[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 29, Issue 4 (11-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(4): 74-82 Back to browse issues page
Evaluation of Grapefruit Juice in terms of Interleukin 18 Gene Expression in Rats with Fatty Liver and Healthy Rats
Simin Bahmanpoor1 , Noosha Zia-Jahromi * 2
1- Dept of Biology, Science Faculty, Falavarjan Branch, Islamic Azad University, Falavarjan, Iran
2- Dept of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , nooshazia.59@gmail.com
Abstract:   (811 Views)
Introduction: Interleukin 18 (IL-18) is a member of the cytokine family and an important regulator of inflammatory reactions. Due to the severe and irreversible complications of this disease in the advanced stages, timely treatment, especially with herbal medicines, can be considered an important strategy. This study aimed to investigate the effect of grapefruit extract on the IL-18 gene expression in mice with fatty liver.
Material & Methods: In this study, 24 male Wistar rats weighing 180±20 g were divided into four groups (positive control, negative control, first treatment, and second treatment). The two treatment groups received grapefruit extract 4 and 8 ml/kg by gavage for 5 weeks, respectively. Finally, the rats were anesthetized with chloroform, and after dissection, the liver tissue and blood samples were collected. Subsequently, RNA extraction and cDNA synthesis were performed using the relevant kits according to the protocol, and finally the expression of IL18 gene was performed using Real-time polymerase chain reaction (RT-PCR) technique, and the relevant analyzes were performed.
(Ethic code: IR.IAU.SHK.REC.1399.049)
Findings: In the present study, the results showed that grapefruit extract at a dose of 8 ml/kg could further reduce the expression of IL-18 gene, compared to a dose of 4 ml/kg in rats with fatty liver. This decrease in the rate expression was significant in the patient group (P<0.05). The results of biochemical tests confirmed liver improvement in treated rats at a dose of 8 ml/kg.
Discussion & Conclusion: Grapefruit juice seems to reduce various symptoms of inflammation due to its orange content by reducing the expression of the IL-18 gene in rats with a fatty liver that can be considered a therapeutic target in fatty liver disease.
Keywords: Fatty liver, Grapefruit, Interleukin 18 gene, Real-time (RT)-PCR technique
Full-Text [PDF 503 kb]   (252 Downloads)    
Type of Study: Research | Subject: biochemistry
Received: 2021/02/2 | Accepted: 2021/06/21 | Published: 2021/11/1
References
1. Lee BW, Lee YH, Park CY, Rhee EJ, Lee WY, Kim NH, et al. Non-alcoholic fatty liver disease in patients with type 2 diabetes mellitus a position statement of the fatty liver research group of the korean diabetes association. Diabetes MetabJ2020; 44:382-401. doi. 10.4093/dmj.2020.0010
2. Nolan CJ, Prentki M. Insulin resistance and insulin hypersecretion in the metabolic syndrome and type 2 diabetes time for a conceptual framework shift. Diabete Vascul Dis Res2019; 16:118-27. doi.10.1177/1479164119827611
3. Avan A, Tavakoly Sany SB, Ghayourmobarhan M, Rahimi HR, Tajfard M, Ferns G. Serum C‐reactive protein in the prediction of cardiovascular diseases overview of the latest clinical studies and public health practice. J Cell Physiol2018; 233:8508-25. doi.10.1002/jcp.26791
4. Kato K, Otsuka T, Saiki Y, Kobayashi N, Nakamura T, Kon Y, et al. Elevated c-reactive protein levels independently predict the development of prediabetes markers in subjects with normal glucose regulation. Exp Clin Endocrinol Diabete2019; 23:1-25. doi.10.1055/a-0869-7584
5. Williams CD, Stengel J, Asike MI, Torres DM, Shaw J, Contreras M, et al. Prevalence of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis among a largely middle aged population utilizing ultrasound and liver biopsy a prospective study. Gastroenterology 2011; 140:124-31. doi.10.1053/j.gastro.2010.09.038
6. Lankarani KB, Ghaffarpasand F, Mahmoodi M, Lotfi M, Zamiri N, Heydari ST, et al. Nonalcoholic fatty liver disease in southern Iran a population based study. Hepat Monthl 2013; 13:32-7. doi.10.5812/hepatmon.9248
7. Godos J, Federico A, Dallio M, Scazzina F. Mediterranean diet and nonalcoholic fatty liver disease: Molecular mechanisms of protection. Int J Food Sci Nut2017; 68:18-27. doi. 10.1080/09637486.2016.1214239
8. Hsu S. Modified green tea polyphenols and methods thereof for treating liver disease. Patents2015;1-20. doi.10/US20150056194A1/en
9. Razavi BM, Hosseinzadeh H. A Review of the effects of citrus paradisi and Its flavonoids, naringin, and naringenin in metabolic syndrome. Bio Food Dietar Int Diabet2019:515-43. doi.10.1016/B978-0-12-813822-9.00034-5
10. Seleim M, Hassan MA, Saleh A. Physico chemical evaluation of white and pink grapefruit Citrus paradisi juice. Assi J Agri Sci 2019; 50:112-22. doi. 10.21608/ajas.2019.52742
11. Wang W, Wu X, Chantapakul T, Wang D, Zhang S, Ma X, et al. Acoustic cavitation assisted extraction of pectin from waste grapefruit peels a green two stage approach and its general mechanism. Food Res Int 2017; 102:101-10. doi.10.1016/j.foodres.2017.09.087
12. Zhang NP, Liu XJ, Xie L, Shen XZ, Wu J. Impaired mitophagy triggers NLRP3 inflammasome activation during the progression from nonalcoholic fatty liver to nonalcoholic steatohepatitis. Lab Inv 2019; 99:749-63. doi.10.1038/s41374-018-0177-6
13. Elmahdy MK, Helal MG, Ebrahim TM. Potential anti-inflammatory effect of dapagliflozin in HCHF diet induced fatty liver degeneration through inhibition of TNFα and IL1β and interleukin 18 in Rat liver. Int Immunopharmacol 2020; 86:106730. doi.10.1016/j.intimp.2020.106730
14. Chen WY, Chen CJ, Liu CH, Mao FC. Chromium attenuates high fat diet induced nonalcoholic fatty liver disease in Mice. Biochem Biophys Res Commun2010; 397:459-64. doi.10.1016/j.bbrc.2010.05.129
15. Li Y, Li Li Z, Qin L, Ying W. Plasma interleukin 18 binding protein ratio in Chinese with NAFLD. Hepatogastroenterology2010; 57:103-6. doi.10/article/med/20422882
16. Chudnovskiy R, Thompson A, Tharp K, Hellerstein M, Napoli JL, Stahl A. Consumption of clarified grapefruit juice ameliorates high fat diet induced insulin resistance and weight gain in Mice. PLos One 2014; 9:108408. doi.10.1371/journal.pone.0108408
17. Lown KS, Bailey DG, Fontana RJ, Janardan SK, Adair CH, Fortlage LA, et al. Grapefruit juice increases felodipine oral availability in humans by decreasing intestinal CYP3A protein expression. J Clin Inv 1997; 99:2545-53.
18. Habibian M, Farzanegi P, Khakpour M. [The effect of circuit resistance training and medicago sativa supplement on interlukin18 and interlukin10 in young girls with low body mass index]. Qom Uni Med Sci J2015; 9:60-8. (Persian).
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: IR.IAU.SHK.REC.1399.049



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bahmanpoor S, Zia-Jahromi N. Evaluation of Grapefruit Juice in terms of Interleukin 18 Gene Expression in Rats with Fatty Liver and Healthy Rats. Journal of Ilam University of Medical Sciences 2021; 29 (4) :74-82
URL: http://sjimu.medilam.ac.ir/article-1-6986-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 29, Issue 4 (11-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.15 seconds with 31 queries by YEKTAWEB 4541