[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 28, Issue 2 (6-2020) ::
Journal of Ilam University of Medical Sciences 2020, 28(2): 21-31 Back to browse issues page
Effects of Combination Treatment of Cisplatin and Titanium Dioxide Nanoparticles on the BCL2L12 Gene in Prostate Cancer Cells
Azam Emadi1 , Mohammad Javad Mokhtari * 2
1- Dept of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran
2- Dept of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran , mj.mokhtari@gmail.com
Abstract:   (2104 Views)
Introduction: The combined therapy of cancer is more effective than using a single medication for the treatment of cancer. This study aimed at investigating the anticancer effects of cisplatin and cisplatin in combination with Titanium dioxide (TiO2) nanoparticles on the PC-3 prostate cancer cells.
Materials & Methods: The PC-3 cells were cultured in a RPMI1640 medium. Cell viability was assessed by MTT assay during 24, 48, and 72 h, and IC50 was determined. The RNA was extracted, and then the cDNA was synthesized. The expression level of BCL2L12 gene was compared to that of the TBP reference gene using Real-Time Polymerase Chain Reaction method.
Findings: Cisplatin and cisplatin with TiO2 nanoparticles exerted a dose and time dependent inhibitory effect on the viability of PC-3 cells. The expressions of the BCL2L12 gene in cisplatin-treated PC-3 cells at 24, 48, and 72 h were 3.58, 0.08, and 0.17, respectively. Moreover, the corresponding values in cisplatin-treated cells with TiO2 nanoparticles (10μg/ml) were 0.09, 0.05, and 0.02 at 24, 48, and 72 h, respectively, and in cisplatin-treated cells with TiO2 nanoparticles (25μg/ml) were 0.54, 0.04, and 0.07 at 24, 48, and 72 h, respectively (P<0.05).
Discussion & Conclusions: This study revealed that simultaneous treatment with cisplatin and TiO2 nanoparticles (10μg/ml) at low concentration (6.2 and 12.5) can cause more cell death than cisplatin treatment alone. This may be due to the facilitation of cisplatin entry into the cell in the presence of TiO2 nanoparticles.
Keywords: Apoptosis, BCL2L12, Cisplatin, Prostate Cancer, TiO2 Nanoparticles
Full-Text [PDF 872 kb]   (835 Downloads)    
Type of Study: Research | Subject: genetic mlvkly
Received: 2019/10/29 | Accepted: 2019/12/10 | Published: 2020/06/30
1. Azizi F, Hatami H, Janghorbani M. Epidemiology and control of common diseases in Iran 2th ed. Tehran Eshtiagh Publications. 2000;P.602-16.
2. Azizi F, Soleimani M, Hosseini SY, Nik SA, Hosseini J. Gene polymormisms and prostate cancera systematic review. MHJ 2018;2:6.
3. Wang J, Chen B, Chen J, Cai X, Xia G, Liu R, et al. Synthesis and antitumor efficacy of daunorubicin-loaded magnetic nanoparticles. Int J Nanomed2011;6:203-11. doi: 10.2147/IJN.S16165
4. Larsen ME, Rowntree J, Young AM, Pearson S, Smith J, Gibson OJ, et al., editors. Chemotherapy side effect management using mobile phones. 30th Ann Int Con IEEE Eng Med Biol Soc2008; France.
5. Schneider S, Reißner T, Ziv O, Livneh Z, Carell T. Translesion synthesis of 1, 3‐GTG cisplatin DNA lesions. Chembiochem 2010;11:1521-4. doi: 10.1002/cbic.201000211
6. Astolfi L, Ghiselli S, Guaran V, Chicca M, Simoni E, Olivetto E, et al. Correlation of adverse effects of cisplatin administration in patients affected by solid tumours a retrospective evaluation. Oncol Rep2013;29:1285-92. doi: 10.3892/or.2013.2279
7. Rayford C, Schatz G, Shuford K. Optical properties of gold nanospheres. Nanoscape 2005;2:27-33.
8. Xu L, Wu X, Hu C, Zhang Z, Zhang L, Liang S, et al. A meta-analysis of combination therapy versus single-agent therapy in anthracycline and taxane-pretreated metastatic breast cancer: results from nine randomized Phase III trials. Oncol Targ Ther 2016;9:4061-74. doi: 10.2147/OTT.S101423
9. He C, Chan C, Weichselbaum RR, Fleming GF, Yamada SD, Lin W. Nanomedicine for combination therapy of cancer. Ebio Med 2015;2:366-7. doi: 10.1016/j.ebiom.2015.05.013
10. Yang MC, Loh JK, Li YY, Huang WS, Chou CH, Cheng JT, et al. Bcl2L12 with a BH3-like domain in regulating apoptosis and TMZ induced autophagy a prospective combination of ABT-737 and TMZ for treating glioma. Int J Oncol 2015;46:1304-16. doi: 10.3892/ijo.2015.2838.
11. Chou CH, Chou AK, Lin CC, Chen WJ, Wei CC, Yang MC, et al. GSK3β regulates Bcl2L12 and Bcl2L12A anti apoptosis signaling in glioblastoma and is inhibited by LiCl. Cell Cycle2012 ;11:532-42. doi: 10.4161/cc.11.3.19051
12. Stegh AH, Brennan C, Mahoney JA, Forloney KL, Jenq HT, Luciano JP, et al. Glioma oncoprotein Bcl2L12 inhibits the p53 tumor suppressor. Gen Dev 2010;24:2194-204. doi: 10.1101/gad.1924710
13. Hong Y, Yang J, Wu W, Wang W, Kong X, Wang Y, et al. Knockdown of BCL2L12 leads to cisplatin resistance in MDA-MB-231 breast cancer cells. Biochim Biophys Acta 2008;1782:649-57. doi: 10.1016/j.bbadis.2008.09.008
14. Florou D, Papadopoulos IN, Scorilas A. Molecular analysis and prognostic impact of the novel apoptotic gene BCL2L12 in gastric cancer. Biochem Biophys Res Com 2010;391:214-8.doi: 10.1016/j.bbrc.2009.11.034
15. Mehrafsar A, Mokhtari MJ. Effect of exposure to quran recitation on cell viability cell migration and BCL2L12 gene expression of human prostate adenocarcinoma cell line in Culture. Health Spirit Med Eth 2018;5:46-52.doi: 10.29252/jhsme.5.4.46
16. Kim IS, Baek M, Choi SJ. Comparative cytotoxicity of Al2O3 and CeO2 and TiO2 and ZnO nanoparticles to human lung cells. J Nanosci Nanotechnol 2010;10:3453-8. doi: 10.1166/jnn.2010.2340
17. Mokhtari MJ, Koohpeima F, Mohammadi H. A comparison inhibitory effects of cisplatin and MNPs PEG cisplatin on the adhesion capacity of bone metastatic breast cancer. Chem Biol Drug Des 2017;90:618-628. doi: 10.1111/cbdd.12985
18. Mokhtari MJ, Hoseineian Z, Koohpeima F, Akbarzadeh A, Hashemi M, Mahdian R, et al. [Evaluation of cisplatin and cisplatin-loaded magnetic iron oxide nanoparticles on BCL2 and BAX genes in the breast cancer T47D Cell Line]. Pathobiol Res2013;15:75-87. (Persian)
19. Hassanzadeh H, Rezaeitavariani M , Semnani V , Jabbariarfaie A , Seyyedi SS, Jafarzadeh L. Radiation sensitivity study of titanium nanoparticles in irradiation of human gastric cancer cells. J Ilam Uni Med Sci2013;20:121-8.
20. Masumi H, Hasanzadeh H, Rezaeitavirani M, Jadidi M, Nikoofar A, Nasr R, et al. [Effect of nano titana in radiotherapy An in vivo animal study]. J Mazandaran Uni Med Sci 2017;27:1-13. (Persian)
21. Wang Y, Cui H, Zhou J, Li F, Wang J, Chen M, et al. Cytotoxicity DNA damage and apoptosis induced by titanium dioxide nanoparticles in human non small cell lung cancer A549 cells. Environ Sci Pollut Res Int 2015;22:5519-30. doi: 10.1007/s11356-014-3717-7
22. Chellappa M, Anjaneyulu U, Manivasagam G, Vijayalakshmi U. Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method. Int J Nanomed2015;10:31-41.doi: 10.2147/IJN.S79978
23. Wahba J, Natoli M, Whilding LM, Parentepereira AC, Jung Y, Zona S, et al. Chemotherapy induced apoptosis autophagy and cell cycle arrest are key drivers of synergy in chemo-immunotherapy of epithelial ovarian cancer. Cancer Immunol Immunother 2018;67:1753-1765. doi: 10.1007/s00262-018-2199-8
24. Siervosassi R, Marrangoni A, Feng X, Naoumova N, Winans M, Edwards R, et al. Physiological and molecular effects of Apo2L/TRAIL and cisplatin in ovarian carcinoma cell lines. Cancer Lett 2003;190:61-72.doi: 10.1016/s0304-3835(02)00579-7
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Emadi A, Mokhtari M J. Effects of Combination Treatment of Cisplatin and Titanium Dioxide Nanoparticles on the BCL2L12 Gene in Prostate Cancer Cells. Journal of Ilam University of Medical Sciences 2020; 28 (2) :21-31
URL: http://sjimu.medilam.ac.ir/article-1-6118-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 2 (6-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.13 seconds with 31 queries by YEKTAWEB 4538