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:: Volume 28, Issue 4 (10-2020) ::
Journal of Ilam University of Medical Sciences 2020, 28(4): 1-11 Back to browse issues page
Effects of Concurrent Training (Resistance-Endurance) on Interferon-γ, Interloukin-18 and Transforming Growth Factor-β Gene Expression in Women with Type 2 Diabetes
Amirabbas Monazzami * 1
1- Dept of Sport Physiology, Faculty of Sports Sciences, Razi University, Kermanshah, Iran , monazzami.amirabbas@gmail.com
Abstract:   (1899 Views)
Introduction: High-intensity training increases the expression of anti-inflammatory cytokines and decreases the expression of pre-inflammatory cytokines in healthy individuals. However, there is limited research investigating the role of concurrent training in the expression of these cytokines in diabetics. This study aimed to evaluate the effect of concurrent training on the gene expression of pre-inflammatory and anti-inflammatory cytokines in females with type 2 diabetes.
Materials & Methods: In total, 12 diabetic patients (age range: 30-38 years) were selected randomly and divided into concurrent training (healthy cases, n=6), concurrent training (diabetic cases, n=6), and control (diabetic cases, n=6). The concurrent training protocol consisted of three isometric resistance training sessions with 3 sets of 8 with 80% of their one maximum repetition (  per week. Moreover, the endurance training included 30-min running (3 sets of 10 min) on a treadmill with a 70%-80% maximum heart rate. The amount of the mRNA gene expression in leukocyte (Interferon-γ[IFN-γ], Interloukin-18[IL-18], and Transforming Growth Factor [TGF-β]) was determined by the Real time-PCR technique. Quantitative gene expression was calculated using the 2-ΔΔ CT method. The differences in variables were determined by the independent t-test (permutation test) and one-way ANOVA. Ethics code: 1393.10.15
Findings: The healthy and diabetic cases showed no significant difference in terms of reduction in the IFN-γ and TGF-β mRNA gene expression after training, compared to the control group (P>0/05). In addition, the results showed that IL-18 mRNA gene was not expressed in leukocytes of healthy and diabetic cases immediately after training (P>0.05).
Discussions & Conclusions: In conclusion, the results suggest that concurrent training in one session is not able to create an alteration in the expression of IFN-γ, IL-18, and TGF-β. This type of exercise training may require a longer duration and greater intensity to make changes.
Keywords: Type 2 Diabetes, Concurrent Training, TGF-β, IFN-γ, IL-18
Full-Text [PDF 1031 kb]   (728 Downloads)    
Type of Study: Research | Subject: Molecular Genetics
Received: 2020/05/19 | Accepted: 2020/09/16 | Published: 2020/10/31
1. Gokhale R, Chandrashekara S, Vasanthakumar KC. Cytokine response to strenuous exercise in athletes and non athlete an adaptive response. Cytokine2007;40:123-7. doi: 10.1016/j.cyto.2007.08.006.
2. Medeiros NDS, Abreu FG, Colato AS, Lemos LS, Ramis TR, Dorneles GP, et al. Effects of concurrent training on oxidative stress and insulin resistance in obese individuals. Oxida Med Cell Longe2015; 697181. doi.10.1155/2015/697181.
3. Kuryłowicz A, Kozniewski K. Anti inflammatory strategies targeting metaflammation in type 2 diabetes . Molecules 2020; 25: 2224. doi.10.3390/molecules25092224.
4. Correa, Fernanda O B. The short-term effectiveness of non-surgical treatment in reducing levels of interleukin-1beta and proteases in gingival crevicular fluid from patients with type 2 diabetes mellitus and chronic periodontitis. J Periodontol 2008;11: 2143-50. doi: 10.1902/jop.2008.080132.
5. Carraro L, Ferraresso S, Cardazzo B, Romualdi C, Montesissa C, Gottardo F, et al. Expression profiling of skeletal muscle in young bulls treated with steroidal growth promoters. Physiol Genom2009; 38.138-48. doi.10.1152/physiol gen.00014.
6. Lee YH, Bae SC. Association between interferon-γ +874 T/A polymorphism and susceptibility to autoimmune diseases a meta-analysis. Lupus 2016; 25:710-718. doi. 10.1177/0961203315624557.
7. Cruz M, Maldonado C, Mondragon R, Sanchez R, Wacher NH, Carvajal G, et al. Glycine treatment decreases proinflammatory cytokines and increases interferon gamma in patients with type 2diabetes. J Endo Invest2008;31:.694-9. doi.10.1007/BF03346417.
8. mcgillicuddy FC, Chiquoine EH, Hinkle CC, Kim RJ, Shah R, Roche HM, et al. Interferon gamma attenuates insulin signaling, lipid storage and differentiation in human adipocytes via activation of the JAK/STAT pathway. J Biol Chem 2009;284:31936-44. doi.10.1074/jbc. M109.061655
9. Katarzyna S, Rafal M, Anna ZM, Anna PK, Piotr S, Natalia WK, et al. Interleukin6 and Interleukin15 as possible biomarkers of the risk of autoimmune diabetes development. Biomed Res Int2019; 4734063. doi.10.1016/j. biopsycho. 2014.03.009
10. Leick L, Lindegaard B, Stensvold D, Plomgaard P, Saltin B, Pilegaard H. Adipose tissue interleukin18 mRNA and plasma interleukin18 effect of obesity and exercise. Obesit Silve Spring 2007;15:356-63. doi.10.1038/oby.2007.528
11. Colato A, Abreu F, Medeiros N, Lemos L, Dorneles G, Ramis T, et al. Effects of concurrent training on inflammatory markers and expression of CD4 and CD8 and HLA-DR in overweight and obese adults. J Exe Sci Fit2014; 12:55-61. doi.10.1016/j.jesf.2014.06.002
12. Bastard JP, Maachi M, Lagathu C, Kim MJ, Caron M, Vidal H, et al. Recent advances in the relationship between obesity inflammation, and insulin resistance. European Cyt Net2006; 17:4-12.
13. Saremi A. [Comparison of the effects of endurance resistance and concurrent training on insulin resistance and adiponectin-leptin ratio in diabetic Rat]. J QUMS2017; 21:13-22. (Persian)
14. Emma LL, Andrew HB, Angela CB. TGFβ smooth muscle cells and coronary artery disease a review. Cel Sig 2019; 53: 90-101. doi.10.1016/j.cellsig.2018.09.004
15. Zanuso S, Jimenez A, Pugliese G, Corigliano G, Balducci S. Exercise for the management of type 2 diabetes a review of the evidence. Acta Diabetol 2010;47:15-22. doi. 0.1007/s00592-009-0126-3
16. Thomas W, Buford MBC, Brian D, Shelmadine M. Effects of eccentric treadmill exercise on inflammatory gene expression in human skeletal muscle. Appl Physiol Nutr Met. 2009;34:745-53. doi.org/10.1139/H09-067.
17. Cheyne E, Donges RD, Greg C, Michael J. Cytokine mRNA expression responses to resistance aerobic and concurrent exercise in sedentary middle aged men. Appli Physiol Nut Metab2014;39:130-7. doi.10. 1139/apnm-2013076
18. Monazzamiamirabbas RH, Ghrakhanlou R, Yari Kh, Rahimi Z. Modulation of oxidative and glycolytic skeletal muscle fibers Na+/H+ exchanger1 and Na+/HCO3- co-transporter genes and proteins expression in type2 diabetic Rat streptozotocin + high fat diet following long term endurance training. CMB2017;63:8-11. doi.10.14715/cmb/2017.63.5.3
19. Libardi CA, Souza GV, Cavaglieri CR, Madruga VA, et al. Effect of resistance endurance and concurrent training on TNF-α, IL-6 and CRP. Med Sci Spor Exe 2012; 44; 51-6. doi. 10.1249/MSS.0b013e318229d2e9
20. Atashak S, Stannard SR, Azizbeigi k. Cardiovascular risk factors adaptation to concurrent training in overweight sedentary middle aged men. J Sport Med Phys Fit2016; 56:624-30
21. Yamamoto SN, Umeda T, Matsuzaka M, Takahashi I, Tanabe M, Danjo K, Kojima A, et al. Effects of long term training on neutrophil function in male university judoists. British J Sport Med2008;42:255-9. doi.10.1074/jbc.RA119.009596
22. Tammela T, Enholm B, Alitalo K, Paavonen K. The biology of vascular endothelial growth factors. Cardio Res2005;65:550-63. doi.10.1016/j.cardiores.2004.12.002
23. Atashak S. [The effect of the eight-week progressive concurrent training on inflammatory index of cardiovascular disease predictor, and body composition in sedentary middle age Men]. Iranian J Cardiovas Nurs2013; 2:16-25. (Persian)
24. Eskandary S, Rahimi E. [Effects of eight weeks aerobic training, resistance training and concurrent training on the metabolic syndrome and HbA1c in men with type 2 diabetes]. J Phys Act Horm2017; 1; 51-64. (Persian).
25. Aikens JE, Perkins DW, Piette JD, Lipton B. Association between depression and concurrent Type 2 diabetes outcomes varies by diabetes regimen. Diabet Med2008; 25:1324-9. doi. 10.1111/j.1464-5491
26. Abdi A, Mehrabani J, Nordvall M, Wong A, Fallah A, Bagheri R. Effects of concurrent training on irisin and fibronectin type III domain containing 5 expression in visceral adipose tissue in type-2 diabetic rats. Arch Physiol Biochem2020;1-6. doi.10.1080/13813455.2020.1716018
27. Ogorman DJ, Karlsson HK, Mcquaid S, Yousif O, Rahman Y, Gasparro D, et al. Exercise training increases insulin stimulated glucose disposal and GLUT4 protein content in patients with type 2 diabetes. Diabetologia 2006;49:2983-92. doi.10.1007/s00125-006-457-3
28. Balducci S, Zanuso S, Nicolucci A, Fernando F, Cavallo S, Cardelli P, et al. Anti inflammatory effect of exercise training in subjects with type 2 diabetes and the metabolic syndrome is dependent on exercise modalities and independent of weight loss. Nut Metab Cardiovas Dis2010;20:608-17. doi.10.1016/j.numecd.2009.04.015
29. Bruun JM, Stallknecht B, Helge JW, Richelsen B. Interleukin18 in plasma and adipose tissue effects of obesity, insulin resistance and weight loss. European J Endocrinol2007;157:465-71. doi.10.1530/ EJE-07-0206.
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monazzami A. Effects of Concurrent Training (Resistance-Endurance) on Interferon-γ, Interloukin-18 and Transforming Growth Factor-β Gene Expression in Women with Type 2 Diabetes. Journal of Ilam University of Medical Sciences 2020; 28 (4) :1-11
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Volume 28, Issue 4 (10-2020) Back to browse issues page
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