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:: Volume 30, Issue 1 (3-2022) ::
Journal of Ilam University of Medical Sciences 2022, 30(1): 95-103 Back to browse issues page
Effect of Aqueous Extract of Allium Canadense on the Activity of the Inhibited Acetylcholinesterase Enzyme by Organophosphate Diazinon
Mino Asadi1 , Faranak Hadi * 2, Seyed Hesamuddin Hejazi1 , Farideh Azarbani1
1- Dept of Biology, Faculty of Basic Sciences, Lorestan University, Khorramabad, Iran
2- Dept of Biology, Faculty of Basic Sciences, Lorestan University, Khorramabad, Iran , hadi.f@lu.ac.ir
Abstract:   (1616 Views)
Introduction: Allium genus with more than 900 important breeding and wild species, as a natural and bioactive antioxidant, is widely used in the pharmaceutical and food industries. This study aimed to investigate the effect of antioxidant activity of aqueous extract of Allium canadense and its effect on the inhibition of acetylcholinesterase enzyme and recovery of the inhibited enzyme activity.
Material & Methods: After the preparation of Allium canadense aqueous extract, the antioxidant activity of the extract at the concentrations of 100, 500, and 1000 μg/ml was investigated using DPPH free radical scavenging method. Following that, the activity of serum acetylcholinesterase and its reactivation after inhibition by organophosphate diazinon was evaluated at the concentrations of 100, 500, and 1000 μg/ml of the extract by the Ellman method.
Findings: Inhibition concentration of 50% (IC50) of free radicals of DPPH was 360/19 μg/ml.   Aqueous extract of Allium canadense did not inhibit acetylcholinesterase activity and was added to the concentration dependently. The regeneration of the inhibited enzymes activity was also observed in the vicinity of different concentrations of the aqueous extract of the plant.
Discussion & Conclusion: The aqueous extract of Allium canadense does not inhibit acetylcholinesterase activity; however, it can reactivate the enzyme inhibited by diazinon. The reason for this positive effect may be related to the high antioxidant activity in the extract.
 
Keywords: Allium canadense, Anti-acetylcholinesterase, Antioxidant activity, Aqueous extract, Organophosphate diazinon
Full-Text [PDF 1184 kb]   (733 Downloads)    
Type of Study: Research | Subject: biochemistry
Received: 2021/04/8 | Accepted: 2021/10/23 | Published: 2022/03/21
References
1. Liao J, Boschetti N, Mortensen V, Jensen SP, Koch C, Nørgaard-Pedersen B, et al. Characterization of salt‐soluble forms of acetylcholinesterase from bovine brain. J Neurochem. 1994; 63: 1446-1453.‌ doi: 10.1046/j.1471-4159.1994.63041446.x. PMID: 7931296
2. Carroll RT, Grimm JL, Hepburn TW, Emmerling MR. Purification of acetylcholinesterase by tacrine affinity chromatography. Protein Expr Purif. 1995; 6:389-93. doi: 10.1006/prep.1995.1051. PMID: 8527921.
3. Hebert LE, Scherr PA, Bienias JL, Bennett DA, Evans DA. Alzheimer disease in the US population: prevalence estimates using the 2000 census. Arch neurol. 2003;60:1119-1122. doi:10.1001/archneur.60.8.1119.
4. Arendt T, Brückner MK, Lange M, Bigl V. Changes in acetylcholinesterase and butyrylcholinesterase in Alzheimer's disease resemble embryonic development—a study of molecular forms. Neurochem int. 1992; 21: 381-396. doi: 10.1016/0197-0186(92)90189-X.
5. Britton NL, Brown A. An Illustrated Flora of the Northern United States: Canada and the British Possessions from Newfoundland to the Parallel of the Southern Boundary of Virginia, and from the Atlantic Ocean Westward to the 102d Meridian: C. Scribner's sons; 1913.
6. Stajner D, Igic R, Popovic B, Malencic D. Comparative study of antioxidant properties of wild growing and cultivated Allium species. Phytother Res. 2008; 22:113-117. doi: 10.1002/ptr.2278.
7. Ried K, Fakler P. Potential of garlic (Allium sativum) in lowering high blood pressure: mechanisms of action and clinical relevance. Integr Blood Press Control. 2014; 7:71. doi:10.2147/IBPC.S51434
8. Djurdjevic L, Dinic A, Pavlovic P, Mitrovic M, Karadzic B, Tesevic V. Allelopathic potential of Allium ursinum L. Biochem Syst Ecol. 2004; 32:533-544. doi:10.1016/j.bse.2003.10.001
9. Ogzewalla CD, Bonfiglio JF, Sigell LT. Common plants and their toxicity. Pediatr Clin N Am. 1987; 34:1557-1598. doi:10.1016/S0031-3955(16)36373-8.
10. Karami-Osboo R, Mirabolfathy M, Kamran R, Shetab-Boushehri M, Sarkari S. Aflatoxin B1 in maize harvested over 3 years in Iran. Food Control. 2012; 23:271-4. doi:10.1016/j.foodcont.2011.06.007
11. Jokanovic M. Medical treatment of acute poisoning with organophosphorus and carbamate pesticides. Toxicol lett. 2009;190 :2:107-15. doi: 10.1016/j.toxlet.2009.07.025.
12. Misra U, Nag D, Bhushan V, Ray P. Clinical and biochemical changes in chronically exposed organophosphate workers. Toxicol lett. 1985;24:187-193. doi: 10.1016/0378-4274(85)90056-6.
13. Husin L, Uttaman A, Hisham H, Hussain I, Jamil M. The effect of pesticide on the activity of serum cholinesterase and current perception threshold on the paddy farmers in the Muda agricultural development area, MADA, Kedah, Malaysia. Med j Malaysia. 1999; 54:320-324.
14. Kalender S, Ogutcu A, Uzunhisarcikli M, Açikgoz F, Durak D, Ulusoy Y, et al. Diazinon-induced hepatotoxicity and protective effect of vitamin E on some biochemical indices and ultrastructural changes. Toxicology. 2005; 211: 197-206. doi:10.1016/j.tox.2005.03.007
15. Ncir M, Saoudi M, Sellami H, Rahmouni F, Lahyani A, Makni Ayadi F, et al. In vitro and in vivo studies of Allium sativum extract against deltamethrin-induced oxidative stress in rats brain and kidney. Arch physiol biochem. 2018; 124: 207-217. doi:10.1080/13813455.2017.1376335
16. Sargazi Z, Nikravesh M, Jalali M, Sadeghnia H. The protective effect of vitamin E on serum and erythrocytes cholinesterase levels in poisoning of Diazinon, in adult female rats. Journal of North Khorasan Universtity of Medical Sciences 2016; 7:801-812. doi: 10.29252/jnkums.7.4.801. (Persian)
17. Shokrzade M, Habibi O, Abbasi A, Makhlugh M, Sadat Hosseini A, Poolaee S. Protective Effect of Methanol and Hot-water Extraction from Trametes versicolor on Ethanol-induced Liver Toxicity in Mice. J Mazandaran Univ Med Sci. 2015; 24:141-51. (Persian)
18. Malmir S and Jafari M. Comparison of the effects of diazinon and paraoxon on the antioxidant system of rat lung. Sci J Kurd Univ of Med Sci. 2014; 19: 123-133. (Persian)
19. Jafari M, Salehi M, Asgari A, Ahmadi S, Abbasnezhad M, Hajihoosani R. et al. Effects of paraoxon on serum biochemical parameters and oxidative stress induction in various tissues of Wistar and Norway rats. Environ toxicol and pharmacol. 2012; 34: 876-887. doi: 10.1016/j.etap.2012.08.011
20. Jafari M, Salehi M, Ahmadi S, Asgari A, Abasnezhad M, Hajigholamali M. The role of oxidative stress in diazinon-induced tissues toxicity in Wistar and Norway rats. Toxicol mech methods. 2012; 22: 638-647. doi: 10.3109/15376516.2012.716090.
21. Khazaie S, Jafari M, Heydari J, Salem F. Investigating response of spleen and erythrocytes antioxidant defense system on the effects of n-acetyl cysteine against Paraoxon toxicity in rat. Stud Med Sci. 2015; 26: 176-184. (Persian).
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Asadi M, Hadi F, Hejazi S H, Azarbani F. Effect of Aqueous Extract of Allium Canadense on the Activity of the Inhibited Acetylcholinesterase Enzyme by Organophosphate Diazinon. J. Ilam Uni. Med. Sci. 2022; 30 (1) :95-103
URL: http://sjimu.medilam.ac.ir/article-1-7053-en.html
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Volume 30, Issue 1 (3-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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