Alg/CS)
Elham Rostami * 1, Elham Hovazi2
1- Dept of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran , elhamrostami74@gmail.com
2- Dept of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (579 Views)
Introduction: Today, cancer is one of the health concerns in modern societies. The use of nanoparticles in diagnosis, drug delivery, imaging, and cancer treatment has received much attention in medical sciences.   The most important problem when treating cancer with chemotherapy is the lack of access to the central parts of the mass due to its less blood supply. This study aimed to investigate the toxicity of iron oxide nanoparticles coated with biopolymer chitosan/alginate on melanoma cancer cells (Hep G2 cells).
Material & Methods: In this research, magnetic iron nanoparticles were coated with two biopolymers, namely chitosan and alginate. The size and surface morphology of these nanoparticles were checked by size measuring device and scanning electron microscope. Moreover, the binding of functional groups of chitosan and alginate to iron magnetic nanoparticles was checked by an infrared spectrometer. In this study, magnetic iron nanoparticles and modified nanoparticles were treated for 24 hours and the IC50 concentration of the compounds was estimated. The toxic properties of these nanoparticles were evaluated by MTT test and acridine orange/ethidium bromide staining.
Findings: After examining the photos of the scanning electron microscope and the size measuring device, the size of 50 nanometers was shown for the modified iron nanoparticles, and the shape of these nanoparticles was observed to be completely round and spherical.
Discussion & Conclusion: The findings from the investigations of binanoparticles definitely confirmed the effective coating of nanoparticles by chitosan and alginate biopolymers. Furthermore, the findings showed that magnetic iron nanoparticles had higher toxic effects depending on the concentration and their IC50 concentration was about 134 µM/ml, while the coated nanoparticles had significantly lower toxic effects and did not have significant toxicity on Hep G2 cells at concentrations below 25 μM/ml. The coating of iron oxy nanoparticles significantly reduces their toxicity concentration.
Keywords: Biopolymer, Cell toxicity, Chitosan, Drug Delivery, Magnetic nanoparticles
Full-Text [PDF 1747 kb]   (295 Downloads)    
Type of Study: Research | Subject: biotechnolohgy
Received: 2022/08/17 | Accepted: 2023/01/17 | Published: 2023/06/5
References
1. Fatahian S, Shahbazi-Gahrouei D, Pouladian M, Yousefi M, Amiri G, Noori A. Biodistribution and toxicity assessment of radiolabeled and DMSA coated ferrite nanoparticles in mice. J Radioanal Nucl Chem 2012;293:915-21.doi: /10.1007/s10967-012-1822-y.
2. Shahbazi-Gahrouei D, Khaniabadi PM, Khaniabadi BM, Shahbazi-Gahrouei S. Medical imaging modalities using nanoprobes for cancer diagnosis: A literature review on recent findings. J Res Med Sci 2019; 24 :38. doi: 10.4103/jrms.JRMS_437_18.
3. Shahbazi-Gahrouei D, Khaniabadi PM, Shahbazi-Gahrouei S, Khorasani A, Mahmoudi F. A literature review on multimodality molecular imaging nanoprobes for cancer detection. Pol J Med Phys 2019;25:57-68. doi:10.2478/pjmpe-2019-0009.
4. Pankhurst Q, Thanh N, Jones S, Dobson J. Progress in applications of magnetic nanoparticles in biomedicine. J Phys D Appl Phys 2009;42. doi: 10.1088/00223727/42/22/224001.
5. Bakhtiary Z, Saei AA, Hajipour MJ, Raoufi M, Vermesh O, Mahmoudi M. Targeted superparamagnetic iron oxide nanoparticles for early detection of cancer: Possibilities and challenges. Nanomedicine: NBM 2016;12:287-307. doi:10.1016/j.nano.2015.10.019.
6. Khaniabadi PM, Shahbazi-Gahrouei D, Majid AMSA, Jaafar MS, Khaniabadi BM, Shahbazi-Gahrouei S. In vitro study of SPIONs-C595 as molecular imaging probe for specific breast cancer (MCF-7) cells detection. Iran Biomed J 2017;21:360-8. doi: 10.18869/acadpub.ibj.21.6.360.
7. Haghshenas M, Hoveizi E, Mohammadi T, Kazemi Nezhad SR. Use of embryonic fibroblasts associated with graphene quantum dots for burn wound healing in Wistar rats. In Vitro Cell Dev Biol 2019;55:312-22. doi: 10.1007/s11626-019-00331-w.
8. Alavi M, Rai M. Antisense RNA, the modified CRISPR-Cas9, and metal/metal oxide nanoparticles to inactivate pathogenic bacteria. Cell Mol Biomed Rep 2021;1:52-9. doi:10.55705/cmbr.2021.142436.1014.
9. Alavi M, Rai M, Martinez F, Kahrizi D, Khan H, Rose Alencar De Menezes I, et al. The efficiency of metal, metal oxide, and metalloid nanoparticles against cancer cells and bacterial pathogens: different mechanisms of action. Cell Mol Biomed Rep 2022;2:10-21. doi:10.55705/cmbr.2022.147090.1023.
10. Hovazi E, Rostami E. Fabrication, characterization, and biological applications of TiO2 nanoparticles coated by chitosan. J Nanomed Res 2023;8:50-60.doi: 10.22034/nmrj.2023.01.005.
11. Veiseh O, Gunn JW, Zhang M. Design and fabrication of magnetic nanoparticles for targeted drug delivery and imaging. Adv Drug Deliv Rev 2010;62:284-304. doi:10.1016/j.addr.2009.11.002.
12. Gautier J, Allard-Vannier E, Munnier E, Soucé M, Chourpa I. Recent advances in theranostic nanocarriers of doxorubicin based on iron oxide and gold nanoparticles. J Control Release 2013;169:48-61. doi:10.1016/j.jconrel.2013.03.018.
13. Munnier E, Cohen-Jonathan S, Hervé K, Linassier C, Soucé M, Dubois P, et al. Doxorubicin delivered to MCF-7 cancer cells by superparamagnetic iron oxide nanoparticles: effects on subcellular distribution and cytotoxicity. J. Nanopart Res 2011;13:959-71.doi: 10.1007/s11051-010-0093-1.
14. Babaei E, Sadeghizadeh M, Hassan ZM, Feizi MAH, Najafi F, Hashemi SM. Dendrosomal curcumin significantly suppresses cancer cell proliferation in vitro and in vivo. Int Immunopharmacol 2012;12:226-34. doi:10.1016/j.intimp.2011.11.015.
15. Hossein-Beigi F, Fatahian S, Shahbazi-Gahrouei D. In-vitro toxicity assessment of polydopamine-coated and uncoated Fe3O4 nanoparticles in cell line B16-F10 (Melanoma Cell). J Isfahan Med Sch 1. Fatahian S, Shahbazi-Gahrouei D, Pouladian M, Yousefi M, Amiri G, Noori A. Biodistribution and toxicity assessment of radiolabeled and DMSA coated ferrite nanoparticles in mice. J Radioanal Nucl Chem 2012;293:915-21.doi: /10.1007/s10967-012-1822-y.
16. Unsoy G, Gunduz U. Targeted drug delivery via chitosan-coated magnetic nanoparticles. Nanostructures Drug Deliv 2017. 835-64. doi:10.1016/B978-0-323-46143-6.00026-9.
17. Ramezani MR, Ansari-Asl Z, Hoveizi E, Kiasat AR. Polyacrylonitrile/Fe (III) metal-organic framework fibrous nanocomposites designed for tissue engineering applications. Mater Chem Phys 2019;229:242-50. doi:10.1016/j.matchemphys.2019.03.031.
18. Liao N, Wu M, Pan F, Lin J, Li Z, Zhang D, et al. Poly (dopamine) coated superparamagnetic iron oxide nanocluster for noninvasive labeling, tracking and targeted delivery of adipose tissue-derived stem cells. Sci Rep 2016;6:1-13.doi:10.1038/srep18746.
19. Saedi Z, Hoveizi E, Roushani M, Massahi S, Hadian M, Salehi K. Synthesis, characterization, anticancer properties and theoretical study of asymmetrical Cd (II) N2-Schiff base complexes. J Mol Struct 2019;1176:207-216. doi:10.1016/j.molstruc.2018.08.072.
20. Gu X, Zhang Y, Sun H, Song X, Fu C, Dong P. Mussel-inspired polydopamine coated iron oxide nanoparticles for biomedical application. J Nanomater 2015;2015:3. doi:10.1155/2015/154592.
21. Ankamwar B, Lai T, Huang J, Liu R, Hsiao M, Chen C, et al. Biocompatibility of Fe3O4 nanoparticles evaluated by in vitro cytotoxicity assays using normal, glia and breast cancer cells. Nanotechnology 2010;21:075102. doi: 10.1088/0957-4484/21/7/075102.
22. Berry H, Wang B, Zhang Q. The Behavior of Magnetic Properties in the Clusters of 4d Transition Metals. Molecules 2018;23: 1896 . doi:10.3390/molecules23081896.
23. Cai W, Wan J. Facile synthesis of superparamagnetic magnetite nanoparticles in liquid polyols. J Colloid Interface Sci 2007;305:366-70. doi:10.1016/j.jcis.2006.10.023.
24. Calderón M, Quadir MA, Sharma SK, Haag R. Dendritic polyglycerols for biomedical applications. Adv Mater 2010;22:190-218. doi:10.1002/adma.200902144.
25. Moghimi SM, Hunter AC, Murray JC. Long-circulating and target-specific nanoparticles: theory to practice. Pharmacol Rev 2001;53:283-31. doi: 10.1038/s41598-023-32409-7.
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Rostami E, Hovazi E. Preparation and Biological Investigation of Iron Magnetic Nanoparticles Coated with Chitosan/Alginate Biopolymer (Fe3O4/Alg/CS). J. Ilam Uni. Med. Sci. 2023; 31 (2) :31-41
URL: http://sjimu.medilam.ac.ir/article-1-7687-en.html


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Volume 31, Issue 2 (6-2023) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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