[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 28, Issue 3 (8-2020) ::
Journal of Ilam University of Medical Sciences 2020, 28(3): 53-62 Back to browse issues page
Anticancer Effect of Heracleum persicum Alcoholic Extract and Leishmania Major Promastigute in Comparison with Doxorubicin in MCF7 Breast Cancer Cell Line and Natural HU02 Fibroblast
Zahra Bagheri-Hosseinabadi1 , Fatemeh Javani Jouni2 , Seyedeh Nahid Sajjadi3, Hossein Vazini * 4, Ameneh Elikaei5
1- Dept of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
2- Dept of Biomedical Engineering, Faculty of Health, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
3- Dept of Biology, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran
4- Dept of Nursing, Faculty of Basic Sciences, Hamadan Branch, Islamic Azad University, Hamadan, Iran , hossein_vazini@yahoo.com
5- Dept of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Abstract:   (2662 Views)
Introduction: Breast cancer is the most common cancer among women. Doxorubicin is one of the most important chemotherapy drugs in the treatment of tumors with many side effects. Today, herbal compounds make up about one-third of the total available drugs. Heracleum persicum has anti-cancer effects. Moreover, in recent years, there has been an interest in the study of new drug targets, such as the use of parasitic compounds in cancer treatment. Therefore, this study aimed to investigate the anticancer effects of Heracleum persicum alcoholic extract and Leishmania major promastigute in comparison with doxorubicin in MCF7 breast cancer cell line and HU02 fibroblast.
Materials & Methods: Breast and fibroblast cell lines were exposed to logarithmic concentrations of doxorubicin, Heracleum persicum extract, and Leishmania promastigute separately and in combination. The MTT was used to determine the inhibitory concentration of this herb extract, medicine, and the parasite. The data were analyzed in SPSS software. Ethics code: IR.SKUMS.0063-99
Findings: The results showed that an increase in the concentration of doxorubicin, Heracleum persicum extract, and Leishmania promastigute individually and in combination led to a decrease in the percentage of live fibroblast cells and MCF7. The highest percentage of the decreased live cells is observed in the combined application of Heracleum persicum extract, Leishmania promastigute, and doxorubicin.
Discussion & Conclusions: Heracleum persicum extract and Leishmania promastigute can have significant inhibitory effects on the percentage of live fibroblast cells and MCF7, which is more pronounced in living cells of MCF7.
Keywords: Breast cancer, Doxorubicin, Heracleum persicum alcoholic extract, Leishmania major promastigute
Full-Text [PDF 677 kb]   (1100 Downloads)    
Type of Study: Research | Subject: medical parasitology
Received: 2019/05/7 | Accepted: 2020/01/7 | Published: 2020/08/31
1. Hanbyoel L, Wonshik H. Unique features of young age breast cancer and its management. J Breast Cancer2014; 17: 301-30. doi.10.4048/jbc.2014.17.4.301
2. Comşa S, Cîmpean A M, Raica M. The story of MCF-7 breast cancer cell line 40 years of experience in research. Anticancer Res2015; 35: 3147-54. doi.0250-7005/2015 $2.00+.40
3. Wakharde AA, Awad AH, Bhagat A, Karuppayil SM. Synergistic activation of doxorubicin against cancer a review. Am J Clin Microbiol Antimicrob2018; 1: 1009.
4. Mizutani H, Oikawa S, Hiraku Y, Murata M, Kojima M, Kawanishi S. Distinct mechanisms of site specific oxidative DNA damage by doxorubicin in the presence of copper II and NADPH cytochrome P450 reductase.Cancer Sci 2003;94:686-91. doi.10.1111/j.1349-7006. 2003.tb01503. x.
5. Ottewell PD, Woodward JK, Lefley DV, Evans CA, Coleman RE, Holen I. Anticancer mechanisms of doxorubicin and zoledronic acid in breast cancer tumor growth in bone. Mole Cancer Ther 2009; 8: 2821-32. doi.10.1158/1535-7163.MCT-09-0462
6. Kelleni MT, Amin EF, Abdelrahman AM. Effect of metformin and sitagliptin on doxorubicin-induced cardiotoxicity in Rats impact of oxidative stress inflammation and apoptosis. J Toxicol 2015; 1-8. doi. 10.1155/2015/424813
7. Rivankar S. An overview of doxorubicin formulations in cancer therapy. J Cancer Res Therap 2015; 10: 853. doi.10.4103/0973-1482.139267
8. Kalantari N, Ahangardarabi Z, Siadati S, Nikbakhsh N, Ghasemi M, Ghaffari T, et al. Detection of Toxoplasma gondii DNA in malignant breast tissues in breast cancer patients. Int J Mole Cell Med2017; 6: 190-6. doi.10.22088/acadpub.BUMS.6.3.190
9. Callejas B.E, Martínez-Saucedo D, Terrazas L.I. Parasites as negative regulators of cancer. Biosci Rep 2018; 38: 20180935. doi. 10.1042/BSR20180935
10. Soltanian S, Sheikhbahaei M, Mohamadi N. Cytotoxicity evaluation of methanol extracts of some medicinal plants on p19 embryonal carcinoma cells. J Appl Pharm Sci 2017; 7: 142-9. doi. 10.7324/JAPS.2017.70722
11. Shariatifar N, Mostaghim T, Afshar A, Mohammadpourfard I, Sayadi M, Rezaei M. Antibacterial properties of essential oil of Heracleum persicum Golpar and foodborne pathogens. Int J Enter Path2017; 5: 41-4. doi.10.15171/ijep.2017.10
12. Taghizabet N, Mangoli E, Anbari F, Masoodi SA, Talebi AR, Mazrooei M. The effect of Heracleum persicum Golpar oil and alcoholic extracts on sperm parameters and chromatin quality in mice. Int J Rep Biomed Yazd, Iran 2016; 14: 365-70.
13. Silveira FT, Blackwell JM, Ishikawa EA, Braga R, Shaw JJ, Quinnell RJ, et al. T cell responses to crude and defined leishmanial antigens in patients from the lower Amazon region of Brazil infected with different species of Leishmania of the subgenera Leishmania and Viannia. Parasite Immunol1998; 20: 19-26. doi.10.1046/j.1365-3024.1998.t01-1-00126.x
14. Bodo J, Chovancova J, Hunakova L, Sedlak J. Enhanced sensitivity of human ovarian carcinoma cell lines A2780 and A2780/CP to the combination of cisplatin and synthetic isothiocyanate ethyl 4-isothiocyanatobutanoate. Neoplasma 2005; 52: 510-6.
15. Azizi M, Bahadori M, Azizi F. History of cancer in Iran. Arch Iran Med 2013; 16: 613-22.
16. Thorn CF, Oshiro C, Marsh S, Hernandez T, Mcleod H, Klein TE, Altman RB. Doxorubicin pathways: pharmacodynamics and adverse effects. Pharm Genom 2011; 21: 440-6. doi. 10.1097/FPC.0b013e32833ffb56
17. Smith L, Watson MB, Kane SLO, Drew PJ, Lind MJ, Cawkwell L. The analysis of doxorubicin resistance in human breast cancer cells using antibody microarrays. Mole Cancer Therap 2006; 2115-21. doi.10.1158/1535-7163.MCT-06-0190
18. Lovitt CJ, Shelper TB, Avery VM. Doxorubicin resistance in breast cancer cells is mediated by extracellular matrix proteins. BMC Cancer2018; 18: 41. doi. 10.1186/s12885-017-3953-6
19. Prakash O, Kumar A, Kumar P. Anticancer potential of plants and natural products a review. Am J Pharmacol Sci2013; 1: 104–115. doi: 10.12691/ajps-1-6-1
20. Bayala B, Bassole IHN, Scifo R, Gnoula C, Morel L. Anticancer activity of essential oils and their chemical components a review. Am J Cancer Res2014; 4: 591-607. doi. 2156-6976/ajcr0001130
21. Sadeghialiabadi H, Ahmad S, Saeidi M, Jafarian A. Cytotoxic effects of the extracts of iranian taxus baccata and cupressus horizentalis on cancer cells. Iranian J Pharmaceut Res2003; 2:107-10. doi. 10.22037/IJPR.2010.21
22. Filiz B, Songul K, Bostanlık D, Gul F, Ceyda Sibel K. Anticancer effect of ferulago mughlea peşmen apiaceae on cancer cell proliferation. Iran J Pharm Res 2016; 15: 501-4.
23. Holohan C, Schaeybroeck S, Longley DB, Johnston PG. Cancer drug resistance an evolving paradigm. Nature Rev Cancer 2013; 13: 714-26. doi.10.1038/nrc3599
24. Mohammadi A, Baradaran B. [Apoptotic effect of the urtica dioica plant extracts on breast cancer cell line MDA- MB- 468]. J Ardabil Uni Med Scie2015; 15: 283-90. (Persian)
25. Elikaei A, Vazini H, Javani F. [Anticancer effects of parasite extracts of leishmaniasis and toxoplasma On resistant cell line A2780-CP and sensitive A2780 to cisplatin]. J Alzahra Uni Appl Biol2018; 31:23-8. doi. 10.22051/JAB.2017.16470.1162 (Persian)
26. Taqavi M, Nemati F, Khan Babaei R. Effect of glacial plant on cancer cell apoptosis. 3th National Con Food Secu Tehran Iran. 2013.
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bagheri-Hosseinabadi Z, Javani Jouni F, Sajjadi S N, Vazini H, Elikaei A. Anticancer Effect of Heracleum persicum Alcoholic Extract and Leishmania Major Promastigute in Comparison with Doxorubicin in MCF7 Breast Cancer Cell Line and Natural HU02 Fibroblast. Journal of Ilam University of Medical Sciences 2020; 28 (3) :53-62
URL: http://sjimu.medilam.ac.ir/article-1-5655-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 3 (8-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.14 seconds with 31 queries by YEKTAWEB 4541