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:: Volume 26, Issue 4 (11-2018) ::
Journal of Ilam University of Medical Sciences 2018, 26(4): 175-185 Back to browse issues page
The Inhibitory Effects of Silver Nanoparticles on Bap Gene Expression in Antibiotic-Resistant Acientobacter bumanni Isolates using Real-Time PCR
Tohid Piri Gharaghie1 , Seyed Ataollah Sadat Shandiz * 2
1- Dept of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
2- Dept of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran , atashandiz@yahoo.com
Abstract:   (3452 Views)
Introduction: Acinetobacter bomanni is one of the most common opportunistic pathogens in hospitals that are resistant to many antibiotics due to biofilm production. Nowadays, silver nanoparticles(AgNPs) have received wide attention in medicine due to their physical and chemical properties. This study aimed to evaluate the anti-biofilm activity of silver nanoparticles on antibiotic-resistant A. bumanni strains.
 
Materials & Methods: In this experimental study, A. bumanni was isolates from 100 clinical samples. After identification of A. bummani strains and determination of antibiotic resistant profiles, biofilm-producing isolates were determined using Congo red agar at 37°C after 24 hours. The minimum inhibitory concentration (MIC) of the strains against AgNPs was determined. After 24 hours of exposure of the strains to sub-MIC concentration of AgNPs, RNA extraction and cDNA synthesis were performed. Finally, Bap gene expression was measured using real time PCR method.
Finding: Out of 100 clinical isolates, 12 belonged to A. bummani, and all the strains were resistant to antibiotics, except for colistin. Real-time PCR results show that all the strains had a significant down-regulation in Bap gene expression compared to the control gene (P<0.05). 


Discussion & Conclusions: According to the anti-biofilm effects of AgNPs, it seems that AgNPs can be used as drug candidates in pharmaceutical industries. 


Keywords: Acientobacter bummani, Biofilm, Bap, Silver nanoparticle
Full-Text [PDF 878 kb]   (2268 Downloads)    
Type of Study: Research | Subject: biology
Received: 2018/05/23 | Accepted: 2018/09/12 | Published: 2018/11/15
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Piri Gharaghie T, Sadat Shandiz S A. The Inhibitory Effects of Silver Nanoparticles on Bap Gene Expression in Antibiotic-Resistant Acientobacter bumanni Isolates using Real-Time PCR . Journal of Ilam University of Medical Sciences 2018; 26 (4) :175-185
URL: http://sjimu.medilam.ac.ir/article-1-4977-en.html


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Volume 26, Issue 4 (11-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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