Studying the Effect of Lactobacillus fermentum on Arc and CREB Genes Expression of Involved in The Memory of Alzheimer's Rats

Mogaddam, Elnaz; Tehranipur, Maryam; Nakhaei Moghaddam, Mahboobeh; Nejad Shahrokhabadi, Khadijeh

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Volume 31, Issue 3 (8-2023)

Journal of Ilam University of Medical Sciences 2023, 31(3): 36-46

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Studying the Effect of Lactobacillus fermentum on Arc and CREB Genes Expression of Involved in The Memory of Alzheimer's Rats

Elnaz Mogaddam¹

, Maryam Tehranipur¹

, Mahboobeh Nakhaei Moghaddam ^*

², Khadijeh Nejad Shahrokhabadi¹

1- Dept of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2- Dept of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran , mahboobe_nak@yahoo.com

Abstract: (954 Views)

Introduction: Alzheimer's disease is a common progressive neurogenerative disease that leads to dementia and destruction of brain cells, especially in areas related to learning and memory such as the hippocampus. The role of the Arc gene in synaptic flexibility and memory consolidation has been proven and its expression is strongly influenced by neuronal activity. The aim of this study was to investigate the effect of Lactobacillus fermentum on the expression of Arc (effective in synaptic flexibility and memory consolidation) and CREB (effective in stabilizing synaptic changes during learning) genes, involved in the memory of Alzheimer's rats.
Material & ethods: In this study that was conducted in 2019, 30 male Wistar rats were divided into 5 groups (control, Alzheimer and 3 groups as treatment). In the treatment groups, simultaneously with induction of Alzheimer by stereotaxic method with streptozotocin, supernatant of Lactobacillus fermentum PTCC 1744 in MRS broth with doses of 10⁸, 10⁷, and 10⁶ cfu/ml was injected intraperitoneally for 21 days. After RNA extraction from hippocampus samples, cDNA was synthesized and the expression of the genes was evaluated by real-time PCR and LinReg PCR software.
Findings: The data showed an increase in gene expression of Arc and CREB in the treatment groups with a dose of 10⁶ cfu/ml compared to the Alzheimer's group and the difference was significant (P <0.001).
Discussion & conclusion: Extracellular compounds of L. fermentum may inhibit the progression of neuronal lesions due to their antioxidant and anti-inflammatory effects and may be effective in improving Alzheimer's.

Keywords: Alzheimer, Arc, CREB, Lactobacillus fermentum

Full-Text [PDF 877 kb] (518 Downloads)

Type of Study: Research | Subject: physiology
Received: 2022/05/3 | Accepted: 2023/05/13 | Published: 2023/09/6

References

1. Hirtz D, Thurman DJ, Gwinn-Hardy K, Mohamed M, Chaudhuri AR, Zalutsky R. How common are the "common" neurologic disorders? Neurology 2007; 68:326-37. doi: 10.1212/01.wnl.0000252807. 38124.a3.

2. Mohammadzadeh E, Alipour F, Khallaghi B. Evaluation of spatial memory impairment after Intracerebroventricular streptozocin injection in adult rats. Shefaye Khatam 2014; 2: 40-5. (Persian). doi: 10.18869/acadpub.shefa.2.1.40.

3. Anand R, Gill KD, Mahdi AA. Therapeutics of Alzheimer's disease: Past, present and future. Neuropharmacology 2014;76 Pt A:27-50. doi: 10.1016/j.neuropharm.2013.07.004.

4. Alipour F, Karimzadeh F, Hasanzadeh G. P59: Triazine improved hippocampal injuries in animal model of Alzheimer’s disease. Shefaye Khatam 2015; 2: 109. (Persian).

5. Flavell SW, Greenberg ME. Signaling mechanisms linking neuronal activity to gene expression and plasticity of the nervous system. Annu Rev Neurosci 2008; 31:563-90. doi: 10.1146/annurev.neuro.31.060407.125631.

6. Miyashita T, Kubik S, Lewandowski G, Guzowski JF. Networks of neurons, networks of genes: an integrated view of memory consolidation. Neurobiol Learn Mem 2008; 89:269-84. doi: 10.1016/j.nlm.2007.08.012.

7. Tzingounis AV, Nicoll RA. Arc/Arg3.1: linking gene expression to synaptic plasticity and memory. Neuron 2006; 52:403-7. doi: 10.1016/j.neuron.2006.10.016.

8. Plath N, Ohana O, Dammermann B, Errington ML, Schmitz D, Gross C, et al. Arc/Arg3.1 is essential for the consolidation of synaptic plasticity and memories. Neuron 2006; 52:437-44. doi: 10.1016/j.neuron.2006.08.024.

9. Hall J, Thomas KL, Everitt BJ. Rapid and selective induction of BDNF expression in the hippocampus during contextual learning. Nat Neurosci 2000; 3:533-5. doi: 10.1038/75698.

10. Kim JY, Kwon JH, Ahn SH, Lee SI, Han YS, Choi YO, et al. Effect of probiotic mix (Bifidobacterium bifidum, Bifidobacterium lactis, Lactobacillus acidophilus) in the primary prevention of eczema: a double-blind, randomized, placebo-controlled trial. Pediatr Allergy Immunol 2010;21(2 Pt 2): e386-93. doi: 10.1111/j.1399-3038.2009.00958. x.

11. de Roos NM, Katan MB. Effects of probiotic bacteria on diarrhea, lipid metabolism, and carcinogenesis: a review of papers published between 1988 and 1998. Am J Clin Nutr 2000; 71: 405-11.doi: 10.1093/ajcn/71.2.405.

12. Burns AJ, Rowland IR. Anti-carcinogenicity of probiotics and prebiotics. Curr Issues Intest Microbiol 2000; 1: 13-24.

13. Mosavi SF, Rahnema M, Heydarieh N, Tajabadi Ebrahimi M. The effect of Iranian native Lactobacillus pentosus on healing of gastric ulcers in male Wistar rats. J Arak Uni Med Sci 2013; 16:81-90.

14. Di Domenico F, Barone E, Perluigi M, Butterfield DA. Strategy to reduce free radical species in Alzheimer's disease: an update of selected antioxidants. Expert Rev Neurother 2015; 15:19-40. doi: 10.1586/14737175.2015.955853.

15. Naomi R, Embong H, Othman F, Ghazi HF, Maruthey N, Bahari H. Probiotics for Alzheimer's Disease: A Systematic Review. Nutrients 2021; 14:20. doi: 10.3390/nu14010020.

16. Gül H, Özcelik S, Sagdic O and Certel M. Sourdough bread production with lactobacilli and S. cerevisiae isolated from sourdoughs. Process Biochem 2005; 40: 691-7. doi: 10.1016/j.procbio.2004.01.044.

17. Nandhini L, Rajasekar T, Sakthivel M, Deivasigamani B. Isolation of bacteriocin producing lactic acid bacteria from different soil samples. AJBPR 2011; 4: 280-90.

18. Rashid S, Hassanshahian M. Screening, Isolation and identification of lactic acid bacteria from a traditional dairy product of Sabzevar, Iran. Int J Enteric Pathog 2014; 2: 1-5. (Persian). doi: 10.17795/ijep18393.

19. Moghaddam E, Tehranipour M, Nakhaie Moghadam M. Effect of probiotic prophylaxis of Lactobacillus fermentum on neuronal density of hippocampus in CA3, CA2, and CA1 regions in rats with Alzheimer’s disease. Res Med 2021; 45 :17-23.

20. Alipour F, Oryan S, Sharifzadeh M, Karimzadeh F, Kafami L, Irannejad H. The neuroprotective effect of a triazine derivative in an Alzheimer’s rat model. Acta Med Iran 2015; 53: 8-16. (Persian)

21. Zavvari F, Karimzadeh F. A review on the Bbhavioral tests for learning and memory assessments in rat. Shafae Khatam 2017; 5: 110-24. doi: 10.18869/acadpub.shefa.5.4.110.

22. Ahmed T, Enam SA, Gilani AH. Curcuminoids enhance memory in an amyloid-infused rat model of Alzheimer's disease. Neuroscience 2010; 169:1296-306. doi: 10.1016/j.neuroscience.2010.05.078.

23. Zhang J, Ke KF, Liu Z, Qiu YH, Peng YP. Th17 cell-mediated neuroinflammation is involved in neurodegeneration of aβ1-42-induced Alzheimer's disease model rats. PLoS One 2013;8: e75786. doi: 10.1371/journal.pone.0075786.

24. Faherty CJ, Xanthoudakis S, Smeyne RJ. Caspase-3-dependent neuronal death in the hippocampus following kainic acid treatment. Brain Res Mol Brain Res 1999; 70:159-63. doi: 10.1016/s0169-328x (99)00143-6.

25. Shahbazi B, Narenji H. Comparison of four methods of DNA extraction from gram-negative and gram-positive bacteria. Zanko J Med Sci 2014; 15: 9-16. (Persian).

26. Herring A, Ambrée O, Tomm M, Habermann H, Sachser N, Paulus W, et al. Environmental enrichment enhances cellular plasticity in transgenic mice with Alzheimer-like pathology. Exp Neurol 2009; 216:184-92. doi: 10.1016/j.expneurol.2008.11.027.

27. Kalt W, Blumberg JB, McDonald JE, Vinqvist-Tymchuk MR, Fillmore SA, Graf BA, et al. Identification of anthocyanins in the liver, eye, and brain of blueberry-fed pigs. J Agric Food Chem 2008; 56: 705-12. doi:10.1021/jf 0719981.

28. Grönlund MM, Arvilommi H, Kero P, Lehtonen OP, Isolauri E. Importance of intestinal colonization in the maturation of humoral immunity in early infancy: a prospective follow up study of healthy infants aged 0-6 months. Arch Dis Child Fetal Neonatal Ed 2000; 83: F186–F192. doi:10.1136/fn.83.3. f186.

29. Gao L, Zhang Y, Sterling K, Song W. Brain-derived neurotrophic factor in Alzheimer's disease and its pharmaceutical potential. Transl Neurodegener 2022; 11:4. doi: 10.1186/s40035-022-00279-0.

30. Farshbaf MJ, Ghaedi K, Megraw TL, Curtiss J, Faradonbeh MS, Vaziri P, et al. Does PGC1α/FNDC5/BDNF elicit the beneficial effects of exercise on neurodegenerative disorders? Neuromol Med 2016;18: 1-15. doi:10.1007/s12017-015-8370-x.

31. Vojdani R, Zali. Probiotics and their mechanism of work prevention and treatment of human diseases. Res Med Sci 2003 27: 320-2. (Persian).

32. Li X, Wang Q, Hu X, Liu W. Current status of probiotics as supplements in the prevention and treatment of infectious diseases. Front Cell Infect Microbiol 2022; 12:789063. doi: 10.3389/fcimb.2022.789063.

33. Agahi A, Hamidi Gh, Salami M, Alinaghipour A, Daneshvar Kakhaki R, Soheili M. The effect of probiotic supplements on cognitive function in patients with primary and secondary Alzheimer. J Arak Uni Med Sci 2018; 20: 1-9. (Persian).

34. Benvenuti S, Saccardi R, Luciani P, Urbani S, Deledda C, Cellai I, et al. Neuronal differentiation of human mesenchymal stem cells: changes in the expression of the Alzheimer's disease-related gene seladin-1. Exp Cell Res 2006; 312:2592-604. doi: 10.1016/j.yexcr.2006.04.016.

35. Zarei B, Esmaili A, Moshtaghian S, Habibian S. The role of Arc gene in memory. Genet 3rd Millennium 2010; 8: 2106- 11. (Persian).

36. O'Hagan C, Li JV, Marchesi JR, Plummer S, Garaiova I, Good MA. Long-term multi-species Lactobacillus and Bifidobacterium dietary supplement enhances memory and changes regional brain metabolites in middle-aged rats. Neurobiol Learn Mem 2017; 144: 36-47. doi: 10.1016/j.nlm.2017.05.015.

37. Kobayashi Y, Kuhara T, Oki M, Xiao JZ. Effects of Bifidobacterium breve A1 on the cognitive function of older adults with memory complaints: a randomised, double-blind, placebo-controlled trial. Benef Microbes 2019; 10: 511-20. doi: 10.3920/BM2018.0170.

38. Singh AK, Pandey SK, Naresh Kumar G. Pyrroloquinoline quinone-secreting probiotic Escherichia coli Nissle 1917 ameliorates ethanol- induced oxidative damage and hyperlipidemia in rats. Alcohol Clin Exp Res 2014; 38: 2127-37. doi: 10.1111/acer.12456.

39. Romo-Araiza A, Gutierrez-Salmean G, Galvan EJ, Hernandez-Frausto M, Herrera-Lopez G, Romo- Parra H, et al. Probiotics and prebiotics as a therapeutic strategy to improve memory in a model of middle-aged rats. Front Aging Neurosci 2018; 10: 416. doi: 10.3389/fnagi.2018.00416.

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Mogaddam E, Tehranipur M, Nakhaei Moghaddam M, Nejad Shahrokhabadi K. Studying the Effect of Lactobacillus fermentum on Arc and CREB Genes Expression of Involved in The Memory of Alzheimer's Rats. J. Ilam Uni. Med. Sci. 2023; 31 (3) :36-46
URL: http://sjimu.medilam.ac.ir/article-1-7565-en.html

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