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The histological effects of chronic multiple sequential stress on rat testis
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Maryam Fard1   , Farzad Rajaei * 2, Mohammad-Reza Sarokhani1 |
1- Qazvin University Of Medical Sciences
2- Qazvin University Of Medical Sciences , farzadraj@yahoo.co.uk |
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Abstract: (10805 Views) |
| Background: The increase of stress following the technological improvements appears to be an important factor that causes organs disorders like genital system. Therefore the present study was conducted to investigate the effects of chronic multiple sequential stress on rat testis
Methods: 18 Wistar rats were divided randomly into two equal groups. In animals under stress, the mice were exposed to different multiple sequential stress as Forced swimming, Restraint, Water deprivation, Isolation and Food deprivation for 10 days while the animals in control group were kept in their cages without any disorders. After weighing the animals and anesthesing with xylazine-Ketamine, the testis of animals were removed and weighed. After fixation with formaldehyde (10%), testis samples were prepared for light microscopic study. The number of spermatogonia, spermatocytes I & II, sperm & leydig cells, thickness of the seminiferous tubule determined using Image Tool software in the studied groups. Finally the data were compared statistically.
Results: The present study showed that the mean number of spermatogonia، spermatocyte I & II، sperm & leydig cells in stress group have significantly decreased compared to control group (P<0.001). Moreover, the mean thickness of seminiferous tubule in stress group have significantly decreased compared to control group (P<0.001)
Conclusion: Our study showed that chronic multiple sequential stress can have negative effects by reducing the number of spermatogonia, spermatocyte I & II, sperm, leydig cells & thickness of seminiferous tubule in rat testis but more studies are needed to confirm these results. |
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| Keywords: chronic multiple sequential stress, seminiferous tubule, spermatogonia, sperm, leydig, rat |
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Full-Text [PDF 257 kb]
(4168 Downloads)
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Type of Study: Research |
Subject:
physiology
Received: 2012/08/28 | Accepted: 2013/10/29 | Published: 2013/10/29
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