[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 28, Issue 2 (6-2020) ::
Journal of Ilam University of Medical Sciences 2020, 28(2): 38-49 Back to browse issues page
The Ameliorative Effects of Achillea millefolium Inflorescence Hydro Alcoholic Extract on Sperm Parameters in Male Rats Exposed to Diethyl-Hexyl–Phthalate
Zahra Karimpour1 , Shapour Hasanzadeh * 2, Mazdak Razi3
1- Dept of Comparative Histology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2- Dept of Veterinary Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , shasanzadehs@yahoo.com
3- Dept of Veterinary Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abstract:   (1696 Views)
Introduction: Esters of phthalates, particularly di-(2-ethylhexyl)-phthalate (DEHP) generally utilized in plastic industry are environmental hazardous pollutants, has toxic effects on body systems, including reproductive system. The aim of this study was to evaluate the effects of Achillea millefolium on rats’ sperm exposed to DEHP.
 
Materials & Methods: In this study, 45 male rats were randomly divided into 9 groups. 1- Control, 2- sham, received corn oil, 3- received 75mg/kg/day hydro alcoholic extract of Achillea millefolium inflorescence, 4- Low-dose diethyl-hexyl–phthalate (DEHP1), 5- Low-dose diethyl-hexyl–phthalate+Achillea millefolium, (DEHP1+A) 6- Medium-dose diethyl-hexyl–phthalate (DEHP2), 7- Medium-dose diethyl-hexyl–phthalate + Achillea millefolium, (DEHP2+A) 8- High-dose diethyl-hexyl–phthalate (DEHP3), 9- High-dose diethyl-hexyl–phthalate + Achillea millefolium (DEHP3+A). All of groups except the control group were treated by gavage for 40 days. After euthanized, the epididymal sperms are collected to investigate the sperm parameters. Data were analyzed using ANOVA and Tukey post hoc tests.
 
Findings: di-(2-ethylhexyl)-phthalate significantly (P<0.05) decreased the sperm parameters including, count, viability, motility and maturation of the nucleus and significantly increased the breakage of sperm nuclear DNA in comparison to control group. Achillea millefolium inflorescence hydro alcoholic extract had a positive effect on these parameters.
 
Discussion & Conclusions: More likely Achillea millefolium inflorescence hydro alcoholic extract has antioxidant effects thus reduces the injurious effects of di-ethylhexyl phthalate on sperm parameters.
 
Keywords: di-(2-ethylhexyl)-phthalate, Achillea millefolium inflorescence, Rat, Sperm
Full-Text [PDF 869 kb]   (593 Downloads)    
Type of Study: Research | Subject: toxicology
Received: 2019/09/23 | Accepted: 2020/01/13 | Published: 2020/06/30
References
1. Bhasin S, Kretser D, Baker H. Clinical review 64 pathophysiology and natural history of Male infertility. J Clin Endocrinol Metab 1994; 79:1525.
2. Bayasgalan G, Naranbat D, Radnaabazar J, Lhagvasuren T, Rowe P. Male infertility risk factors in Mongolian Men. Asian J Androl 2004; 6:305-11.
3. Carbone S, Szwarcfarb B, Ponzo O, Reynoso R, Cardoso N, Deguiz L, et al. Impact of gestational and lactational phthalate exposure on hypothalamic content of amino acid neurotransmitters and FSH secretion in peripubertal male Rats. Neurotoxicology 2010; 31:747-51. doi.org/10.1016/j.neuro.2010.06.006
4. Quinnies KM, Harris EP, Snyder RW, Sumner SS, Rissman EF. Direct and transgenerational effects of low doses of perinatal di-2-ethylhexyl phthalate on social behaviors in mice. PLos One 2017; 12:41-8.
5. Carbone S, Szwarcfarb B, Ponzo O, Reynoso R, Cardoso N, Deguiz L, et al. Impact of gestational and lactational phthalate exposure on hypothalamic content of amino acid neurotransmitters and FSH secretion in peripubertal male Rats. NeuroToxicology 2010;31: 747-51.
6. Liu T, Li N, Zhu J, Yu G, Guo K, Zhou L, et al. Effects of di-2-ethylhexyl phthalate on the hypothalamus-pituitary-ovarian axis in adult female rats. Reproductive toxicology 2014; 46:141-7. doi.org/10.1016/j. reprotox.2014.03.006
7. Potrich FB, Allemand A, Silva LM, Santos AC, Baggio CH, Freitas CS, et al. Antiulcerogenic activity of hydroalcoholic extract of Achillea millefolium L. involvement of the antioxidant system. J Ethnopharmacol 2010; 130:85-92.
8. Saeidnia S, Gohari A, Mokhber N, Kiuchi F. A review on phytochemistry and medicinal properties of the genus Achillea. Daru 2011; 19:173-86.
9. Cavalcanti AM, Baggio CH, Freitas CS, Rieck L, Sousa RS, Silva JE, et al. Safety and antiulcer efficacy studies of Achillea millefolium L. after chronic treatment in wistar Rats. J Ethnopharmacol2006; 107:277-84. doi.org/10.1016/j. jep.2006.03.011
10. Karabulut G, Barlas N. Genotoxic histologic immunohistochemical morphometric and hormonal effects of di-2-ethylhexyl-phthalate on reproductive systems in pre pubertal male Rats. Toxicol Res 2018; 7:859-73. doi. 10.1039/C8TX00045J
11. Rashidi I, Taherimoghadam M, Mozaffari A. [Study of anti-inflammatory and healing effects of Achillea millefolium in the treatment of indomethacin induced gasthic ulcer in Rat]. Qazvin Uni Med Sci J2005; 8:9-13. (Persian)
12. Agarwal DK, Eustis S, Reel JR, Kluwe WM. Effects of di 2-ethylhexyl phthalate on the gonadal pathophysiology, sperm morphology, and reproductive performance of male Rats. Environ Health Perspect 1986; 65:343-50. doi.org/10.1289/ehp.8665343
13. Li X, Fang EF, Scheibye M, Cui H, Qiu L, Li J, et al. di-2-ethylhexyl phthalate inhibits DNA replication leading to hyperparylation SIRT1 attenuation and mitochondrial dysfunction in the testis. Sci Rep2014; 4:6434.Doi.org/10.1038/srep06434
14. Huang XF, Li Y, Gu YH, Liu M, Xu Y, Yuan Y, et al. The effects of di-2-ethylhexyl phthalate exposure on fertilization and embryonic development in vitro and testicular genomic mutation invivo. Plos One2012; 7:22-8.
15. Jurewicz J, Radwan M, Sobala W, Ligocka D, Radwan P, Bochenek M, et al. Human urinary phthalate metabolites level and main semen parameters sperm chromatin structure sperm aneuploidy and reproductive hormones. Rep Toxicol 2013; 42:232-41. doi.org/10.1016/j.reprotox.2013.10.001
16. Pant N, Shukla M, Patel DK, Shukla Y, Mathur N, Gupta YK, et al. Correlation of phthalate exposures with semen quality. Toxicol Appl pharmacol 2008; 231:112-6.doi.org/10.1016/j.taap.2008.04.001
17. Hauser R, Meeker J, Singh N, Silva M, Ryan L, Duty S, et al. DNA damage in human sperm is related to urinary levels of phthalate monoester and oxidative metabolites. Hum Rep2007; 22:688-95. doi.org/10.1093/humrep/del428
18. Zare Z, Eimani H, Mohammadi M, Mofid M, Dashtnavard H. [Histopathological study of di-2-ethylhexyl phthalate on testes in Mouse]. J Mazandaran Uni Med Sci 2009; 19:52-9. (Persian)
19. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fert Ster2003; 79:829-43. doi.org/10.1016/S0015-0282(02)04948-8
20. Gilguzman E, Ollero M, Lopez M, Sharma R, Alvarez J, Thomas JRA, et al. Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum Rep 2001; 16:1922-30. doi.org/10.1093/humrep/16.9.1922
21. Acharya UR, Mishra M, Patro J, Panda MK. Effect of vitamins C and E on spermatogenesis in mice exposed to cadmium. RepToxicol 2008; 25:84-8.doi.org/10.1016/j.reprotox.2007.10.004
22. Zolghadri Y, Fazeli M, Kooshki M, Shomali T, Karimaghaie N, Dehghani M. Achillea Millefolium L. hydro- alcoholic extract protects pancreatic cells by down regulating IL- 1β and iNOS gene expression in diabetic Rats. Int J Mole Cell Med2014; 3:255-62.
23. Alikhanzade M, Tehranipour M. [Investigating the neuroprotective effect of alcholic extracts of Achillea biebersteinii leave on α motoneurons after sciatic nerve compression in Rats]. SSUJ 2014; 22:1323-32. (Persian)
24. Pereira JM, Peixoto V, Teixeira A, Sousa D, Barros L, Ferreira IC, et al. Achillea millefolium L. hydroethanolic extract inhibits growth of human tumor cell lines by interfering with cell cycle and inducing apoptosis. Food Chem Toxicol 2018;118:635-44.doi.org/10.1016/j.fct.2018.06.006
25. Asliranifam N, Hasanzadeh SH, Najafi GH. [The effects of hydroalcholic extract of Achillea Millefolium on invitro fertilization and emberyonic development process in Mice]. Qom Uni Med Sci J 2017; 10: 23-3. (Persian)
26. Najafi RA, Taheri A, Najafi AA, Rouhollahi M. Effect of Achillea millefolium loaded nanophytosome in the post thawing sperm quality and oxidative status of rooster semen. Cryobiology 2018;2: 4-11.
27. Salahipour MH, Hasanzadeh S, Malekinejad H. Ameliorative effects of Achillea millefolium inflorescences alcoholic extract against nicotine-induced reproductive failure in Rat. Exp Toxicol Pathol2017; 69:504-16. doi.org/10.1016/j.etp.2017.04.012
28. Jalali AS, Hasanzadeh S, Malekinejad H. Achillea millefolium inflorescence aqueous extract ameliorates cyclophosphamide-induced toxicity in rat testis: stereological evidences. Chinese J Natural Med2012, 10: 247-54. doi. 10.1016/S1875-5364(12)60050-8
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karimpour Z, Hasanzadeh S, Razi M. The Ameliorative Effects of Achillea millefolium Inflorescence Hydro Alcoholic Extract on Sperm Parameters in Male Rats Exposed to Diethyl-Hexyl–Phthalate. Journal of Ilam University of Medical Sciences 2020; 28 (2) :38-49
URL: http://sjimu.medilam.ac.ir/article-1-6017-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 2 (6-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.14 seconds with 31 queries by YEKTAWEB 4541