[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Editorial Board
Aims& Scopes
Journal News
Articles archive::
All Issues
Current Issue
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
Authors Guideline
Call for Papers
Submission Instruction
Review Process
Research Ethics Statements
Ethical issues
Author Complaint Process
For Reviewers::
Reviewers Section
Subscription::
Registration Information
Subscription Form
Contact us::
Contact Information
Contact us
Site Facilities::
Site map
Search contents
FAQ
Top 10 contents
Inform to friends
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations68044041
h-index2721
i10-index20497
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE
..
:: Volume 29, Issue 4 (10-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(4): 83-92 Back to browse issues page
Effect of Exercise and Kelussia Odaratissma Mozaff on the Expression of Atrogin-1 Gene in Cardiac Tissue of Obese Rats
Raziye Mahmoodi1 , Hossein Sazegar * 2
1- Dept of Biology, Faculty of Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Dept of Biology, Faculty of Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , hoseinsazgar@yahoo.com
Abstract:   (1733 Views)
Introduction: The Atrogin-1 gene is selectively expressed only in muscle tissue. Considering the importance of the Kelussia and Atrogin-1 gene, this study aimed to investigate the effect of exercise and Kelussia on the expression of Atrogin-1 gene in the heart tissue of obese rats.
Material & Methods: In total, 30 adult male Wistar rats weighing 180 to 200 g were selected and divided into five groups of control, negative control (obese mice), two treatment groups receiving doses of 400 and 800 mg/kg of Kelussia extract, and a group of obese mice along with exercise. It should be noted that a rat treadmill was used in which the rats were placed on the device five days a week for one hour. Atrogin-1 gene expression was assessed using Real-time (RT)-PCR technique, and finally, the results were analyzed using SPSS software through an independent t-test. A p-value less than 0.05 was considered statistically significant.
(Ethic code: IR.IAU.SHK.REC.1399.050)
Findings: The results showed that Kelussia extract at a dose of 800 mg/kg could significantly reduce the expression of Atrogin-1 gene (1.15±0.33b), compared to 400 (1.27±0.50ab) and the obese group. On the other hand, the group of mice with exercise also had a significant decrease in Atrogin-1 gene expression, compared to the obese group (1.75±0.84ab). The results of biochemical tests also confirmed the improvement of the heart of obese mice with 800 mg/kg.
Discussion & Conclusion: Kelussia due to compounds, such as flavonoids, can reduce the expression of Atrogin-1 gene. If it is confirmed in future studies, it can be a therapeutic target in the hearts of obese people.
Keywords: Atrogin-1 gene, Kelussia, Heart tissue, Obesity
Full-Text [PDF 592 kb]   (810 Downloads)    
Type of Study: Research | Subject: biochemistry
Received: 2021/02/2 | Accepted: 2021/06/21 | Published: 2021/11/1
References
1. Eckel RH, Krauss RM. American Heart Association call to action: obesity as a major risk factor for coronary heart disease. Circulation 1998;97: 2099-100. doi.10.1161/01.CIR.97.21.2099
2. Allender S, Scarborough P, Peto V, Rayner M, Leal J, Luengofernandez R, et al. European cardiovascular disease statistics. European Heart Network2008;3: 11-35.
3. Samiee RF, Ziaee A, Qambarian A, Mirmiran P, Momenan A, Azizi F. [Association between risk factors of cardiovascular diseases and obesity among Tehranian Women]. TLGSJ2012;2:1-20. (Persian)
4. Pilote L, Dasgupta K, Guru V, Humphries KH, Mcgrath J, Norris C, et al. A comprehensive view of sex-specific issues related to cardiovascular disease. CMAJ2007;176: 1-44. doi.10.1503/cmaj.051455
5. Perl A. Mtor activation is a biomarker and a central pathway to autoimmune disorders, cancer, obesity, and aging. Ann Newyork Acad Sci 2015;1346: 33. doi.10.1111/nyas.12756
6. Milan G, Romanello V, Pescatore F, Armani A, Paik JH, Frasson L, et al. Regulation of autophagy and the ubiquitin proteasome system by the foxo transcriptional network during muscle atrophy. Nature Com2015;6: 6670. doi.10.1038/ncomms7670
7. Martins R, Lithgow GJ, Link W. Long live foxo unraveling the role of foxo proteins in aging and longevity. Ag Cell 2016;15: 196-207. doi. 10.1111/acel.12427
8. Bodine SC, Latres E, Baumhueter S, Lai VKM, Nunez L, Clarke BA, et al. Identification of ubiquitin ligases required for skeletal muscle. Atroph Sci 2001;294: 1704-8. doi. 10.1126/science.1065874
9. Tsi D, Das N, Tan B. Effects of aqueous celery Apium graveolens extract on lipid parameters of rats fed a high fat diet. Plant Med1995;61: 18-21. doi.10.1055/s-2006-957990
10. Kaouadjii M. Nouveaa glycoside befphtalid chezgentiona pedicellata. J Nat Prod. 1986;49: 872-87.
11. Ferreira AC, Neto JC, Silva AC, Kuster RM, Carvalho DP. Inhibition of thyroid peroxidase by Myrcia uniflora flavonoids. Chem Res Toxicol 2006;19: 351-5. doi.org/10.1021/tx0501684
12. Rychlik W. Oligo 7 primer analysis software PCR Primer design. 2 th ed. Springer Publication. 2007;P. 35-59. doi.10.1007/978-1-59745-528-2_2
13. Rahimi Z, Salehi M, Dousti A. [CCL2 polymorphism in drug resistant and drug-responsive patients with epilepsy in Isfahan Iran]. Med Lab J 2017;11: 30-4. (Persian)
14. Jin B, Li YP. Curcumin prevents lipopolysaccharide induced atrogin 1/MAFbx upregulation and muscle mass loss. J Cell Biochem2007;100: 960-9. doi.10.1002/jcb.21060
15. Ramezani M, Nasri S, Yassa N. Study of anti inflammatory effect of aqueous and hexane extract of Apium graveolens L. in Mice.Iranian J Med Arom Plants 2009;24: 437-43. doi.cabdirect/abstract/20093145047
16. Zanchi NE, Siqueirafilho MA, Lira FS, Rosa JC, Yamashita AS, Oliveira CR, et al. Chronic resistance training decreases MuRF1 and Atrogin1 gene expression but does not modify Ak and GSK-3β and p70S6K levels in Rats. European J Appl Physiol 2009;106: 415-23. doi.10.1007/s00421-009-1033-6
17. Dang K, Li YZ, Gong LC, Xue W, Wang HP, Goswami N, et al. Stable atrogin1 and murf1 gene expression is involved in the protective mechanism in soleus muscle of hibernating daurian ground squirrels Spermophilus dauricus. Biology Open 2016;5: 62-71. doi. 10.1242/bio.015776
18. Kafeshani M, Mirhosseini M, Momeni A, Rabiei R, Rafieiankopaei M. Impact of Kelussia odoratissima mozaffarian lipid profile and fasting blood sugar in hyperlipidemia patients. J Nephropharmacol2017;6: 9.
19. Hoseini A. [The effect of eight weeks of curcumin supplementation on the expression of Some regulatory genes of atrophic processes in the heart tissue of fatty adult fatty Rats]. J Fasa Uni Med Sci 2019;9: 1425-32. (Persian).
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: IR.IAU.SHK.REC.1399.050

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mahmoodi R, Sazegar H. Effect of Exercise and Kelussia Odaratissma Mozaff on the Expression of Atrogin-1 Gene in Cardiac Tissue of Obese Rats. J. Ilam Uni. Med. Sci. 2021; 29 (4) :83-92
URL: http://sjimu.medilam.ac.ir/article-1-6985-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 29, Issue 4 (10-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.15 seconds with 41 queries by YEKTAWEB 4666