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:: Volume 29, Issue 5 (11-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(5): 12-21 Back to browse issues page
Expression of Apoptotic Genes after Autotransplantation of Vitrified Rat Ovary Encapsulated with Hyaluronic Acid Hydrogel
Maryam Akhavan Taheri1 , Mojtaba Rezazadeh valojerdi * 2, Bita Ebrahimi3
1- Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran
2- Dept of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , mr_valojerdi@modares.ac.ir
3- Dept of Embryology, Reproductive Biomedicine Institute, Royan Institute, Tehran, Iran
Abstract:   (1094 Views)
Introduction: Ischemia followed by apoptosis and follicles mortality are problems that occur after cryopreservation and ovarian tissue transplantation. The present study used hyaluronic acid hydrogel as a capsule to reduce ischemia and apoptosis in vitrified ovarian tissue transplantation of rats.
Material & Methods: In total, 22 adult female rats (~ 8-week old) with normal estrous cycle were ovariectomized, and their right ovaries were then vitrified and divided into two groups after being warmed, including vitrified-transplanted (VT) (n=11) and vitrified-encapsulated in hyaluronic acid hydrogel-transplanted (VT+HA) (n=11) that were auto transplanted into the dorsal muscle. Following that, a daily vaginal smear was obtained from the 4th day after transplantation of the rats. The ovaries were removed at the end of the first estrous cycle (approximately 15 days after transplantation), and some apoptotic genes including P53, c-Myc, Bax, Bcl-2, and Caspase 3 were evaluated by the real-time PCR.
Findings: All transplants were completely successful (100%). The results also showed that the expression of the P53, c-Myc, Bax, and Caspase 3 genes were higher in the VT group, compared to the VT+HA group. However, this difference was statistically significant only in the c-Myc gene (P<0.05).
Discussion & Conclusion: Hyaluronic acid hydrogel was able to reduce the rate of apoptosis in the capsule group (VT+HA), compared to the non-capsule group (VT) after transplantation.
Keywords: Apoptosis, Hyaluronic acid hydrogel, Ovarian tissue, Ovarian transplantation, Vitrification
Full-Text [PDF 537 kb]   (303 Downloads)    
Type of Study: Research | Subject: biology
Received: 2020/11/26 | Accepted: 2021/07/18 | Published: 2022/01/5
1. Demeestere I, Simon P, Emiliani S, Delbaere A, Englert Y. Orthotopic and heterotopic ovarian tissue transplantation. Hum Rep Up2009;15:649-65. doi.10.1093/humupd/dmp021.
2. Marin L, Bedoschi G, Kawahara T, Oktay KH. History evolution and current state of ovarian tissue autotransplantation with cryopreserved tissue a successful translational research Journey from 1999 to 2020. Rep Sci 2020:1-8. doi.10.1007/s43032-019-00066-9.
3. Kim S, Lee Y, Lee S, Kim T. Ovarian tissue cryopreservation and transplantation in patients with cancer. Obste Gynecol Sci 2018;61:431. doi. 10.5468/ogs.2018.61.4.431.
4. Donnez J, Dolmans M, Demylle D, Jadoul P, Pirard C, Squifflet J, et al. Restoration of ovarian function after orthotopic intraovarian and periovarian transplantation of cryopreserved ovarian tissue in a woman treated by bone marrow transplantation for sickle cell anaemia case report. Hum Rep 2006;21:183-8. doi.10.1093/ humrep/dei268
5. Santos R, Amorim C, Cecconi S, Fassbender M, Imhof M, Lornage J, et al. Cryopreservation of ovarian tissue: an emerging technology for female germline preservation of endangered species and breeds. Anim Rep Sci2010;122:151-63. doi.10.1016/j.anireprosci.2010.08.010
6. Oktay K. Ovarian tissue cryopreservation and transplantation preliminary findings and implications for cancer patients. Hum Rep Up 2001;7:526-34 . doi.10.1093/humupd/7.6.526
7. Fathi R, Rezazadehvalojerdi M, Salehnia M, Ebrahimi B, Salmanyazdi R. Ovarian tissue transplantation advantages disadvantages and upcoming challenges. J Mazandaran Uni Med Sci 2014;24:253-65
8. Amorim CA, Curaba M, Van Langendonckt A, Dolmans MM, Donnez J. Vitrification as an alternative means of cryopreserving ovarian tissue. Rep Biomed 2011;23:160-86
9. Hossay C, Donnez J, Dolmans MM. Whole ovary cryopreservation and transplantation: a systematic review of challenges and research developments in animal experiments and humans. J Clin Med 2020;9:3196. doi.10.3390/jcm9103196
10. Roness H, Meirow D. Fertility preservation follicle reserve loss in ovarian tissue transplantation. Reproduction 2019;158:35-44. doi.10.1530/REP-19-0097.
11. Damous LL, Silva SMd, Lopes RAM, Sakano CRdSB, Simoes MdJ, Montero EFDS. Study on the vaginal smear of rats submitted to autologous ovarian transplant impact of remote ischemic preconditioning. Acta Cir Brasile2009;24:387-92. doi.10.1590/S0102-86502009000500009
12. Gao JM, Yan J, Li R, Li M, Yan LY, Wang TR, et al. Improvement in the quality of heterotopic allotransplanted mouse ovarian tissues with basic fibro Liu L, Wood G, Morikawa L, Ayearst R, Fleming C, McKerlie C. Restoration of fertility by orthotopic transplantation of frozen adult Mouse ovaries. Hum Rep 2008;23:122-8. doi.10.1093/humrep/dem348
13. Shiraztehrani A, Mazoochi T, Akhavantaheri M, Salehnia M. The effects of ovarian encapsulation with alginate hydrogel on morphology and follicular count of vitrified Mouse ovary. J Feyz2019;23:467-75
14. Tehrani AS, Mazoochi T, Taheri MA, Aghadavood E, Salehnia M. The effects of ovarian encapsulation on morphology and expression of apoptosis related genes in vitrified Mouse ovary. J Rep Inf2021;22:23 . doi.10.18502/jri.v22i1.4992
15. Gao J, Huang Y, Li M, Zhao H, Zhao Y, Li R, et al. Effect of local basic fibroblast growth factor and vascular endothelial growth factor on subcutaneously allotransplanted ovarian tissue in ovariectomized Mice. PLos One 2015;10: 134035. doi.10.1371/journal.pone.0134035
16. Fraser J, Laurent T, Laurent U. Hyaluronan: its nature, distribution, functions and turnover. J Int Med 1997;242:27-33. doi.10.1046/j.1365-2796.1997.00170.x
17. Sattar A, Rooney P, Kumar S, Pye D, West DC, Scott I, et al. Application of angiogenic oligosaccharides of hyaluronan increases blood vessel numbers in rat skin. J Invest Dermatol1994;103:576-9. doi.10.1111/1523-1747. ep12396880.
18. Morita Y, Tilly JL. Oocyte apoptosis: like sand through an hourglass. Dev Biol 1999;213:1-17. doi.10.1006/dbio.1999.9344.
19. Kagawa N, Silber S, Kuwayama M. Successful vitrification of bovine and human ovarian tissue. Rep Biomed 2009;18:568-77. doi.10.1016/s1472-6483(10)60136-8.
20. Akhavan Taheri M, Rezazadeh Valojerdi M, Ebrahimi B. Intramuscular autotransplantation of vitrified rat ovary encapsulated with hyaluronic acid hydrogel. Biopres Biobank2016;14:114-21. doi.10.1089/bio.2015.0021
21. Wang L, Ying YF, Ouyang YL, Wang JF, Xu J. VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental Rabbit model. J Assist Rep Genet 2013;30:1301-11. doi.10.1007/s10815-013-0043-9
22. Tavana S, Azarnia M, Valojerdi MR, Shahverdi A. Hyaluronic acid based hydrogel scaffold without angiogenic growth factors enhances ovarian tissue function after autotransplantation in Rats. Biomedical Mate2016;11: 55006. doi.10.1088/6041/11/5/055006
23. Höpker K, Hagmann H, Khurshid S, Chen S, Schermer B, Benzing T, et al. Putting the brakes on p53 driven apoptosis. Cell Cycle2012;11:4122-8. doi.10.4161/cc.21997
24. Prendergast GC. Mechanisms of apoptosis by c Myc. Oncogene1999;18:2967-87. doi.10.1038/ sj.onc.1202727
25. Evan GI, Wyllie AH, Gilbert CS, Littlewood TD, Land H, Brooks M, et al. Induction of apoptosis in fibroblasts by c myc protein. Cell1992;69:119-28. doi.10.1016/0092-8674(92)90123-T
26. Dlamini Z, Mbita Z, Zungu M. Genealogy, expression, and molecular mechanisms in apoptosis. Pharmacol Ther2004;101:1-15. d doi.10.1016/j.pharmthera.2003.08.005
27. Schwerk C, Schulze-Osthoff K. Non-apoptotic functions of caspases in cellular proliferation and differentiation. Biochem Pharmacol. 2003;66(8):doi: 10.1016/S0006-2952(03)00497-0
28. Köhler C, Orrenius S, Zhivotovsky B. Evaluation of caspase activity in apoptotic cells. J Immunol Meth2002;265:97-110. doi.10.1016/S0022-1759(02)00073-X
29. Tavana S, Valojerdi MR, Azarnia M, Shahverdi A. Restoration of ovarian tissue function and estrous cycle in rat after autotransplantation using hyaluronic acid hydrogel scaffold containing VEGF and bFGF. Growth Fact 2016;34:97-106. doi.10.1080/08977194.2016.1194835
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Akhavan Taheri M, Rezazadeh valojerdi M, Ebrahimi B. Expression of Apoptotic Genes after Autotransplantation of Vitrified Rat Ovary Encapsulated with Hyaluronic Acid Hydrogel. J. Ilam Uni. Med. Sci. 2021; 29 (5) :12-21
URL: http://sjimu.medilam.ac.ir/article-1-6875-en.html

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Volume 29, Issue 5 (11-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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