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17-β estradiol Attenuated Hippocampus Oxidative Stress in an Ethidium Bromide-Induced Multiple Sclerosis Model among Adult Male Rats
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Ali reza Ali Hemmati1    , Homeira Hatami * 2, Nazli Khajehnasiri3 |
1- Dept of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2- Dept of Animal sciences, Faculty of biological sciences, Tabriz University, Tabriz, Iran , Homeirahatami@yahoo.com
3- Dept of Animal sciences, Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran |
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Abstract: (4263 Views) |
Introduction: Multiple sclerosis (MS) is a disease of the central nervous system in which myelin is destroyed. Oxidative stress and subsequent apoptosis are recognized as factors involved in the pathogenesis of MS. On the other hand, 17-β-estradiol is well known for its anti-oxidant properties. Therefore, this study aimed to investigate the effect of 17-beta-estradiol on oxidative stress parameters in the experimental model of MS.
Materials & Methods: This study was conducted on 49 Wistar male rats cannulating into the CA1 area of hippocampus. The rats were randomly divided into following groups (n=7): control group, sham group, MS group, estradiol groups, MS+estradiol groups. MS model was induced by intrahippocampal injection of ethidium bromide and estradiol was injected as a pretreatment for 5 days. At the end of experiments, the levels of oxidative stress parameters, such as MDA, Glutathione, and antioxidant enzymes (Glutathione peroxidase, Superoxide dismutase and Catalase)were measured in this study.
Findings: The microinjection of ethidium bromide increased the oxidative capacity and reduced the antioxidant enzyme (SOD GPx and CAT) activity (P<0.05). The pre-treatment of 17-beta-estradiol prevented an increase in oxidative capacity and decreased the activity of antioxidant enzymes in the experimental groups (P<0.05).
Discussion & Conclu sions: The 17-beta-estradiol, as a potent antioxidant, is likely to prevent the increase of oxidative stress indices in the experimental model of MS by removing reactive oxygen species and clearing the tissues from free radicals. |
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| Keywords: Multiple sclerosis, Ethidium bromide, Microinjection, Oxidative stress, 17-beta-estradiol, Hippocampus, Rat |
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Full-Text [PDF 960 kb]
(1281 Downloads)
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Type of Study: Research |
Received: 2017/12/1 | Accepted: 2018/04/4 | Published: 2019/03/15
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