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:: Volume 19, Issue 3 (10-2011) ::
Journal of Ilam University of Medical Sciences 2011, 19(3): 13-20 Back to browse issues page
Frequency of Ompk35 And Ompk36 in Strains of
P Shakib, N Sadeghifard *1, M Zolfaghary, S Gafouryan, A Maleki, R Mohebi, R Ranjbar
1- , sadeghifard@gmail.com
Abstract:   (16209 Views)
: Klebsiella pneumonia is a major nosocomial pathogen causing pneumonia, urinary tract infections, and bacteremia, particularly in immune-compromised patients. The aim of our study was expression of OMPK36 and OMPK35 in ESBLs production and non-ESBLs K.pneumoniae. Materials & Methods: Clinical isolates of K.pneumoniae were identified. ESBLs were assayed by Phenotypic (screening and confirming) and genotypic (PCR) methods. OMPK35 and OMPK36 were tested by polymerase chain reaction and SDS- PAGE. Findings: Our findings showed that 42.3% of K.pneumoniae were ESBLs positive. Twenty-two K.pneumoniae producing ESBLS and Twenty-two non-ESBLs K.pneumoniae were selected for porines detection. We found out that 54.54% and 72.72% of K.pneumoniae producing ESBLS were positive for Ompk35 and Ompk36 respectively, while our findings in non-ESBLs K.pneumoniae showed that 95.94% and 100% of isolates were positive for Ompk35 and Ompk36, respectively. Discussion & Conclusion: Many of clinical isolates of non- ESBL K. pneumoniae had expressed both Ompk35 and Ompk36 porins, while our results in ESBLs positive K.pneumoniae had only showed expression of Ompk36. A few of non-ESBLs and ESBLs K.pneumoniae were found as negative for both OMPK35 and OMPK36. Our findings released that lack of porins in K.pneumoniae are defined as an antibiotic- resistant mechanism in many isolates.
Keywords: ESBLs(Extended spectrum beta-lactamases), Ompk35, Ompk36
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Type of Study: Research | Subject: General
Received: 2012/01/11 | Published: 2011/10/15
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Shakib P, Sadeghifard N, Zolfaghary M, Gafouryan S, Maleki A, Mohebi R et al . Frequency of Ompk35 And Ompk36 in Strains of. Journal of Ilam University of Medical Sciences 2011; 19 (3) :13-20
URL: http://sjimu.medilam.ac.ir/article-1-509-en.html

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Volume 19, Issue 3 (10-2011) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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