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:: Volume 30, Issue 1 (3-2022) ::
Journal of Ilam University of Medical Sciences 2022, 30(1): 1-10 Back to browse issues page
Investigation of the Effect of Polycystic Ovary Syndrome on the Expression of the Sirtuin-3 Gene in the Ovum of the NMRI Mice
Hanieh Jalali * 1, Mahnaz Azarnia2 , Fatemeh Kamalipour2
1- Dept of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran , jalali@khu.ac.ir
2- Dept of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Abstract:   (2261 Views)
Introduction: Increasing levels of free radicals in the ova reduce the quality of their fertilization. Investigations have revealed the elevated production of tissue and serum-free radicals in women with polycystic ovary syndrome. Sirtuin-3 is located in mitochondria and plays an important role in the deactivation of free radicals. This study aimed to investigate the expression of the Sirtuin-3 gene in the ova of polycystic ovary syndrome affected mice.
Material & Methods: Polycystic ovary syndrome was induced by the injection of estradiol valerate (40 mg/kg) in six NMRI mice (age: six weeks old; weight: 25±5); moreover, six mice were selected as a control group. Following the induction, the structure of the ovaries was examined using the histotechnique, and the serum levels of sex hormones were determined using ELISA. The ova were collected from the ovaries, and their RNA was extracted by a special kit and converted to cDNA. Real-Time PCR was performed to analyze the expression of Sirtuin-3, and the Gapdh gene was selected as an internal control.
(Ethic code: 97/6505)
Findings: In the mice with polycystic ovary syndrome, hydatid cysts were formed in the ovaries. Furthermore, serum FSH hormone levels decreased in the affected mice, while LH and testosterone levels elevated, compared to those in the control mice (P<0.001). The expression of Sirtuin-3 was reduced in the ova of mice with polycystic ovary syndrome, compared to that in healthy mice in the ratio of 0.18 (P<0.05).
Discussion & Conclusion: The result of the present study showed that the expression of the Sirtuin-3 gene was decreased in the ovum of mice with polycystic ovary syndrome. Moreover, the disrupted expression of the Sirtuin-3 in the ovum can be suggested as one of the causes of reduced quality of the ovum in patients with polycystic ovary syndrome.
Keywords: Mitochondria, Ovum, Oxidative stress, Polycystic ovarian syndrome
Full-Text [PDF 610 kb]   (936 Downloads)    
Type of Study: Research | Subject: biology
Received: 2020/05/9 | Accepted: 2021/12/21 | Published: 2023/03/6
1. Conti M, Franciosi F. Acquisition of oocyte competence to develop as an embryo: integrated nuclear and cytoplasmic events. Hum Reprod Update. 2018;24:3:245-66. doi: org/10.1093/humupd/dmx040
2. Reader KL, Stanton JL, Juengel JL. The Role of Oocyte Organelles in Determining Developmental Competence. Biology (Basel). 2017; 6: 1-3. doi:10.3390/biology6030035
3. Swain JE, Pool TB. ART failure: oocyte contributions to unsuccessful fertilization. Hum Reprod Update. 2008;14:5:431-46. doi: 10.1093/humupd/dmn025
4. Chappel S. The role of mitochondria from mature oocyte to viable blastocyst. Obstet Gynecol Int. 2013;2013:183024. doi:org/10.1155/2013/183024
5. Bentov Y, Yavorska T, Esfandiari N, Jurisicova A, Casper RF. The contribution of mitochondrial function to reproductive aging. J Assist Reprod Genet. 2011;28:9:773-83. doi: 10.1007/s10815-011-9588-7
6. Lord T, Aitken RJ. Oxidative stress and ageing of the post-ovulatory oocyte. Reproduction. 2013;146:6:R217-27. doi: 10.1530/REP-13-0111
7. Cheeseman KH, Slater TF. An introduction to free radical biochemistry. Br Med Bull. 1993;49:3:481-93. doi: 10.1093/oxfordjournals.bmb.a072625
8. McCord JM. The evolution of free radicals and oxidative stress. Am J Med. 2000; 108:652-9. doi: 10.1016/s0002-9343(00)00412-59.
9. Nakagawa T, Guarente L. Sirtuins at a glance. J Cell Sci. 2011;124:833-8. doi: 10.1242/jcs.081067
10. Bause AS, Haigis MC. SIRT3 regulation of mitochondrial oxidative stress. Exp Gerontol. 2013;48:7:634-9 doi: 10.1016/j.exger.2012.08.007.
11. Tseng AH, Shieh SS, Wang DL. SIRT3 deacetylates FOXO3 to protect mitochondria against oxidative damage. Free Radic Biol Med. 2013;63:222-34. doi: 10.1016/j.freeradbiomed.2013.05.002
12. Kim HS, Patel K, Muldoon-Jacobs K, Bisht KS, Aykin-Burns N, Pennington JD, et al. SIRT3 is a mitochondria-localized tumor suppressor required for maintenance of mitochondrial integrity and metabolism during stress. Cancer Cell. 2010;17:1:41-52. doi:10.1016/j.ccr.2009.11.023
13. Witchel SF, Oberfield SE, Pena AS. Polycystic Ovary Syndrome: Pathophysiology, Presentation, and Treatment With Emphasis on Adolescent Girls. J Endocr Soc. 2019. 2019; 14:3:1545-1573. doi: 10.1210/js.2019-00078
14. Azziz R, Carmina E, Chen Z, Dunaif A, Laven JS, Legro RS, et al. Polycystic ovary syndrome. Nat Rev Dis Primers. 2016;2:16057. doi: 10.1038/nrdp.2016.57
15. Murri M, Luque-Ramirez M, Insenser M, Ojeda-Ojeda M, Escobar-Morreale HF. Circulating markers of oxidative stress and polycystic ovary syndrome (PCOS): a systematic review and meta-analysis. Hum Reprod Update. 2013;19:3:268-88. doi: 10.1093/humupd/dms059
16. Zuo T, Zhu M, Xu W. Roles of Oxidative Stress in Polycystic Ovary Syndrome and Cancers. Oxid Med Cell Longev. 2016;2016:8589318. doi: 10.1155/2016/8589318
17. Sasaki H, Hamatani T, Kamijo S, Iwai M, Kobanawa M, Ogawa S, et al. Impact of Oxidative Stress on Age-Associated Decline in Oocyte Developmental Competence. Front Endocrinol (Lausanne). 2019;10:811. doi.org/10.3389/fendo.2019.00811
18. Das S, Chattopadhyay R, Ghosh S, Ghosh S, Goswami SK, Chakravarty BN, et al. Reactive oxygen species level in follicular fluid--embryo quality marker in IVF? Hum Reprod. 2006;21:9:2403-7. doi:10.1093/humrep/del156
19. Combelles CM, Gupta S, Agarwal A. Could oxidative stress influence the in-vitro maturation of oocytes? . Reprod Bio Online. 2009;18:6:864-80. doi: 10.1016/s1472-6483(10)60038-7
20. Osuka S, Nakanishi N, Murase T, Nakamura T, Goto M, Iwase A, et al. Animal models of polycystic ovary syndrome: A review of hormone-induced rodent models focused on hypothalamus-pituitary-ovary axis and neuropeptides. Reprod Med Biol. 2019;18:2:151-60. doi: 10.1002/rmb2.12262
21. Yanez LZ, Han J, Behr BB, Pera RAR, Camarillo DB. Human oocyte developmental potential is predicted by mechanical properties within hours after fertilization. Nat Commun. 2016; 7:1-12. doi: 10.1038/ncomms10809.
22. Liu Q, Li Y, Feng Y, Liu C, Ma J, Li Y, et al. Single-cell analysis of differences in transcriptomic profiles of oocytes and cumulus cells at GV, MI, MII stages from PCOS patients. Sci Rep. 2016; 6:1-12. doi: 10.1038/srep39638
23. Roth LW, McCallie B, Alvero R, Schoolcraft WB, Minjarez D, Katz-Jaffe MG. Altered microRNA and gene expression in the follicular fluid of women with polycystic ovary syndrome. J Assist Reprod Genet. 2014;31:3:355-62. doi: 10.1007/s10815-013-0161-4
24. Wood JR, Dumesic DA, Abbott DH, Strauss JF, 3rd. Molecular abnormalities in oocytes from women with polycystic ovary syndrome revealed by microarray analysis. J Clin Endocrinol Metab. 2007;92:2:705-13. doi: 10.1210/jc.2006-2123.
25. Fang L, Wang S, Li Y, Yu Y, Li Y, Yan Y, et al. High GDF-8 in follicular fluid is associated with a low pregnancy rate in IVF patients with PCOS. Reproduction. 2020; 160:11-19. doi: 10.1530/REP-20-0077
26. Zhao HC, Ding T, Ren Y, Li TJ, Li R, Fan Y, et al. Role of Sirt3 in mitochondrial biogenesis and developmental competence of human in vitro matured oocytes. Hum Reprod. 2016;31:3:607-22. doi: 10.1093/humrep/dev345
27. Kwak SS, Cheong SA, Yoon JD, Jeon Y, Hyun SH. Expression patterns of sirtuin genes in porcine preimplantation embryos and effects of sirtuin inhibitors on in vitro embryonic development after parthenogenetic activation and in vitro fertilization. Theriogenology. 2012;78:7:1597-610. doi: 10.1016/j.theriogenology.2012.07.006
28. Tatone C, Di Emidio G, Vitti M, Di Carlo M, Santini S, Jr., D'Alessandro AM, et al. Sirtuin Functions in Female Fertility: Possible Role in Oxidative Stress and Aging. Oxid Med Cell Longev. 2015; 2015:1-12. doi:org/10.1155/2015/659687
29. Kawamura Y, Uchijima Y, Horike N, Tonami K, Nishiyama K, Amano T, et al. Sirt3 protects in vitro-fertilized mouse preimplantation embryos against oxidative stress-induced p53-mediated developmental arrest. J Clin Invest. 2010;120:8:2817-28. doi: 10.1172/JCI42020
30. Han L, Wang H, Li L, Li X, Ge J, Reiter RJ, et al. Melatonin protects against maternal obesity-associated oxidative stress and meiotic defects in oocytes via the SIRT3-SOD2-dependent pathway. J Pineal Res. 2017; 63: 1-10. doi: 10.1111/jpi.12431
31. Liu X, Zhang L, Wang P, Li X, Qiu D, Li L, et al. Sirt3-dependent deacetylation of SOD2 plays a protective role against oxidative stress in oocytes from diabetic mice. Cell Cycle. 2017;16:13:1302-8. doi: 10.1080/15384101.2017.1320004
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Jalali H, Azarnia M, Kamalipour F. Investigation of the Effect of Polycystic Ovary Syndrome on the Expression of the Sirtuin-3 Gene in the Ovum of the NMRI Mice. J. Ilam Uni. Med. Sci. 2022; 30 (1) :1-10
URL: http://sjimu.medilam.ac.ir/article-1-6515-en.html

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Volume 30, Issue 1 (3-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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