The heavy metal pollution has become one of the most important environmental problems. Heavy metals are the most important environmental pollutants, especially for human health and aquatic systems are a serious threat. Iranian Research Organization for Science and Technology of yeast Saccharomyces carlsbergensis PTCC 5051, and received as a lyophilized culture YEDPA environment and then the proliferation of malt extract broth is used. The effects of pH, temperature, kinetics and isotherm of lead on metal uptake were evaluated by the SC. Absorption maximum at about pH 5 the optimum temperature was 25 degrees Celsius. A kinetic study showed that batch biosorption of lead to rapid removal by the biomass of Saccharomyces was the first test was done in less than 30 minutes. Method using FT-IR, surface functional groups of fungi were identified. With active and passive absorption of lead by Saccharomyces has revealed more attracted by the yeast is active. With yeast and control yeast on the autoclave, the pre-treated with sodium azide and 2 and 4 DNP nitro phenol showed that claims of capturing, respectively, 0/68, 0/12, 0/44 and 0/48 mmol g was obtained. Results showed that, even using the passive yeast are suitable for the absorption of lead

                                                                     . Keywords: Biosorption, lead, Saccharomyces carlsbergensis, 2, 4-Dinitrophenol, Sodium azide