[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations62733583
h-index2719
i10-index18478

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 30, Issue 5 (12-2022) ::
Journal of Ilam University of Medical Sciences 2022, 30(5): 101-111 Back to browse issues page
Combined Effect of Aerobic Exercise and Crocin Supplementation on the Prevention of Myocardial Tissue Cell Death in Male Wistar Rats
Sepideh Poursadeghi1 , Majid Kashef1 , Fereshteh Shahidi1 , Elham Vosadi * 2
1- Dept of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran
2- Dept of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Shahrood University of Technology, Semnan, Iran , e.vosadi@yahoo.com
Abstract:   (593 Views)
Introduction: Oxidative stress-induced cell death is activated by free radicals. Exercise and nutrition appear to have the potential to modulate cell proliferation and death. This study aimed to evaluate the combined effect of aerobic exercise and crocin consumption on the prevention of myocardial tissue cell death in male Wistar rats.
Material & Methods: In this experimental study, 36 male Wistar rats with a mean weight of 180-200g were randomly divided into six groups (oxygenated water, oxygenated water+crocin, oxygenated water+ exercise, oxygenated water+crocin+exercise, sham, and control). After two weeks of familiarity with the environment and learning the exercises,  the training groups were given aerobic exercises for six weeks. All groups, except for the sham and control, were given 1 mM H2O2 and subcutaneously injected with 12.5 mg crocin. 48 hours after the last training session, the rats were anesthetized with CO2 after 10-12 hours of fasting, and their myocardial tissue was isolated. To measure the pro-apoptotic genes, Real-Time PCR was used; moreover, one-way analysis of variance and Scheffe post-Hoc test were employed for data analysis. A P-value of ≥0.05 was considered statistically significant.
(Ethic code: IR.SSRI.REC.1397.254)
Findings: The findings showed that six weeks of aerobic training in water, consumption of crocin supplementation, as well as the combination of aerobic exercise and crocin supplementation, reduced the expression of the genes of the internal apoptotic pathway at a significant level of ≥ 0.05.
Discussion & Conclusion: According to the results of the present study, it seems that the interaction and combination of aerobic exercise in water and crocin antioxidant supplement should be used to reduce the apoptosis of the myocardial tissue, less cell damage, and ultimately the health of the myocardial tissue.
Keywords: Aerobic exercise, Crocin supplement, Myocardial cell death, Pro-apoptotic genes
Full-Text [PDF 1046 kb]   (299 Downloads)    
Type of Study: Research | Subject: Physical Education
Received: 2021/11/14 | Accepted: 2022/05/14 | Published: 2022/12/6
References
1. Crist BL, Alekel DL, Ritland LM, Hanson LN, Genschel U, Reddy MB. Association of oxidative stress, iron, and centralized fat mass in healthy postmenopausal women. J Womens Health 2009;18:795-801. doi: 10.1089/jwh.2008.0988.
2. Young IS, McEneny J. Lipoprotein oxidation and atherosclerosis. Biochem Soc Trans 2001;29:358-62. doi: 10.1042/bst0290358.
3. Rinaldi B, Corbi G, Boccuti S, Filippelli W, Rengo G, Leosco D, et al. Exercise training affects age-induced changes in SOD and heat shock protein expression in rat heart. Exp Gerontol 2006;41:764-70. doi: 10.1016/j.exger. 2006.05.008
4. Haendeler J, Popp R, Goy C, Tischler V, Zeiher AM, Dimmeler S. Cathepsin D and H2O2 stimulate degradation of thioredoxin-1: implication for endothelial cell apoptosis. J Biol Chem 2005;280:42945-51. doi: 10.1074/jbc. M506985200.
5. Khalatbary AR. Apoptosis in neurodegenerative diseases. J Gorgan Uni Med Sci 2014;16:1-1. (persian)
6. Cerella C, Grandjenette C, Dicato M, Diederich M. Roles of apoptosis and cellular senescence in cancer and aging. Curr Drug Targets 2016;17:405-15. doi: 10.2174/13894501166661 50202155915
7. Phaneuf S, Leeuwenburgh C. Apoptosis and exercise. Med Sci Sports Exerc 2001;33:393-6. doi: 10.1097/00005768-200103000-00010.
8. Brentnall M, Rodriguez-Menocal L, De Guevara RL, Cepero E, Boise LH. Caspase-9, caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis. BMC cell biol 2013;14:1-9. doi: 10.1186/1471-2121-14-32.
9. Bezzerides V, Rosenzweig A. Saying yes to exercise and NO to cardiac injury. Circ Res 2011; 108:1414-16. doi: 10.1161/CIRCRESAHA.111.247122.
10. Ignarro LJ, Balestrieri ML, Napoli C. Nutrition. physical activity, and cardiovascular disease: an update. Cardiovasc Res 2007; 73:326-40. doi: 10.1016/j.cardiores.2006.06.030.
11. Haykowsky MJ. Left ventricular remodelling and the athlete's heart: time to revisit the Morganroth hypothesis. J Physiol 2011;589:5915-23. doi: 10.1113/jphysiol.2011.221903.
12. Mallikarjuna K, Shanmugam KR, Nishanth K, Wu MC, Hou CW, Kuo CH, et al. Alcohol-induced deterioration in primary antioxidant and glutathione family enzymes reversed by exercise training in the liver of old rats. Alcohol 2010;44:523-9. doi: 10.1016/j.alcohol.2010.07.004.
13. Huang CY, Yang AL, Lin FNW, Lin JA, Chan YS, Tsai FJ, et.al. Anti-apoptotic and pro-survival effects of exercise training on hypertensive hearts. J Appl Physiol 2011;112:883-91. doi: 10.1152/ japplphysiol.00605.2011.
14. Meredith-Jones K, Waters D, Legge M, Jones L. Upright water-based exercise to improve cardiovascular and metabolic health: a qualitative review. Complement Ther Med 2011;19:93-103. doi: 10.1016/j.ctim.2011.02.002.
15. Nakamoto H, Kaneko T, Tahara S, et al. Regular exercise reduces 8-oxodG in the nuclear and mitochondrial DNA and modulates the DNA repair activity in the liver of old rats. Exp Gerontol 2007; 42:287-95. doi: 10.1016/j. exger.2006.11.006.
16. Nayanatara A, Nagaraja H, Anupama B. The effect of repeated swimming stress on organ weights and lipid peroxidation in rats. Thai J Pharm Sci 2005;18:3-9.
17. Powers SK, Quindry JC, Kavazis AN. Exercise-induced cardioprotection against myocardial ischemia–reperfusion injury. Free Radic Biol Med 2008; 44:193-201. doi: 10.1016/j.freeradbiomed. 2007.02.006.
18. Habibi P, Alihemmati A, NourAzar A, Yousefi H, Mortazavi S, Ahmadiasl N. Expression of the Mir-133 and Bcl-2 could be affected by swimming training in the heart of ovariectomized rats. Iran J Basic Med Sci 2016;19:381-93. (persian) doi: 10.22038/ijbms.2016.6809.
19. Tanya RL, Christine M. Bone density and physical function in postmenopausal women after a 12-month water exercise intervention. Corval Oreg State Uni 2006; 541:9524-737.
20. No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, et al. Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 2020;472:179-93. doi: 10.1093/cvr/cvr015.
21. Olah A, Barta BA, Sayour AA, Ruppert M, Virág-Tulassay E, Novák J, et al. Balanced intense exercise training induces atrial oxidative stress counterbalanced by the antioxidant system and atrial hypertrophy that is not associated with pathological remodeling or arrhythmogenicity. Antioxidants 2021;10:452. doi: 10.3390/antiox 10030452.
22. Su QS, Tian Y, Zhang JG, Zhang H. Effects of allicin supplementation on plasma markers of exercise-induced muscle damage, IL-6 and antioxidant capacity. Eur J Appl Physiol 2008;103:275-83. doi: 10.1007/s00421-008-0699-5.
23. Keramati S. Effects of caraway supplementation on oxidative enzymatic stress markers in healthy women following aerobic exercise. 2012; M.A Thesis on Exercise Physiology. (persian)
24. Bjorklund G, Chirumbolo S. Role of oxidative stress and antioxidants in daily nutrition and human health. Nutrition 2017; 33:311-21. doi: 10.1016/j.nut.2016.07.018.
25. Hosseinzadeh H, Sadeghnia HR, Ziaee T, Danaee A. Protective effect of aqueous saffron extract (Crocus sativus L.) and crocin, its active constituent, on renal ischemia-reperfusion-induced oxidative damage in rats. J Pharm Pharm Sci. 2005;8:387-93. (persian)
26. Dianat M, Radan M. A Review on the Effective Properties of Crocin in the Management of Cardiopulmonary Dysfunction. Jundishapur J Physiol 2021; 2; 22-8. (persian)
27. Abdulkareem Aljumaily SA, Demir M, Elbe H, Yigitturk G, Bicer Y, Altinoz E. Antioxidant, anti-inflammatory, and anti-apoptotic effects of crocin against doxorubicin-induced myocardial toxicity in rats. Environ Sci Pollu Res 2021: 28:1-2. (persian) doi: 10.21203/rs.3.rs-418902/v1.
28. Moradi A, Hosseini SA, Nikbakht M. Effect of swimming training and crocin consumption on intrinsic apoptosis pathway in muscle tissue of high-fat diet-induced obese rats. Middle East J Rehabil Health Stud 2019: 31;6. (persian) doi: 10.5812/mejrh.92612.
29. Hassanpour G, Azarbayjani MA, Shakeri N, Abednazari H. The Effect of Interval and Continued Trainings with Crocin on Apoptotic Markers in the Heart Tissue of High-Fat Diet and Streptozotocin Induced Type 2 Diabetic Rats. Rep Health Care 2017: 1;3:58-70. (persian)
30. Rafiei MM, Gaeini A, Kordi MR, Nuri R. The Effect of Eight Weeks of Aerobic Exercise on Gene Expression of Cytochrome C, Caspase 9 and Tumor Volume in Mice with Breast Cancer. Rep Health Care 2018;4:55-60. (persian)
31. Radák Z, Sasvári M, Nyakas C, Pucsok J, Nakamoto H, Goto S. Exercise preconditioning against hydrogen peroxide-induced oxidative damage in proteins of rat myocardium. Arch biochem biophys 2000;376:248-51. (persian) doi: 10.1006/abbi.2000.1719.
32. Lari P, Abnous K, Imenshahidi M, Rashedinia M, Razavi M, Hosseinzadeh H. Evaluation of diazinon-induced hepatotoxicity and protective effects of crocin. Toxicol Ind Health 2015; 31:367-76. doi: 10.1177/0748233713475519.
33. Favaloro B, Allocati N, Graziano V, Di Ilio C, De Laurenzi V. Role of apoptosis in disease. Aging 2012;4:330. doi: 10.18632/aging.100459.
34. Gharekhanlo R, Mollahnoori M. Molecular Exercise Physiology: An Introduction. 1nd ed. Hatmi Publication 2014; p.115-4.
35. Czabotar PE, Lessene G, Strasser A, Adams JM. Control of apoptosis by the BCL-2 protein family: implications for physiology and therapy. Nat Rev Mol Cell Biol 2014;15:49-63.doi: 10.1038/ nrm3722.
36. Abadi N, Bashiri J. The effect of three-month aerobic training on the expression of AIF and caspase-9 gene in male rat soleus muscle. J Fasa Univ Med Sci 2017; 7:257-64. (persian)
37. Carraro U, Franceschi C. Apoptosis of skeletal and cardiac muscles and physical exercise. Aging 1997;9:19-34. doi: 10.1007/BF03340125.
38. Mooren F, Völker Klaus C. Molecular and cellular exercise Physiology. Translated by: Tartibian Bakhtiar and colleagues: University of Urmia unit 2012.
39. Pietrangelo T, Di Filippo ES, Mancinelli R, Doria C, Rotini A, Fanò-Illic G, et al. Low intensity exercise training improves skeletal muscle regeneration potential. Front Physiol 2015;6:399. doi: 10.3389/fphys.2015.00399.
40. Soori R, Ghram A, Zare Shahneh M, Choobineh S, Costa PB, Voltarelli FA. Effects of high intensity interval training and aging on cardiac muscle apoptosis markers in C57BL/6 Mice. Sport Sci Health 2021;17:173-9. doi: 10.1007/ s11332-020-00670-2.
41. Seyedgomi F, Bashiri J, Gholami F. Effect of High Intensity Endurance Training on p53 and Cytochrome-c Gene Expression in Male Rat Soleus Muscle. Armaghane-danesh 2017; 22: 608-22. (persian)
42. Xiang M, Qian ZY, Zhou CH, Liu J, Li WN. Crocetin inhibits leukocyte adherence to vascular endothelial cells induced by AGEs. J Ethnopharmacol 2006;107:25-31. doi: 10.1016/j. jep.2006.01.022.
43. Bjorklund G, Chirumbolo S. Role of oxidative stress and antioxidants in daily nutrition and human health. Nutrition 2017; 33:311-21. doi: 10.1016/j.nut.2016.07.018.
44. Xu GL, Qian ZY, Yu SQ, Gong ZN, Shen XC. Evidence of crocin against endothelial injury induced by hydrogen peroxide in vitro. J Asian Nat Produ Res 2006;8:79-85. doi: 10.1080/10286020500044732.
45. Lv B, Chen T, Xu Z, Huo F, Wei Y, Yang X. Crocin protects retinal ganglion cells against H2O2-induced damage through the mitochondrial pathway and activation of NF-κB. Int J Mol Med 2016;37:225-32. doi: 10.3892/ijmm.2015.2418.
46. Boussabbeh M, Salem IB, Belguesmi F, Neffati F, Najjar MF, Abid-Essefi S, et al. Crocin protects the liver and kidney from patulin-induced apoptosis in vivo. Environ Sci Pollut Res Int 2016;23:9799-808. doi: 10.1002/tox.22185.
47. Nayanatara AK, Nagaraja HS, Anupama BK. The effect of repeated swimming stress on organ weights and lipid peroxidation in rats. Thai J Pharm Sci 2005;18:3-9.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: IR.SSRI.REC.1397.254


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Poursadeghi S, Kashef M, Shahidi F, Vosadi E. Combined Effect of Aerobic Exercise and Crocin Supplementation on the Prevention of Myocardial Tissue Cell Death in Male Wistar Rats. J. Ilam Uni. Med. Sci. 2022; 30 (5) :101-111
URL: http://sjimu.medilam.ac.ir/article-1-7361-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 30, Issue 5 (12-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.15 seconds with 41 queries by YEKTAWEB 4643