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:: Volume 30, Issue 2 (6-2022) ::
Journal of Ilam University of Medical Sciences 2022, 30(2): 71-81 Back to browse issues page
Effects of Eight Weeks of High-Intensity Interval Training on the Expression of the Nkx2.5 and Tbx5 Genes in the Heart Tissue of Type 2 Male Diabetic Rats
Mahdieh Kousha1 , Hossein Abednatanzi * 2, Mandana Gholami1 , Farshad Ghazalian1
1- Dept of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Dept of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran , abednazari@gmail.com
Abstract:   (438 Views)
Introduction: Exercise and antioxidants consumption are known as protective effect against the risk of diabetes. Therefore, the current study aimed to investigate the effects of eight weeks of high-intensity interval training (HIIT) and thyme honey on the expression of Nkx2.5 and Tbx5 genes in the heart tissue of male type 2 diabetic rats.
Material & Methods: This experimental study was performed on 36 male diabetic rats which were randomly divided into four groups of control (n=8), HIIT (n=10), thyme honey (n=8), and HIIT+thyme honey (n=10). The HIIT training intervention was conducted in eight weeks (five sessions per week) and included 2 to 8 intervals with 2 min running at 80%-90% VO2max and 1 min at 50%-56% VO2max. Additionally, the supplement groups consumed 3 g/kg of thyme honey, 5 days/week. Both Nkx2.5 and Tbx5 gene expression in heart tissue were measured using real-time RT-PCR. To evaluate the differences among the groups, ANOVA and LSD post hoc tests were used at the significance level of P≤0.05.
(Ethic code: IR.SSRC.REC.1399.080)
Findings: The results revealed that the expression of the Nkx2.5 gene was significantly increased only in the HIIT group, compared to the control group (P=0.03); however, it was not observed in other intervention groups (P>0.05). The expression of the Tbx5 gene was significantly increased in both HIIT (P=0.02) and HIIT+thyme honey (P=0.02) groups, compared to the control group.
Discussion & Conclusion: The HIIT is associated with increased expression of the Nkx2.5 and Tbx5 genes in the heart tissue of diabetic rats; however, it can not be said exactly that thyme honey has similar effects in diabetic rats.
Keywords: Antioxidant, Diabetes, High-intensity interval training, Honey
Full-Text [PDF 1139 kb]   (164 Downloads)    
Type of Study: Research | Subject: Physical Education
Received: 2021/08/18 | Accepted: 2021/12/12 | Published: 2022/06/5
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Ethics code: IR.SSRC.REC.1399.080

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Kousha M, Abednatanzi H, Gholami M, Ghazalian F. Effects of Eight Weeks of High-Intensity Interval Training on the Expression of the Nkx2.5 and Tbx5 Genes in the Heart Tissue of Type 2 Male Diabetic Rats. sjimu 2022; 30 (2) :71-81
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Volume 30, Issue 2 (6-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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