Effect of Endurance Training on the Expression of the Muscle Fibronectin Type III Domain-Containing Protein
 5 and CTRP15 Levels in the Male Rats

Vosadi, Elham; Barzegar, Hamed

doi:10.29252/sjimu.28.1.78

[Home ] [Archive]

[ فارسی ]

Journal of Ilam University of Medical Sciences

Main Menu

Home

Journal Information

Articles archive

Publication Ethics

Peer Review Process

Indexing Databases

For Authors

For Reviewers

Subscription

Contact us

Site Facilities

Google Scholar Metrics

	All	Since 2020
Citations	6929	3483
h-index	28	19
i10-index	209	82

Search in website

Receive site information

Registered in

Volume 28, Issue 1 (3-2020)

Journal of Ilam University of Medical Sciences 2020, 28(1): 78-88

Back to browse issues page

Effect of Endurance Training on the Expression of the Muscle Fibronectin Type III Domain-Containing Protein 5 and CTRP15 Levels in the Male Rats

Elham Vosadi ^*

¹, Hamed Barzegar²

1- shahrood industrial university , e.vosadi@yahoo.com
2- university of tehran

Abstract: (3160 Views)

Introduction: The effect of exercise on skeletal muscle-derived factors and their effects on the body's metabolism has recently been the focus of attention. This study aimed to investigate the effect of endurance training on the gene expression of the muscle Fibronectin type III domain-containing protein 5 and CTRP15 levels in the male rats.

Materials & Methods: This experimental study included 16 male adult Wistar rats with 8 weeks of age and mean weight of 213±15 gr. The rats were divided into two groups of endurance training (n=8) and control (n=8). The rats in the training group had 4-week endurance training sessions (5 sessions per week) including running on a treadmill for rodents for 45 min at the head of a certain time during the day and at the same time. On the other hand, the control group had no exercise. Initially, the Soleus muscle was homogenized and the expression of Fibronectin type III domain-containing protein 5 and CTRP15genes were measured by real-time polymerase chain reaction analysis. Subsequently, Irisin and insulin levels were measured using enzyme-linked immunosorbent assay and glucose levels were assessed by glucose oxidase. The data were analyzed by independent t-test and a p-value less than 0.05 was considered statistically significant.

Findings: The results of this study showed a significant difference between the training and control groups regarding the expression of Fibronectin type III domain-containing protein 5 and CTRP15 gene in muscle after the endurance exercise (P=0.034 and P=0.048). In addition, the training group had higher Irisin serum level, compared to the control group (P=0.029). Although, insulin resistance obtained a 25% difference after four weeks of endurance training, it was not significant (P=0.500).

Discussion & Conclusions: According to the results of the present study, it seems that mid-term endurance training can improve metabolism by increasing the levels of myokines.

Keywords: CTRP15, Endurance training, Fibronectin type III domain-containing protein 5, Insulin resistance, Rat

Full-Text [PDF 980 kb] (1219 Downloads)

Type of Study: Research | Subject: Physical Education
Received: 2019/10/27 | Accepted: 2020/01/12 | Published: 2020/03/29

References

1. Pedersen L, Hojman P. Muscle to organ cross talk mediated by myokines. Adip J 2012; 1:164-7. doi.10.4161/adip.20344

2. Peterson JM, Mart R, Bond CE. Effect of obesity and exercise on the expression of the novelmyokines myonectin and fibronectin type III domain containing 5. Peer J 2014; 10:1-16. doi.10.7717/peerj.605

3. Bonen A, Chabowski A, Luiken JJFP, Glatz JFC. Mechanisms and regulation of protein-mediated cellular fatty acid uptake molecular biochemical and physiological evidence. APS J 2007; 22: 15-28. doi.002.190.032.243

4. 40Seldin MM, Peterson JM, Byerly MS, Wei Z, Wong GW. Myonectin CTRP15 a novel myokine that links skeletal muscle to systemic lipid homeostasis. Biol Chem J2012; 287: 11968–80. doi.10.1074/jbc.M111.336834

5. Tomtang Y, Hu T, Arterburn M, Boyle B, Bright JM, Palencia S, et al. The complete complement of C1qdomain containing proteins in homo sapiens. Genom J 2005; 86: 100-11. doi.10.1016/j.ygeno.2005.03.001).

6. Xu X, Ying Z, Cai M, Xu Z, Li Y, Jiang SY, et al. Exercise ameliorates high fat diet induced metabolic and vascular dysfunction andincreases adipocyte progenitor cell population inbrown adipose tissue. Am J Physiol Regul Int Comp 2011; 300: 1115-25 doi.10.1152/ajpregu.00806.2010

7. Bonen A, Chabowski A, Luiken JJFP and Glatz JFC. Is membranetransport of FFA mediated by lipid, protein, or both? Mechanisms andregulation of protein-mediated cellular fatty acid uptake molecular,biochemical, and physiological evidence. Physiol J 2007; 22:15-29. doi.10.1152/physiologyonline.2007.22.1.15

8. Kaminski M, Kippen J, Gomulska A, Smyrak J, Karolewski M, Bielawska L, Wysocka E, Cymerys M. Myonectin serum concentration changes after short-term physical activity among young, healthy people. Med Res J 2019; 4 :41-5 (10.5603/MRJ.a2019.0002).

9. Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1alpha dependentmyokine that drives brown fat like developmentof white fat and thermogenesis. Nature J 2012; 481: 463-8. doi.10.1038/nature10777

10. Villarroya F. Irisin, turning up the heat. Cell Metab J 2012; 15: 277-8. doi.10.1016/j.cmet.2012.02.010

11. Lifang Si, Hongxi Liu, Mengyun Li, Guangxin Wang. Study on the relationship between FNDC5 protein expression and aerobic exercise test in Rats. Arch Lat Am Nut 2019; 69: 237-43.

12. Norheim F, Langleite TM, Hjorth M. The effects of acute and chronic exercise on PGC-1𝛼, irisin and browning ofsubcutaneous adipose tissue in Humans. FEBS J 2014; 281: 739-49 doi.10.1111/febs.12619

13. Pang M, Yang J, Rao J, Wang H, Zhang J, Wang S, et al. Time dependent changes in increased levels of plasma irisin and muscle PGC-1α and FNDC5 after exercise in Mice. Tohoku J Exp Med 2018; 244: 93-103. doi.10.1620/tjem.244.93

14. Gamas L, Matafome P, and Seica R. Irisin and myonectin regulation in the insulin resistant muscle: implications to adipose tissue muscle crosstalk. J Diabete Res 2015;2: 1-8. doi.10.1155/2015/359159

15. Nishida T, Tsuji S, Tsujii M, Arimitsu S, Haruna Y, ImanoE. Oral glucose tolerance test predicts prognosisof patients with liver cirrhosis. Am J Gastroenterol 2006; 101: 70-5. doi.10.1111/j.1572-0241.2005.00307.x

16. Pourranjbar M, Arabnejad N, Naderipour KH, Rafie F. [Effects of aerobic exercises on serum levels of myonectin and insulin resistance in obese and overweight Women]. J Med Life2018; 11: 381-6. (Persian) doi.10.25122/jml-2018-0033

17. Bashar R. Method of blood sampling and anesthesia for rodent animals in physical education research. 2th ed. Ins Phys Edu Sport Scie Publication. 2006;P.123-9.

18. Jenkins NT, Padilla J, Esquivel AA, Bayless DS, Martin JS, Leidy HJ, Booth FW, Rector RS, Laughlin MH. Effects of endurance exercise training, metformin, and their combination on adipose tissue leptin and IL10 secretion in OLETF Rats. J Appl Physiol 2012; 113: 1873-83. doi.10.1152/japplphysiol.00936.2012

19. Bluher S, Panagiotou G, Petroff D. Effects of a 1year exercise and lifestyle intervention on irisin, adipokines, and inflammatory markers in obese Children. Obesity J 2014; 22: 1701-8 doi.10.1002/oby.20739

20. Brenmoehl J, Albrecht E, Komolka K. Irisin is elevated in skeletal muscleand serum of mice immediately after acute exercise. Int J Biol Sci J2014;10:338-49 doi.10.7150/ijbs.7972

21. Timmons JA, Baar K, Davidsen PK, Atherton PJ. Is irisin a human exercise gene? Nature J 2012; 488: 9-10 doi.10.1038/nature11364

22. Tiraby C, Tavernier G, Lefort C, Larrouy D, Bouillaud F, Ricquier D. Acquirement of brown fat cell features by human white adipocytes. J Biol Chem 2003; 278: 33370-6. doi.10.1074/jbc.M305235200

23. Seldin MM, Peterson JM, Byerly MS, Wei Z, Wong GW. Myonectin CTRP15 a novel myokine that linksskeletal muscle to systemic lipid homeostasis. Biol Chem J 2012; 287: 11968-80. doi.10.1074/jbc.M111.336834

24. Lim S, Choi SH, Koo BK, Kang SM, Yoon JW,Jang HC, et al. Effects of aerobic exercise training on c1q tumor necrosis factor related protein isoform 5 myonectin association with insulin resistance andmitochondrial DNA density in Women. Clin Endocrinol Metab J2012; 97: 88-93. doi.10.1210/jc.2011-1743

25. Park SY, Choi JH, Ryu HS, Pak YK, Park KS, Lee HK, et al. C1q tumor necrosis factor α-related protein isoform 5 is increased in mitochondrial DNA-depleted myocytes and activates AMP-activated protein kinase. J Biol Chem 2009; 284:27780-9. doi.10.1074/jbc.M109.005611

26. Gamal MM, Tork OM, Eshra MA, Magdy S, Rashed LA. Role of endogenous irisin a novel myokine in cognitive functions and insulin sensitivity in exercised diabetic Rats. MEDC J2016;22: 136-146. doi.10.4103/1687-4625.195886

27. Huth C, Marette A, Tremblay A. Plasma irisin is associated with insulinresistance in Men. FASEB J 2014; 28: 854.

28. Liu J, Wong M, Ching TW. Lower circulating irisin is associated withtype 2 diabetes mellitus. Journal of Diabetes and its Complications. 2013; 27:365-9 doi.10.1016/j.jdiacomp.2013.03.002

29. Han TS, Bijan FC. Separate associations of waist and hip circumference with life style factors. Int J Epidemiol 1999; 27:422-30. doi.10.1093/ije/27.3.422

30. Hejiden GV, Wang ZJ, Chu ZH. A 12-Week aerobic exercise program reduces hepatic fat accumulation and insulin resistance in obese hispanic adolescents. Epidemiol J 2010; 18: 384-91 doi.10.1038/oby.2009.274

Send email to the article author

Add your comments about this article

‎ 10.29252/sjimu.28.1.78

Mendeley

Zotero

RefWorks

Vosadi E, Barzegar H. Effect of Endurance Training on the Expression of the Muscle Fibronectin Type III Domain-Containing Protein 5 and CTRP15 Levels in the Male Rats. J. Ilam Uni. Med. Sci. 2020; 28 (1) :78-88
URL: http://sjimu.medilam.ac.ir/article-1-6111-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 28, Issue 1 (3-2020)

Back to browse issues page

Persian site map - English site map - Created in 0.14 seconds with 41 queries by YEKTAWEB 4671