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:: Volume 28, Issue 4 (10-2020) ::
Journal of Ilam University of Medical Sciences 2020, 28(4): 25-35 Back to browse issues page
The Study of Expression of Casp3/Bax Genes in HT29 Colon Cancer Cell Line with Lactobacillus rhamnosus Co-Culturing
Anahita ZibasazTalebi1 , Changiz Ahmadizadh * 2
1- Dept of Microbiology, Ahar Branch, Islamic Azad University, Ahar, Iran
2- Dept of Microbiology, Ahar Branch, Islamic Azad University, Ahar, Iran , dr_ahmadizadeh@yahoo.com
Abstract:   (1851 Views)
Introduction: Colon cancer has spread to developed countries. Probiotic bacteria can play an important role in reducing cancer and treating colon cancer. The aim of this study was to determine the effect of adjacent bovine Lactobacillus rhamnose culture on the expression of casp3 and bax genes in cancer cell.
 
Materials & Methods: In this experimental study after bacterial culture, supernatant and bacterial extract were prepared and the cells were treated with these materials. The effects of cell cytotoxicity of the cell line on the HT29 cell line were investigated using MTT method. After extraction of RNA and preparation of cDNA, the expression of bax, casp3 genes in the HT29 cell line was investigated using Real Time PCR.
 
Findings: The results of MTT assay showed that Lactobacillus ramnose at a concentration of 10 μg/ml reduced the survival of HT-29 cells by 51.49±8.32%, and the coloring results detection showed that treatment of HT29 cells with Lactobacillus rhamnosis caused qualitative changes in cell apoptosis. Real-time PCR results showed that lactobacillus rhamnose bacteria significantly increased the expression of bax genes (4.2±0.22) and casp3 (6.88±0.92) compared to control gene in cancer cells of the HT29 colon (P<0.05).
 
 Discussion & Conclusions: The results of this study showed that lactobacillus ramose bacteria increase the expression of bax, cas3 gene and cause induction of apoptosis in HT-29 cell line. Therefore, further studies can be used as an anti-cancer probiotic product in the treatment and prevention of colon cancer.
Keywords: Colon cancer, Gene expression, Lactobacillus rhamnose
Full-Text [PDF 787 kb]   (1232 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2019/09/25 | Accepted: 2020/04/28 | Published: 2020/10/31
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ZibasazTalebi A, Ahmadizadh C. The Study of Expression of Casp3/Bax Genes in HT29 Colon Cancer Cell Line with Lactobacillus rhamnosus Co-Culturing. Journal of Ilam University of Medical Sciences 2020; 28 (4) :25-35
URL: http://sjimu.medilam.ac.ir/article-1-6020-en.html


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Volume 28, Issue 4 (10-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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